Ligustrum virus A
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1C9IAX3|A0A1C9IAX3_9VIRU 7 kDa protein OS=Ligustrum virus A OX=1899566 PE=3 SV=1
MM1 pKa = 8.16 IEE3 pKa = 3.83 VCLVIACVLLIVASVYY19 pKa = 10.76 LNSVNEE25 pKa = 4.34 TCSITITGEE34 pKa = 3.72 SVRR37 pKa = 11.84 IIGCVQSPEE46 pKa = 3.94 FLDD49 pKa = 5.61 LIGKK53 pKa = 7.7 LKK55 pKa = 10.34 PAGSCC60 pKa = 3.6
Molecular weight: 6.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.674
IPC2_protein 4.902
IPC_protein 4.507
Toseland 4.368
ProMoST 4.596
Dawson 4.431
Bjellqvist 4.584
Wikipedia 4.279
Rodwell 4.355
Grimsley 4.291
Solomon 4.406
Lehninger 4.355
Nozaki 4.558
DTASelect 4.584
Thurlkill 4.393
EMBOSS 4.304
Sillero 4.609
Patrickios 0.299
IPC_peptide 4.418
IPC2_peptide 4.596
IPC2.peptide.svr19 4.586
Protein with the highest isoelectric point:
>tr|A0A1C9IAX3|A0A1C9IAX3_9VIRU 7 kDa protein OS=Ligustrum virus A OX=1899566 PE=3 SV=1
MM1 pKa = 7.54 NSHH4 pKa = 6.66 KK5 pKa = 10.46 KK6 pKa = 10.66 DD7 pKa = 3.25 IVFLLLSVFKK17 pKa = 10.79 KK18 pKa = 10.55 RR19 pKa = 11.84 GGSFPLAICVLIYY32 pKa = 10.43 QLAFNKK38 pKa = 9.78 QVGGGTSTYY47 pKa = 10.22 ARR49 pKa = 11.84 RR50 pKa = 11.84 RR51 pKa = 11.84 RR52 pKa = 11.84 AVSIGRR58 pKa = 11.84 CHH60 pKa = 6.1 RR61 pKa = 11.84 CYY63 pKa = 10.0 RR64 pKa = 11.84 VYY66 pKa = 10.42 PPPYY70 pKa = 10.22 FGTKK74 pKa = 9.72 CDD76 pKa = 4.2 NKK78 pKa = 9.53 TCAPGLSYY86 pKa = 10.74 NVKK89 pKa = 9.81 VEE91 pKa = 3.76 NFIRR95 pKa = 11.84 WGVTEE100 pKa = 5.1 VIPQRR105 pKa = 11.84 AKK107 pKa = 10.76 NKK109 pKa = 7.51 TSEE112 pKa = 4.15 VFRR115 pKa = 11.84 IKK117 pKa = 10.72 PNN119 pKa = 3.29
Molecular weight: 13.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.315
IPC2_protein 9.736
IPC_protein 10.072
Toseland 10.452
ProMoST 10.087
Dawson 10.599
Bjellqvist 10.262
Wikipedia 10.76
Rodwell 11.038
Grimsley 10.643
Solomon 10.628
Lehninger 10.599
Nozaki 10.467
DTASelect 10.248
Thurlkill 10.467
EMBOSS 10.833
Sillero 10.511
Patrickios 10.745
IPC_peptide 10.628
IPC2_peptide 9.326
IPC2.peptide.svr19 8.511
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2777
60
1968
462.8
52.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.734 ± 0.65
2.773 ± 0.325
4.861 ± 0.76
6.554 ± 0.968
5.51 ± 0.51
5.834 ± 0.276
2.125 ± 0.31
5.762 ± 1.034
6.338 ± 0.878
9.795 ± 1.013
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.909 ± 0.391
4.429 ± 0.992
4.501 ± 1.256
3.709 ± 0.253
5.474 ± 0.458
7.166 ± 0.393
5.329 ± 0.301
6.59 ± 0.739
1.044 ± 0.158
3.565 ± 0.214
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here