Phlox virus S

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Carlavirus

Average proteome isoelectric point is 7.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A4ZWC7|A4ZWC7_9VIRU Movement protein TGB2 OS=Phlox virus S OX=436066 PE=3 SV=1
MM1 pKa = 8.15DD2 pKa = 4.74ILVSLLCKK10 pKa = 10.7YY11 pKa = 10.47KK12 pKa = 10.69FEE14 pKa = 4.54RR15 pKa = 11.84LNSKK19 pKa = 10.13LCLPIIVNCVPGAGKK34 pKa = 10.18SSCIRR39 pKa = 11.84EE40 pKa = 4.48LISSDD45 pKa = 3.82SRR47 pKa = 11.84FCAYY51 pKa = 9.5TLGTEE56 pKa = 4.45DD57 pKa = 3.75PQNLRR62 pKa = 11.84GVRR65 pKa = 11.84IKK67 pKa = 10.74SFKK70 pKa = 11.18GEE72 pKa = 3.8IEE74 pKa = 3.8EE75 pKa = 4.85GKK77 pKa = 10.85FNVLDD82 pKa = 4.39EE83 pKa = 4.48YY84 pKa = 11.06TLSEE88 pKa = 4.03VDD90 pKa = 3.32LSKK93 pKa = 11.09FFVCFGDD100 pKa = 4.63PIQANSDD107 pKa = 3.53FARR110 pKa = 11.84PADD113 pKa = 4.72FICRR117 pKa = 11.84DD118 pKa = 3.19SKK120 pKa = 11.41RR121 pKa = 11.84FGKK124 pKa = 8.53CTATLLQNLGFDD136 pKa = 3.85IKK138 pKa = 11.23ACGEE142 pKa = 4.31DD143 pKa = 3.34SVQIAGIYY151 pKa = 10.26SEE153 pKa = 4.96DD154 pKa = 3.57PRR156 pKa = 11.84DD157 pKa = 3.23TVLYY161 pKa = 10.24HH162 pKa = 6.16EE163 pKa = 5.13EE164 pKa = 4.0EE165 pKa = 4.56VGCLLAKK172 pKa = 10.16HH173 pKa = 5.88CVEE176 pKa = 4.58AFHH179 pKa = 8.06ISEE182 pKa = 4.33VVGRR186 pKa = 11.84TFEE189 pKa = 4.18SVTFVTSHH197 pKa = 6.92AGVDD201 pKa = 3.66HH202 pKa = 7.83PDD204 pKa = 3.11RR205 pKa = 11.84AAAFQCLTRR214 pKa = 11.84HH215 pKa = 5.95RR216 pKa = 11.84KK217 pKa = 8.83SLLILCPDD225 pKa = 3.47ATYY228 pKa = 10.87GPPP231 pKa = 3.21

Molecular weight: 25.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A4ZWD0|A4ZWD0_9VIRU RNA silencing suppressor OS=Phlox virus S OX=436066 PE=3 SV=1
MM1 pKa = 7.69KK2 pKa = 10.18YY3 pKa = 9.01DD4 pKa = 3.68TVKK7 pKa = 10.98SEE9 pKa = 4.59IIFILLSKK17 pKa = 10.21CLEE20 pKa = 4.47RR21 pKa = 11.84GASAPLPIVCNIYY34 pKa = 9.52MRR36 pKa = 11.84AFNKK40 pKa = 10.16RR41 pKa = 11.84VGNGTSSYY49 pKa = 10.52ARR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84AASILRR60 pKa = 11.84CHH62 pKa = 6.39RR63 pKa = 11.84CYY65 pKa = 10.12RR66 pKa = 11.84VYY68 pKa = 10.75PPFWFTKK75 pKa = 8.7KK76 pKa = 10.42CNNRR80 pKa = 11.84TCVPGFKK87 pKa = 10.43YY88 pKa = 9.65NQKK91 pKa = 9.56VRR93 pKa = 11.84DD94 pKa = 4.54FILWGVTEE102 pKa = 4.88VIPHH106 pKa = 6.76PGYY109 pKa = 11.18NFF111 pKa = 3.42

Molecular weight: 13.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2802

64

1990

467.0

52.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.959 ± 0.418

3.034 ± 0.887

4.889 ± 0.602

7.031 ± 1.186

5.71 ± 0.495

6.21 ± 0.262

2.463 ± 0.356

4.889 ± 0.527

6.602 ± 0.886

9.493 ± 0.881

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.391 ± 0.505

4.604 ± 0.586

4.497 ± 0.87

2.891 ± 0.331

5.318 ± 0.785

6.638 ± 0.398

4.532 ± 0.578

7.209 ± 0.574

1.106 ± 0.201

3.533 ± 0.34
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski