Sclerotinia sclerotiorum negative-stranded RNA virus 4

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Mymonaviridae; Sclerotimonavirus; Drop sclerotimonavirus

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M4LD35|A0A0M4LD35_9MONO Gp1 OS=Sclerotinia sclerotiorum negative-stranded RNA virus 4 OX=1708391 PE=4 SV=1
MM1 pKa = 7.78ASKK4 pKa = 10.52QNPQVISEE12 pKa = 4.23EE13 pKa = 4.2AVASLDD19 pKa = 3.67NNVSGRR25 pKa = 11.84SFSMGVSKK33 pKa = 10.49MMSSLSRR40 pKa = 11.84ASWADD45 pKa = 3.33SVEE48 pKa = 4.08EE49 pKa = 4.44EE50 pKa = 4.81LPKK53 pKa = 11.04SPDD56 pKa = 3.05STAKK60 pKa = 9.42LTTIGSAKK68 pKa = 10.17LASTSPARR76 pKa = 11.84IDD78 pKa = 3.41SQSRR82 pKa = 11.84SDD84 pKa = 3.57APEE87 pKa = 4.12SEE89 pKa = 4.73SEE91 pKa = 5.01DD92 pKa = 3.56EE93 pKa = 4.43FQEE96 pKa = 4.32SPVIKK101 pKa = 10.65KK102 pKa = 10.05EE103 pKa = 3.94GSQLAAEE110 pKa = 4.18EE111 pKa = 4.79SEE113 pKa = 5.14VEE115 pKa = 4.38FNPGPVVKK123 pKa = 10.69GSAPKK128 pKa = 10.0QAVGILGVRR137 pKa = 11.84GNQRR141 pKa = 11.84EE142 pKa = 4.23ILNPTAQKK150 pKa = 8.98ITDD153 pKa = 3.69EE154 pKa = 4.14QLAGTTMKK162 pKa = 9.25ITLDD166 pKa = 3.22KK167 pKa = 10.44LQQVVEE173 pKa = 4.24AQDD176 pKa = 3.57NVIKK180 pKa = 10.7ILLQRR185 pKa = 11.84LDD187 pKa = 3.65ASDD190 pKa = 3.55TKK192 pKa = 10.61IANLLATINSLKK204 pKa = 10.49VVAEE208 pKa = 4.31DD209 pKa = 3.23VSSRR213 pKa = 11.84VKK215 pKa = 10.92SIDD218 pKa = 3.41ADD220 pKa = 3.7ALDD223 pKa = 4.65VLNQAAKK230 pKa = 9.67IVRR233 pKa = 11.84DD234 pKa = 3.58NRR236 pKa = 11.84VPDD239 pKa = 3.61APIAKK244 pKa = 9.25IEE246 pKa = 4.02QEE248 pKa = 4.12LEE250 pKa = 3.88RR251 pKa = 11.84TQDD254 pKa = 3.46SVAKK258 pKa = 9.57AAEE261 pKa = 4.1RR262 pKa = 11.84APIQSPKK269 pKa = 10.11IKK271 pKa = 9.95ASKK274 pKa = 9.03GARR277 pKa = 11.84RR278 pKa = 11.84LKK280 pKa = 10.87LSFEE284 pKa = 4.37

Molecular weight: 30.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M4KRD1|A0A0M4KRD1_9MONO Gp2 OS=Sclerotinia sclerotiorum negative-stranded RNA virus 4 OX=1708391 PE=4 SV=1
MM1 pKa = 7.37SRR3 pKa = 11.84LRR5 pKa = 11.84LPQSFSGPVWTFSILGVIVSKK26 pKa = 10.78RR27 pKa = 11.84PEE29 pKa = 3.76ILLQTRR35 pKa = 11.84LAALRR40 pKa = 11.84TLILVSRR47 pKa = 11.84STSLSTRR54 pKa = 11.84VSMLRR59 pKa = 11.84LPIEE63 pKa = 4.4VIAPFSPEE71 pKa = 3.23MTAVHH76 pKa = 6.52EE77 pKa = 4.72FCEE80 pKa = 3.95QLPDD84 pKa = 4.61HH85 pKa = 6.93ISVSDD90 pKa = 3.85LNRR93 pKa = 11.84VIDD96 pKa = 3.66IGLAGLTVAPSTLATYY112 pKa = 10.21IAVASVSEE120 pKa = 4.19HH121 pKa = 6.49LSCEE125 pKa = 4.12AKK127 pKa = 10.73VLTASLGIGEE137 pKa = 4.83LPRR140 pKa = 11.84SRR142 pKa = 11.84RR143 pKa = 11.84RR144 pKa = 11.84EE145 pKa = 3.4IRR147 pKa = 11.84FEE149 pKa = 3.88VVRR152 pKa = 11.84ILNEE156 pKa = 3.78SDD158 pKa = 3.03VTLRR162 pKa = 11.84DD163 pKa = 3.51KK164 pKa = 11.47VKK166 pKa = 10.89LIQQDD171 pKa = 3.47ILSSSIPSATEE182 pKa = 3.37

Molecular weight: 20.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3015

158

2010

603.0

66.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.894 ± 1.319

1.194 ± 0.436

5.373 ± 0.468

5.904 ± 0.842

3.549 ± 0.537

5.771 ± 0.719

1.725 ± 0.4

6.103 ± 0.516

4.71 ± 0.794

10.912 ± 0.72

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.454 ± 0.145

3.018 ± 0.348

5.274 ± 0.412

4.046 ± 0.43

6.534 ± 0.648

9.983 ± 0.74

5.274 ± 0.409

6.866 ± 0.36

1.327 ± 0.382

2.09 ± 0.576
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski