Capybara microvirus Cap3_SP_441
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W5X1|A0A4P8W5X1_9VIRU Nonstructural protein OS=Capybara microvirus Cap3_SP_441 OX=2585453 PE=4 SV=1
MM1 pKa = 7.35 SRR3 pKa = 11.84 NEE5 pKa = 3.64 LAYY8 pKa = 10.03 EE9 pKa = 4.04 VLDD12 pKa = 4.63 TIDD15 pKa = 3.87 TPRR18 pKa = 11.84 STWQRR23 pKa = 11.84 NFTHH27 pKa = 5.98 KK28 pKa = 9.74 TSFNAGKK35 pKa = 10.27 LIPFYY40 pKa = 10.25 IDD42 pKa = 2.91 TDD44 pKa = 4.38 IIPGTTIKK52 pKa = 10.78 NKK54 pKa = 7.91 TSIVIRR60 pKa = 11.84 MSTPIAPVMDD70 pKa = 4.2 NLYY73 pKa = 10.83 LDD75 pKa = 4.06 TYY77 pKa = 10.16 YY78 pKa = 10.85 FKK80 pKa = 10.92 CSKK83 pKa = 9.39 FWYY86 pKa = 8.05 WEE88 pKa = 3.68 HH89 pKa = 6.45 FRR91 pKa = 11.84 AQMGEE96 pKa = 4.08 NNQSAWTQTIEE107 pKa = 4.47 YY108 pKa = 9.45 IEE110 pKa = 4.36 PQIKK114 pKa = 8.49 MLSMEE119 pKa = 4.1 YY120 pKa = 10.41 GANDD124 pKa = 3.34 VACYY128 pKa = 9.56 MGMPINVANLEE139 pKa = 4.18 WSKK142 pKa = 11.35 LPVQAYY148 pKa = 9.67 IDD150 pKa = 3.21 IWNNWFRR157 pKa = 11.84 DD158 pKa = 3.68 EE159 pKa = 4.63 NLQAPITLDD168 pKa = 3.21 KK169 pKa = 11.01 TDD171 pKa = 4.44 ADD173 pKa = 4.01 LTADD177 pKa = 3.42 ATINTGFGLLPVCKK191 pKa = 9.86 FHH193 pKa = 8.39 DD194 pKa = 4.49 YY195 pKa = 8.25 FTSLLPQPQKK205 pKa = 11.38 GEE207 pKa = 4.09 AVTMPLGVAAPVSGTVKK224 pKa = 10.85 GNGLQLGVTNGTTNFVPTTRR244 pKa = 11.84 GSNYY248 pKa = 10.22 ASNTFGDD255 pKa = 4.02 SLGSFSEE262 pKa = 4.34 FTPGGTKK269 pKa = 9.9 TYY271 pKa = 10.66 GVTPDD276 pKa = 3.36 EE277 pKa = 4.69 SKK279 pKa = 10.89 SGLVIGTAYY288 pKa = 10.71 ADD290 pKa = 3.79 LTSATAATFNALRR303 pKa = 11.84 LAGATQRR310 pKa = 11.84 ILEE313 pKa = 4.15 KK314 pKa = 10.39 DD315 pKa = 3.0 ARR317 pKa = 11.84 FGTRR321 pKa = 11.84 YY322 pKa = 10.0 PEE324 pKa = 4.04 FLKK327 pKa = 10.96 GQYY330 pKa = 10.34 GVTAADD336 pKa = 3.67 EE337 pKa = 4.4 CLLIPEE343 pKa = 4.23 YY344 pKa = 10.85 LGGKK348 pKa = 9.42 RR349 pKa = 11.84 IPINIEE355 pKa = 3.84 QVCQTSSTDD364 pKa = 3.15 STSPLGEE371 pKa = 4.06 TGAMSVTADD380 pKa = 3.13 INEE383 pKa = 4.73 DD384 pKa = 3.43 FTKK387 pKa = 10.84 SFTKK391 pKa = 10.11 HH392 pKa = 6.27 DD393 pKa = 3.95 FLIGVCCVRR402 pKa = 11.84 AEE404 pKa = 3.94 HH405 pKa = 6.96 TYY407 pKa = 9.94 QQGIPLQFKK416 pKa = 9.92 RR417 pKa = 11.84 KK418 pKa = 9.03 RR419 pKa = 11.84 RR420 pKa = 11.84 LDD422 pKa = 3.49 TYY424 pKa = 10.51 HH425 pKa = 7.55 PSLAHH430 pKa = 6.92 IGNQPVYY437 pKa = 10.03 NFEE440 pKa = 4.34 IFAQGNSTDD449 pKa = 4.19 DD450 pKa = 3.11 EE451 pKa = 4.97 VMGYY455 pKa = 9.92 KK456 pKa = 9.81 EE457 pKa = 3.85 AWQEE461 pKa = 3.68 YY462 pKa = 9.17 LYY464 pKa = 10.9 KK465 pKa = 10.26 PNRR468 pKa = 11.84 ISGQMLSTYY477 pKa = 7.29 TQSLDD482 pKa = 2.93 VWHH485 pKa = 6.74 YY486 pKa = 10.96 GDD488 pKa = 5.8 DD489 pKa = 4.11 YY490 pKa = 12.05 VNLPVLSSEE499 pKa = 4.47 WIVEE503 pKa = 3.72 PTEE506 pKa = 4.26 YY507 pKa = 9.51 IDD509 pKa = 3.24 RR510 pKa = 11.84 TLAVSSSVSNQFWADD525 pKa = 2.99 IYY527 pKa = 10.83 IEE529 pKa = 4.41 QIVSAPIPINRR540 pKa = 11.84 VPGLIDD546 pKa = 3.56 HH547 pKa = 6.51 YY548 pKa = 11.72
Molecular weight: 61.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.757
IPC2_protein 4.902
IPC_protein 4.863
Toseland 4.749
ProMoST 4.991
Dawson 4.838
Bjellqvist 4.978
Wikipedia 4.724
Rodwell 4.736
Grimsley 4.673
Solomon 4.838
Lehninger 4.8
Nozaki 4.952
DTASelect 5.13
Thurlkill 4.762
EMBOSS 4.749
Sillero 5.016
Patrickios 3.312
IPC_peptide 4.851
IPC2_peptide 5.003
IPC2.peptide.svr19 4.984
Protein with the highest isoelectric point:
>tr|A0A4P8W8K7|A0A4P8W8K7_9VIRU Replication initiator protein OS=Capybara microvirus Cap3_SP_441 OX=2585453 PE=4 SV=1
MM1 pKa = 7.81 HH2 pKa = 6.78 YY3 pKa = 10.63 NCRR6 pKa = 11.84 KK7 pKa = 10.19 NKK9 pKa = 9.67 GLQEE13 pKa = 4.04 QNSIMLSVGTNKK25 pKa = 10.22 SSGRR29 pKa = 11.84 VPTFFIKK36 pKa = 10.46 EE37 pKa = 4.06 SRR39 pKa = 11.84 EE40 pKa = 4.09 MVCLNPITAWKK51 pKa = 10.23 KK52 pKa = 10.41 KK53 pKa = 10.11 FITLGTNPQFYY64 pKa = 9.92 EE65 pKa = 4.09 YY66 pKa = 10.3 EE67 pKa = 3.91 KK68 pKa = 10.7 KK69 pKa = 10.28 IRR71 pKa = 11.84 FKK73 pKa = 11.33 NPNDD77 pKa = 3.38 EE78 pKa = 4.15 NFEE81 pKa = 4.24 QIQLPCGKK89 pKa = 10.1 CLGCRR94 pKa = 11.84 LDD96 pKa = 5.06 HH97 pKa = 6.61 ANAWATRR104 pKa = 11.84 IMLEE108 pKa = 4.43 AKK110 pKa = 9.95 NHH112 pKa = 5.5 EE113 pKa = 4.52 KK114 pKa = 10.79 NCFITLTYY122 pKa = 10.3 NPKK125 pKa = 10.11 NLPLTEE131 pKa = 4.15 TGKK134 pKa = 8.13 MTLKK138 pKa = 10.7 KK139 pKa = 10.44 SDD141 pKa = 2.96 IQKK144 pKa = 9.2 FFKK147 pKa = 10.53 RR148 pKa = 11.84 LRR150 pKa = 11.84 EE151 pKa = 3.95 KK152 pKa = 10.34 TKK154 pKa = 10.84 LKK156 pKa = 10.71 LSYY159 pKa = 9.68 FCCGEE164 pKa = 3.78 YY165 pKa = 10.73 GPRR168 pKa = 11.84 TFRR171 pKa = 11.84 PHH173 pKa = 3.87 YY174 pKa = 8.84 HH175 pKa = 6.92 AIIFGYY181 pKa = 10.14 EE182 pKa = 3.78 PSDD185 pKa = 3.33 LKK187 pKa = 11.24 LRR189 pKa = 11.84 AFSKK193 pKa = 9.71 TNQKK197 pKa = 9.07 MFEE200 pKa = 4.15 SEE202 pKa = 4.36 EE203 pKa = 3.97 LLKK206 pKa = 10.09 TWGLGFITIEE216 pKa = 3.75 EE217 pKa = 4.53 LNYY220 pKa = 9.19 KK221 pKa = 6.61 TACYY225 pKa = 7.13 TARR228 pKa = 11.84 YY229 pKa = 6.98 VQKK232 pKa = 10.82 KK233 pKa = 10.37 AGLKK237 pKa = 8.07 TNSRR241 pKa = 11.84 KK242 pKa = 8.33 FTGATEE248 pKa = 4.21 TKK250 pKa = 10.14 IKK252 pKa = 9.98 IDD254 pKa = 3.38 EE255 pKa = 4.35 RR256 pKa = 11.84 TGDD259 pKa = 3.79 EE260 pKa = 4.19 YY261 pKa = 11.63 LSTINKK267 pKa = 9.75 LKK269 pKa = 10.08 TEE271 pKa = 3.95 KK272 pKa = 10.17 FDD274 pKa = 3.3 NYY276 pKa = 10.37 GRR278 pKa = 11.84 EE279 pKa = 3.87 KK280 pKa = 10.93 EE281 pKa = 4.29 FIVMSKK287 pKa = 10.44 KK288 pKa = 9.99 PAIGLNYY295 pKa = 9.38 WLEE298 pKa = 4.15 NKK300 pKa = 9.98 EE301 pKa = 3.95 KK302 pKa = 10.32 ILRR305 pKa = 11.84 NKK307 pKa = 10.19 GILIKK312 pKa = 9.9 IDD314 pKa = 3.45 EE315 pKa = 4.53 TVKK318 pKa = 10.68 LKK320 pKa = 10.27 PIPRR324 pKa = 11.84 YY325 pKa = 8.98 FEE327 pKa = 5.41 KK328 pKa = 10.44 IMEE331 pKa = 4.45 RR332 pKa = 11.84 EE333 pKa = 3.98 NKK335 pKa = 7.57 EE336 pKa = 3.82 TLDD339 pKa = 3.65 RR340 pKa = 11.84 LKK342 pKa = 10.87 HH343 pKa = 4.97 EE344 pKa = 4.57 KK345 pKa = 10.36 KK346 pKa = 10.67 EE347 pKa = 3.97 NFIKK351 pKa = 10.67 NQEE354 pKa = 4.37 LIVKK358 pKa = 9.85 KK359 pKa = 10.32 IKK361 pKa = 10.94 NNFKK365 pKa = 10.66 LCEE368 pKa = 4.0 EE369 pKa = 4.3 LTNDD373 pKa = 4.32 EE374 pKa = 4.09 KK375 pKa = 10.91 TKK377 pKa = 10.38 IYY379 pKa = 11.14 NNYY382 pKa = 9.54 LNKK385 pKa = 10.46 NLTNRR390 pKa = 11.84 AKK392 pKa = 10.11 YY393 pKa = 10.2 LKK395 pKa = 10.17 RR396 pKa = 11.84 GQII399 pKa = 3.58
Molecular weight: 47.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.965
IPC2_protein 9.019
IPC_protein 8.873
Toseland 9.838
ProMoST 9.443
Dawson 10.014
Bjellqvist 9.663
Wikipedia 10.145
Rodwell 10.599
Grimsley 10.072
Solomon 10.028
Lehninger 9.999
Nozaki 9.882
DTASelect 9.633
Thurlkill 9.882
EMBOSS 10.233
Sillero 9.94
Patrickios 10.101
IPC_peptide 10.028
IPC2_peptide 8.2
IPC2.peptide.svr19 7.92
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1389
77
548
277.8
31.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.479 ± 1.306
1.368 ± 0.404
4.896 ± 1.011
7.631 ± 1.562
4.536 ± 0.376
6.551 ± 1.251
1.512 ± 0.246
6.695 ± 0.49
9.719 ± 2.68
7.415 ± 0.72
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.52 ± 0.391
6.911 ± 0.77
3.6 ± 0.925
3.6 ± 0.523
3.744 ± 0.533
5.688 ± 1.15
7.919 ± 0.928
3.384 ± 0.976
1.152 ± 0.423
4.68 ± 0.424
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here