Paenibacillus sp. HGF7

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5992 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F5LF17|F5LF17_9BACL Flp1_like domain-containing protein OS=Paenibacillus sp. HGF7 OX=944559 GN=HMPREF9413_0992 PE=4 SV=1
MM1 pKa = 7.7PFINRR6 pKa = 11.84YY7 pKa = 7.53STTTNGAITFTGNTLGLSKK26 pKa = 10.69QNNANAPEE34 pKa = 4.18TARR37 pKa = 11.84SIGTFTTVNTSLQDD51 pKa = 3.27DD52 pKa = 5.18TYY54 pKa = 11.42PLGTTSDD61 pKa = 3.34HH62 pKa = 6.71RR63 pKa = 11.84LNSSSAILVLPSQSTVLYY81 pKa = 10.97AEE83 pKa = 5.21LIWGGSCNLGGEE95 pKa = 4.52NVSSALNNTVSLTNSAGVFTVTPDD119 pKa = 3.16TAAPSVNTSHH129 pKa = 6.37YY130 pKa = 10.29FYY132 pKa = 11.13VRR134 pKa = 11.84SANVTSLVRR143 pKa = 11.84AGGGGTYY150 pKa = 9.53TVGKK154 pKa = 10.66VPGTQGDD161 pKa = 4.09SEE163 pKa = 4.34NSINHH168 pKa = 6.53AGWTLAVIYY177 pKa = 10.51QNATLPSRR185 pKa = 11.84NMTIFVGNEE194 pKa = 3.5RR195 pKa = 11.84MDD197 pKa = 3.89TPSSTNSAVVSGFSTPTSGPLSGRR221 pKa = 11.84LFVSAQEE228 pKa = 4.46GGSPLTGDD236 pKa = 3.69RR237 pKa = 11.84MQFGPTAADD246 pKa = 3.86LAPVYY251 pKa = 10.62GPNNHH256 pKa = 5.69VSTISCSQINNDD268 pKa = 4.26SGLLDD273 pKa = 3.54TTGTFVDD280 pKa = 3.77RR281 pKa = 11.84NYY283 pKa = 9.01PTGSVISGGRR293 pKa = 11.84QGWDD297 pKa = 3.05VTNVDD302 pKa = 3.89VSSKK306 pKa = 10.62LINSQTSANIRR317 pKa = 11.84GTTYY321 pKa = 11.28GDD323 pKa = 3.33VYY325 pKa = 10.66LINALALQVNINAPVISVTKK345 pKa = 10.15EE346 pKa = 4.0SNKK349 pKa = 6.64TTAYY353 pKa = 9.1INEE356 pKa = 4.81AITYY360 pKa = 9.16KK361 pKa = 9.65VTISNSGNATANTLTLTHH379 pKa = 5.87QQPAGTTFIAGSVTVNGTAQASANPYY405 pKa = 9.32TGISLGNLANSQSIVVQFQSRR426 pKa = 11.84VTSRR430 pKa = 11.84PASPAQFADD439 pKa = 3.43QATVTYY445 pKa = 9.99QFQSVTGGTVFNGTAASSVVTVAAGNYY472 pKa = 8.81PPIASDD478 pKa = 3.39VTGTIAEE485 pKa = 4.33DD486 pKa = 3.61TSFTGQIPASDD497 pKa = 4.26PEE499 pKa = 4.52SAVLSYY505 pKa = 11.2SVASLPAHH513 pKa = 5.91GTAALNANGSFTYY526 pKa = 10.72VPDD529 pKa = 3.96LNFNGTDD536 pKa = 3.43SFSVTVSDD544 pKa = 5.17GSGGTANSLVTIRR557 pKa = 11.84VTPVNDD563 pKa = 3.64PSAAQNVSFTTLEE576 pKa = 4.13DD577 pKa = 3.28TSLSGKK583 pKa = 8.1VTATDD588 pKa = 3.08VDD590 pKa = 4.18GDD592 pKa = 4.13SLTFSLAVPPAHH604 pKa = 5.78GTVVFGPTGVFKK616 pKa = 9.29YY617 pKa = 9.35TPSPNYY623 pKa = 10.3NGTDD627 pKa = 3.04GFTYY631 pKa = 10.35SVSDD635 pKa = 3.99GNGGTATASVTITVTPVNDD654 pKa = 3.76PPVTSNVNLTTAEE667 pKa = 4.1DD668 pKa = 4.26TPVTGAVPPTDD679 pKa = 3.13VDD681 pKa = 3.98GDD683 pKa = 4.02GLTYY687 pKa = 10.51TLASPVTNGSVTLNADD703 pKa = 3.13GTYY706 pKa = 10.41VYY708 pKa = 11.13APGLHH713 pKa = 6.68FNGTDD718 pKa = 3.22SFAVRR723 pKa = 11.84VSDD726 pKa = 3.81GKK728 pKa = 11.35GGTAVSNVTITVTPVNDD745 pKa = 3.7PPVASGGSVVTPEE758 pKa = 4.16DD759 pKa = 3.84TPVSGKK765 pKa = 10.28INASDD770 pKa = 4.0ADD772 pKa = 3.99GDD774 pKa = 4.14ALGYY778 pKa = 11.14ALGTAPLNGTVVLAADD794 pKa = 4.42GSYY797 pKa = 10.24TYY799 pKa = 10.69TPLANFNGTDD809 pKa = 3.35SFTVTVSDD817 pKa = 4.3GNGGAAISHH826 pKa = 6.02IVVTVTPVNDD836 pKa = 4.05LPAEE840 pKa = 3.77QDD842 pKa = 3.51KK843 pKa = 11.44NVVSPEE849 pKa = 3.89DD850 pKa = 3.81TPVSGRR856 pKa = 11.84VFASDD861 pKa = 3.14VDD863 pKa = 3.96GDD865 pKa = 4.35ALTFAPGAPPANGTVVVASDD885 pKa = 3.27GGYY888 pKa = 8.9TYY890 pKa = 10.79TPAPDD895 pKa = 3.63YY896 pKa = 11.31NGTDD900 pKa = 3.24SFTVTVSDD908 pKa = 4.36GNGGTDD914 pKa = 3.02SAAATVTVTPVNDD927 pKa = 3.81PPVTSDD933 pKa = 3.12VALTTTEE940 pKa = 4.02DD941 pKa = 3.85TPVSGLVAATDD952 pKa = 3.76ADD954 pKa = 4.4GDD956 pKa = 4.3SLTYY960 pKa = 9.01TLRR963 pKa = 11.84APASNGTVSLNPDD976 pKa = 3.11GTFTYY981 pKa = 10.71VPALNFNGNDD991 pKa = 3.2SFAVEE996 pKa = 4.29VSDD999 pKa = 4.56GRR1001 pKa = 11.84GGTAVSNVILTVTPVNDD1018 pKa = 3.67PPVVFDD1024 pKa = 3.81EE1025 pKa = 4.53QCFTTEE1031 pKa = 4.27DD1032 pKa = 3.92TPVTCQIIASDD1043 pKa = 3.91VDD1045 pKa = 3.82GDD1047 pKa = 3.99APTYY1051 pKa = 11.16ALGTPPVSGTAVVTADD1067 pKa = 3.13GTYY1070 pKa = 9.97TYY1072 pKa = 10.35TPNPDD1077 pKa = 3.24FYY1079 pKa = 11.17GGDD1082 pKa = 3.5SFTVTVSDD1090 pKa = 4.43GNGGTATEE1098 pKa = 4.21TATVNVAPVNDD1109 pKa = 4.06APVTADD1115 pKa = 4.28LVLTTPEE1122 pKa = 3.76DD1123 pKa = 3.67TAASGQIAATDD1134 pKa = 3.66ADD1136 pKa = 4.03GDD1138 pKa = 4.17ALTFTLRR1145 pKa = 11.84TQPTRR1150 pKa = 11.84GSVILNPDD1158 pKa = 3.23GTFKK1162 pKa = 10.24YY1163 pKa = 9.41TPIPDD1168 pKa = 3.8FNGTDD1173 pKa = 3.33SFTVLVDD1180 pKa = 3.7DD1181 pKa = 4.93SNGGTAVANVTVTVTPVNDD1200 pKa = 3.77PPVAADD1206 pKa = 3.55VADD1209 pKa = 3.97VTPEE1213 pKa = 3.83DD1214 pKa = 4.42TPVSGQIPATDD1225 pKa = 3.24VDD1227 pKa = 4.13GDD1229 pKa = 4.11ALTFALGTPPVNGTAVVTVAGGYY1252 pKa = 8.43TYY1254 pKa = 10.65TPNPDD1259 pKa = 3.37FNGTDD1264 pKa = 3.24SFTVTVSDD1272 pKa = 4.15GKK1274 pKa = 11.23GGTATLTVTVTVTPVNDD1291 pKa = 3.81APVTADD1297 pKa = 3.76LALPTPEE1304 pKa = 4.36DD1305 pKa = 3.85TPLAGAVTATDD1316 pKa = 3.64ADD1318 pKa = 4.21GDD1320 pKa = 4.13ALTYY1324 pKa = 11.14LLGTFPTHH1332 pKa = 5.24GTAAVAADD1340 pKa = 3.65GTFTYY1345 pKa = 10.09TPNADD1350 pKa = 3.4FNGIDD1355 pKa = 3.5SFTVTVHH1362 pKa = 7.03DD1363 pKa = 4.41GAGGTDD1369 pKa = 3.37SAVVTVTVVPVNDD1382 pKa = 3.75APILGTVNEE1391 pKa = 4.49TTVEE1395 pKa = 4.04DD1396 pKa = 3.95TVLNGQITAADD1407 pKa = 3.9PEE1409 pKa = 4.79GDD1411 pKa = 3.43PVTYY1415 pKa = 10.96ALAAQAANGTAAVNADD1431 pKa = 2.94GSYY1434 pKa = 10.25TYY1436 pKa = 10.47TPNLNFNGLDD1446 pKa = 3.64FFVVTASDD1454 pKa = 3.84GQGGVASAVVNITVTPVNDD1473 pKa = 3.88PPFAAPLALSTPEE1486 pKa = 3.82NTPVSGRR1493 pKa = 11.84ISAYY1497 pKa = 10.15DD1498 pKa = 3.48RR1499 pKa = 11.84EE1500 pKa = 4.47GDD1502 pKa = 4.16PIVHH1506 pKa = 6.2TVNTEE1511 pKa = 3.86PGNGTVILNADD1522 pKa = 3.42GSFIYY1527 pKa = 10.35TPAQDD1532 pKa = 4.39FNGTDD1537 pKa = 3.22GFAIRR1542 pKa = 11.84VSDD1545 pKa = 3.58DD1546 pKa = 3.02RR1547 pKa = 11.84GAGFVSDD1554 pKa = 3.55VTVTVLPVNDD1564 pKa = 4.07PPRR1567 pKa = 11.84VRR1569 pKa = 11.84NVSQSTFVNQTVSGSIDD1586 pKa = 3.8ADD1588 pKa = 4.51DD1589 pKa = 4.54IDD1591 pKa = 5.53NDD1593 pKa = 3.68VLTYY1597 pKa = 11.13SLDD1600 pKa = 3.65TPPANGTATAGPGNTYY1616 pKa = 9.91TYY1618 pKa = 10.87VPNPTFTGTDD1628 pKa = 3.23SFQVRR1633 pKa = 11.84VTDD1636 pKa = 3.77EE1637 pKa = 4.3SGASAMAAITVEE1649 pKa = 3.99VSPVFTGSSIEE1660 pKa = 4.23TTLMTLQNTPLTSIVPLVPEE1680 pKa = 4.2VSAVYY1685 pKa = 10.29RR1686 pKa = 11.84LSTPPSNGSVSVNADD1701 pKa = 3.01RR1702 pKa = 11.84SFTYY1706 pKa = 10.56VPNQNFTGSDD1716 pKa = 3.46SFVLTEE1722 pKa = 4.27SGTLQTVLVKK1732 pKa = 10.47VVVLPANQAPLVGNVQSSTTRR1753 pKa = 11.84NAPVSGTITATDD1765 pKa = 3.95PEE1767 pKa = 4.73GQSLFYY1773 pKa = 11.02NLNTLPLNGTASVRR1787 pKa = 11.84TDD1789 pKa = 2.93GTFTYY1794 pKa = 10.04TPSRR1798 pKa = 11.84NFAGTDD1804 pKa = 3.17SFTVGVTDD1812 pKa = 3.54AGGAVSIALIVITVEE1827 pKa = 4.12RR1828 pKa = 11.84KK1829 pKa = 9.97DD1830 pKa = 4.03DD1831 pKa = 3.81GVGNAEE1837 pKa = 4.45PVPDD1841 pKa = 3.75TLTARR1846 pKa = 11.84SEE1848 pKa = 4.34TLL1850 pKa = 3.12

Molecular weight: 187.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F5LHH7|F5LHH7_9BACL Uncharacterized protein OS=Paenibacillus sp. HGF7 OX=944559 GN=HMPREF9413_5968 PE=4 SV=1
MM1 pKa = 7.88RR2 pKa = 11.84PTFNPNTRR10 pKa = 11.84TRR12 pKa = 11.84KK13 pKa = 9.15KK14 pKa = 9.4NHH16 pKa = 5.45GFRR19 pKa = 11.84KK20 pKa = 9.98RR21 pKa = 11.84MSTKK25 pKa = 10.0NGRR28 pKa = 11.84KK29 pKa = 8.73VLQARR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.17GRR39 pKa = 11.84KK40 pKa = 8.56VLSAA44 pKa = 4.05

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5992

0

5992

1790891

24

6152

298.9

33.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.827 ± 0.044

0.758 ± 0.011

5.051 ± 0.027

6.821 ± 0.044

4.128 ± 0.025

7.786 ± 0.037

2.021 ± 0.016

6.115 ± 0.034

5.387 ± 0.035

10.252 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.648 ± 0.018

3.524 ± 0.024

4.335 ± 0.025

3.59 ± 0.02

5.395 ± 0.034

6.248 ± 0.031

5.456 ± 0.032

7.072 ± 0.027

1.208 ± 0.012

3.378 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski