Corynebacterium durum F0235
Average proteome isoelectric point is 6.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2823 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|L1MGI6|L1MGI6_9CORY Carbamoyl-phosphate synthase large chain OS=Corynebacterium durum F0235 OX=1035195 GN=carB PE=3 SV=1
MM1 pKa = 6.85 PTVVSEE7 pKa = 3.98 YY8 pKa = 10.95 LAILRR13 pKa = 11.84 KK14 pKa = 9.97 NFNEE18 pKa = 4.05 DD19 pKa = 3.18 DD20 pKa = 4.06 RR21 pKa = 11.84 EE22 pKa = 4.37 SFAHH26 pKa = 5.31 VTGATDD32 pKa = 3.24 NDD34 pKa = 3.99 LARR37 pKa = 11.84 VLVVYY42 pKa = 10.15 PDD44 pKa = 3.65 TPEE47 pKa = 4.42 EE48 pKa = 3.93 FQQLLKK54 pKa = 10.78 QVDD57 pKa = 4.34 GTWGRR62 pKa = 11.84 DD63 pKa = 3.56 YY64 pKa = 11.53 GNHH67 pKa = 5.53 EE68 pKa = 4.12 VLVYY72 pKa = 9.22 MFASDD77 pKa = 4.18 LGAEE81 pKa = 4.18 EE82 pKa = 5.1 TEE84 pKa = 4.39 TGDD87 pKa = 3.04 YY88 pKa = 10.54 DD89 pKa = 3.67 YY90 pKa = 10.67 PYY92 pKa = 11.05 FMNSCEE98 pKa = 4.16 QILDD102 pKa = 3.67 EE103 pKa = 5.82 RR104 pKa = 11.84 DD105 pKa = 3.34 DD106 pKa = 4.1 SSIVDD111 pKa = 3.75 MYY113 pKa = 11.46 GDD115 pKa = 4.2 DD116 pKa = 4.95 AIEE119 pKa = 4.75 DD120 pKa = 3.77 SDD122 pKa = 4.23 VDD124 pKa = 3.48 VDD126 pKa = 3.85 PRR128 pKa = 11.84 IDD130 pKa = 3.39 PTIPMSQRR138 pKa = 11.84 LQFANCMNNGGTSQLYY154 pKa = 10.02 IDD156 pKa = 5.16 FNPTSRR162 pKa = 11.84 GVSGQIICYY171 pKa = 9.97 VHH173 pKa = 7.36 DD174 pKa = 4.84 PDD176 pKa = 3.69 AWIVLTPSFSDD187 pKa = 3.82 FLQKK191 pKa = 10.73 HH192 pKa = 6.13 IDD194 pKa = 3.7 SSCSFLPYY202 pKa = 10.2 NYY204 pKa = 10.23 EE205 pKa = 4.13 LDD207 pKa = 3.8 EE208 pKa = 5.74 SDD210 pKa = 3.48
Molecular weight: 23.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.74
IPC2_protein 3.757
IPC_protein 3.77
Toseland 3.541
ProMoST 3.91
Dawson 3.77
Bjellqvist 3.948
Wikipedia 3.719
Rodwell 3.592
Grimsley 3.452
Solomon 3.77
Lehninger 3.719
Nozaki 3.884
DTASelect 4.151
Thurlkill 3.605
EMBOSS 3.732
Sillero 3.897
Patrickios 0.604
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.81
Protein with the highest isoelectric point:
>tr|L1MD08|L1MD08_9CORY Bacterial transferase hexapeptide repeat protein OS=Corynebacterium durum F0235 OX=1035195 GN=HMPREF9997_02136 PE=4 SV=1
MM1 pKa = 7.63 LSLNRR6 pKa = 11.84 PSLRR10 pKa = 11.84 QLRR13 pKa = 11.84 NQQRR17 pKa = 11.84 QKK19 pKa = 11.33 SLLQHH24 pKa = 6.25 RR25 pKa = 11.84 RR26 pKa = 11.84 SQRR29 pKa = 11.84 TILQRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 SLPRR40 pKa = 11.84 NHH42 pKa = 7.1 RR43 pKa = 11.84 LHH45 pKa = 7.22 RR46 pKa = 11.84 GWQKK50 pKa = 10.75 NRR52 pKa = 11.84 LLLQRR57 pKa = 11.84 RR58 pKa = 11.84 LRR60 pKa = 11.84 SLRR63 pKa = 11.84 MTQQRR68 pKa = 11.84 QKK70 pKa = 10.34 NRR72 pKa = 11.84 QMSHH76 pKa = 6.52 LNWW79 pKa = 4.0
Molecular weight: 10.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.506
IPC_protein 13.115
Toseland 13.276
ProMoST 13.773
Dawson 13.276
Bjellqvist 13.276
Wikipedia 13.759
Rodwell 12.837
Grimsley 13.32
Solomon 13.773
Lehninger 13.685
Nozaki 13.276
DTASelect 13.276
Thurlkill 13.276
EMBOSS 13.773
Sillero 13.276
Patrickios 12.559
IPC_peptide 13.788
IPC2_peptide 12.778
IPC2.peptide.svr19 9.392
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2823
0
2823
843542
39
4655
298.8
32.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.059 ± 0.067
0.87 ± 0.013
6.156 ± 0.041
5.712 ± 0.045
3.358 ± 0.03
8.053 ± 0.05
2.343 ± 0.027
5.246 ± 0.041
3.201 ± 0.045
9.579 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.339 ± 0.022
3.099 ± 0.032
5.049 ± 0.038
3.443 ± 0.029
6.043 ± 0.042
6.049 ± 0.035
6.336 ± 0.045
8.321 ± 0.045
1.417 ± 0.017
2.328 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here