Cryptococcus gattii serotype B (strain R265) (Filobasidiella gattii) (Cryptococcus bacillisporus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes; Tremellales; Cryptococcaceae; Cryptococcus; Cryptococcus gattii species complex; Cryptococcus gattii VGII

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2942 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A095CAX8|A0A095CAX8_CRYGR Selenoprotein O OS=Cryptococcus gattii serotype B (strain R265) OX=294750 GN=CNBG_2597 PE=3 SV=1
MM1 pKa = 7.68IPIRR5 pKa = 11.84QTIPFGLLSCLALLGLVKK23 pKa = 10.66ADD25 pKa = 3.27GSTSNTSWPEE35 pKa = 3.58WVTNDD40 pKa = 3.3YY41 pKa = 11.4DD42 pKa = 4.31CVIGCMSGFNDD53 pKa = 4.73TITYY57 pKa = 9.86IPQDD61 pKa = 3.6ALAQQAFDD69 pKa = 4.54CTSSTCKK76 pKa = 10.64GDD78 pKa = 3.29VTGNYY83 pKa = 9.07YY84 pKa = 8.29QTLYY88 pKa = 10.8YY89 pKa = 9.78IQLFYY94 pKa = 10.83ATGSIYY100 pKa = 10.17EE101 pKa = 4.32YY102 pKa = 10.86SDD104 pKa = 3.6SAPDD108 pKa = 4.26GYY110 pKa = 11.29KK111 pKa = 10.14HH112 pKa = 7.21ANFTDD117 pKa = 3.96STGTTSDD124 pKa = 3.55AAVSGQSGDD133 pKa = 4.64DD134 pKa = 3.59NTIDD138 pKa = 3.51PTDD141 pKa = 3.3TSEE144 pKa = 5.39ADD146 pKa = 3.32FASATDD152 pKa = 4.12GVKK155 pKa = 7.93TTGSADD161 pKa = 3.29GGNDD165 pKa = 3.41GSSTDD170 pKa = 3.56TAAVAVDD177 pKa = 3.53TGRR180 pKa = 11.84EE181 pKa = 3.97NNGAAVKK188 pKa = 10.46ASEE191 pKa = 4.56PDD193 pKa = 3.78SIISGSEE200 pKa = 3.73PFSTKK205 pKa = 9.17STSSHH210 pKa = 6.17TDD212 pKa = 2.83TSIASSSVAADD223 pKa = 3.33ISDD226 pKa = 3.4SGAFKK231 pKa = 10.61IIGGGVWQSVLGALGALVLGGVWVGMM257 pKa = 4.33

Molecular weight: 26.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A095C319|A0A095C319_CRYGR Calcineurin-binding protein OS=Cryptococcus gattii serotype B (strain R265) OX=294750 GN=CNBG_1133 PE=3 SV=1
MM1 pKa = 7.43SPASPKK7 pKa = 9.93QPHH10 pKa = 6.4SPTTPPSPTSSPTHH24 pKa = 5.89SILRR28 pKa = 11.84RR29 pKa = 11.84TASTLSAPSHH39 pKa = 5.19SHH41 pKa = 5.76KK42 pKa = 10.96KK43 pKa = 8.34MLRR46 pKa = 11.84FTQVHH51 pKa = 6.56RR52 pKa = 11.84DD53 pKa = 3.23SDD55 pKa = 4.24TLLSTVGHH63 pKa = 6.29NEE65 pKa = 3.45DD66 pKa = 3.58VYY68 pKa = 9.68YY69 pKa = 10.23QGRR72 pKa = 11.84TVEE75 pKa = 4.18EE76 pKa = 3.93GLYY79 pKa = 9.78TDD81 pKa = 5.23RR82 pKa = 11.84LATDD86 pKa = 3.6RR87 pKa = 11.84RR88 pKa = 11.84RR89 pKa = 11.84YY90 pKa = 8.56HH91 pKa = 6.31QSDD94 pKa = 3.46PGTPNFQEE102 pKa = 3.92TADD105 pKa = 3.7QIRR108 pKa = 11.84RR109 pKa = 11.84TLSLASVDD117 pKa = 4.1SLLLLSVNQSEE128 pKa = 4.47RR129 pKa = 11.84TKK131 pKa = 10.93FLAEE135 pKa = 3.7VSRR138 pKa = 11.84AGRR141 pKa = 11.84GLVWRR146 pKa = 11.84DD147 pKa = 3.18EE148 pKa = 4.45SEE150 pKa = 4.23QCLLPQDD157 pKa = 3.74NEE159 pKa = 4.33RR160 pKa = 11.84AVVLALKK167 pKa = 10.4RR168 pKa = 11.84GLRR171 pKa = 11.84SFILAFSVRR180 pKa = 11.84SGINVLLLLFRR191 pKa = 11.84LLRR194 pKa = 11.84KK195 pKa = 9.05HH196 pKa = 6.22APKK199 pKa = 10.65LSLPLLRR206 pKa = 11.84HH207 pKa = 6.6AIFGPEE213 pKa = 3.92PFRR216 pKa = 11.84FGGMIGTFTLLYY228 pKa = 10.32TFTLHH233 pKa = 7.65LLRR236 pKa = 11.84LAPPLSYY243 pKa = 10.16FRR245 pKa = 11.84RR246 pKa = 11.84RR247 pKa = 11.84VRR249 pKa = 11.84AGLWNKK255 pKa = 7.25TTFGPPEE262 pKa = 3.88RR263 pKa = 11.84EE264 pKa = 4.07GNEE267 pKa = 4.16GEE269 pKa = 4.55RR270 pKa = 11.84RR271 pKa = 11.84WQAAVAGAVGSLGLLWEE288 pKa = 4.88GKK290 pKa = 7.45SRR292 pKa = 11.84RR293 pKa = 11.84RR294 pKa = 11.84GVAQQMFVRR303 pKa = 11.84GLQASYY309 pKa = 10.87NQYY312 pKa = 8.55TPRR315 pKa = 11.84FGIHH319 pKa = 6.45IPHH322 pKa = 7.38GDD324 pKa = 3.57LLLFGACCGQIMFAFLMSPEE344 pKa = 4.79TIPKK348 pKa = 9.8EE349 pKa = 3.94YY350 pKa = 10.98NNWILAASRR359 pKa = 11.84VPSFAILANRR369 pKa = 11.84TQVRR373 pKa = 11.84QGYY376 pKa = 8.33IQDD379 pKa = 4.02ALVQEE384 pKa = 4.14ALNYY388 pKa = 10.36KK389 pKa = 10.28GITAINRR396 pKa = 11.84RR397 pKa = 11.84RR398 pKa = 11.84LEE400 pKa = 3.86NVLQKK405 pKa = 10.25IRR407 pKa = 11.84GGQQPDD413 pKa = 3.45AVPCGMVHH421 pKa = 6.64PWCNSCTEE429 pKa = 4.14TNFRR433 pKa = 11.84RR434 pKa = 11.84FFAVCRR440 pKa = 11.84FMLPVYY446 pKa = 10.18SALHH450 pKa = 6.75LIPMLVLRR458 pKa = 11.84RR459 pKa = 11.84HH460 pKa = 5.03HH461 pKa = 6.36VKK463 pKa = 10.27RR464 pKa = 11.84DD465 pKa = 3.6PVRR468 pKa = 11.84MMARR472 pKa = 11.84ALWGIGRR479 pKa = 11.84SCSFLGVFVVIYY491 pKa = 10.02QVLFCARR498 pKa = 11.84VQALEE503 pKa = 4.07KK504 pKa = 10.94SRR506 pKa = 11.84GSDD509 pKa = 4.35FIRR512 pKa = 11.84KK513 pKa = 8.46LLKK516 pKa = 10.17RR517 pKa = 11.84KK518 pKa = 8.95EE519 pKa = 4.22VFWALGFTTCLSLLVEE535 pKa = 4.29EE536 pKa = 4.78KK537 pKa = 10.45KK538 pKa = 10.69RR539 pKa = 11.84RR540 pKa = 11.84AEE542 pKa = 3.54LAMYY546 pKa = 8.07VLPRR550 pKa = 11.84ALEE553 pKa = 4.33SAWSGARR560 pKa = 11.84KK561 pKa = 9.28RR562 pKa = 11.84AWVPLVPFGEE572 pKa = 4.74TILGAAAMAMVMDD585 pKa = 5.41AYY587 pKa = 9.89KK588 pKa = 10.08HH589 pKa = 5.81QPDD592 pKa = 3.71AMSGIVRR599 pKa = 11.84RR600 pKa = 11.84LLFQLVGPGG609 pKa = 3.09

Molecular weight: 68.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2942

0

2942

1331672

38

2365

452.6

49.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.515 ± 0.042

1.0 ± 0.016

5.322 ± 0.028

6.754 ± 0.043

3.43 ± 0.029

7.186 ± 0.038

2.302 ± 0.019

4.888 ± 0.032

5.422 ± 0.04

8.964 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.148 ± 0.017

3.377 ± 0.021

6.458 ± 0.053

3.766 ± 0.036

5.848 ± 0.034

8.795 ± 0.064

5.745 ± 0.026

6.157 ± 0.03

1.362 ± 0.016

2.56 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski