Avon-Heathcote Estuary associated circular virus 14

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 7.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5IMG6|A0A0C5IMG6_9CIRC ATP-dependent helicase Rep OS=Avon-Heathcote Estuary associated circular virus 14 OX=1618237 PE=3 SV=1
MM1 pKa = 7.76SGRR4 pKa = 11.84NWCFTINNPEE14 pKa = 3.94LTDD17 pKa = 4.26YY18 pKa = 10.95PEE20 pKa = 3.98QWPVTHH26 pKa = 7.33LKK28 pKa = 9.61TILYY32 pKa = 7.71QVEE35 pKa = 4.17MGEE38 pKa = 4.31EE39 pKa = 4.25EE40 pKa = 4.29TLHH43 pKa = 5.47LQGYY47 pKa = 8.95LEE49 pKa = 4.48IDD51 pKa = 3.57SPRR54 pKa = 11.84AIGWLKK60 pKa = 10.23GLNGRR65 pKa = 11.84AHH67 pKa = 6.06WEE69 pKa = 3.81RR70 pKa = 11.84RR71 pKa = 11.84KK72 pKa = 9.19GTRR75 pKa = 11.84SQAILYY81 pKa = 8.25CCKK84 pKa = 9.99EE85 pKa = 4.03DD86 pKa = 3.63SRR88 pKa = 11.84LVRR91 pKa = 11.84PRR93 pKa = 11.84LWTSRR98 pKa = 11.84DD99 pKa = 3.77LSWEE103 pKa = 4.0DD104 pKa = 4.62LDD106 pKa = 4.85PDD108 pKa = 4.87SLTSCLNSRR117 pKa = 11.84GITLATSEE125 pKa = 4.55NGNCGTNSRR134 pKa = 11.84LLEE137 pKa = 4.32IKK139 pKa = 10.06EE140 pKa = 4.12KK141 pKa = 10.67LSEE144 pKa = 4.01RR145 pKa = 11.84SSSIEE150 pKa = 3.96EE151 pKa = 3.91IADD154 pKa = 3.3EE155 pKa = 5.83HH156 pKa = 6.33FDD158 pKa = 3.08LWVRR162 pKa = 11.84HH163 pKa = 4.51YY164 pKa = 10.83RR165 pKa = 11.84AFEE168 pKa = 3.87KK169 pKa = 10.93YY170 pKa = 7.1MTMKK174 pKa = 10.78AEE176 pKa = 3.92PRR178 pKa = 11.84NWEE181 pKa = 4.24CEE183 pKa = 3.98VHH185 pKa = 6.37VLYY188 pKa = 11.02GPTGTGKK195 pKa = 10.57SKK197 pKa = 10.04WAMDD201 pKa = 4.67EE202 pKa = 4.16YY203 pKa = 10.71PGAYY207 pKa = 7.89WKK209 pKa = 10.09QRR211 pKa = 11.84SKK213 pKa = 9.66WWDD216 pKa = 3.52GYY218 pKa = 10.73VGHH221 pKa = 6.3EE222 pKa = 3.95TVIIDD227 pKa = 4.73EE228 pKa = 4.61YY229 pKa = 11.22YY230 pKa = 10.33GWLPFDD236 pKa = 4.48LLLRR240 pKa = 11.84LCDD243 pKa = 4.06RR244 pKa = 11.84YY245 pKa = 10.36PLLVEE250 pKa = 4.4SKK252 pKa = 10.39GGQLQFVAKK261 pKa = 9.59TIIFTTNKK269 pKa = 10.19CPDD272 pKa = 2.59QWYY275 pKa = 10.35NSDD278 pKa = 3.12SCYY281 pKa = 10.45FPAFEE286 pKa = 4.06RR287 pKa = 11.84RR288 pKa = 11.84VDD290 pKa = 3.03KK291 pKa = 10.34WHH293 pKa = 6.34YY294 pKa = 9.77LPSLGFHH301 pKa = 6.64SRR303 pKa = 11.84FTRR306 pKa = 11.84FKK308 pKa = 9.4EE309 pKa = 4.4FKK311 pKa = 10.39DD312 pKa = 3.62VTSS315 pKa = 3.63

Molecular weight: 37.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5IMG6|A0A0C5IMG6_9CIRC ATP-dependent helicase Rep OS=Avon-Heathcote Estuary associated circular virus 14 OX=1618237 PE=3 SV=1
MM1 pKa = 7.98RR2 pKa = 11.84GRR4 pKa = 11.84KK5 pKa = 8.65RR6 pKa = 11.84DD7 pKa = 3.2HH8 pKa = 6.7RR9 pKa = 11.84GRR11 pKa = 11.84FIRR14 pKa = 11.84ATVVGAGRR22 pKa = 11.84AIRR25 pKa = 11.84YY26 pKa = 7.33AAGVGSGFAGLASSFARR43 pKa = 11.84TRR45 pKa = 11.84RR46 pKa = 11.84NRR48 pKa = 11.84MTSGQGVTFEE58 pKa = 4.13NDD60 pKa = 2.35RR61 pKa = 11.84TRR63 pKa = 11.84IYY65 pKa = 10.24RR66 pKa = 11.84RR67 pKa = 11.84KK68 pKa = 9.5RR69 pKa = 11.84APARR73 pKa = 11.84KK74 pKa = 8.29RR75 pKa = 11.84RR76 pKa = 11.84RR77 pKa = 11.84WRR79 pKa = 11.84KK80 pKa = 8.57ILRR83 pKa = 11.84VNNAVDD89 pKa = 3.52QTKK92 pKa = 10.77LGTRR96 pKa = 11.84TILFNKK102 pKa = 8.77SQTYY106 pKa = 10.14GNSTAGQHH114 pKa = 4.94GLAWAALYY122 pKa = 9.36GANSANSVLNDD133 pKa = 4.22LRR135 pKa = 11.84AIWSNEE141 pKa = 3.32ALNQNPTAGGGPTSYY156 pKa = 11.2NSTKK160 pKa = 10.01MFFKK164 pKa = 10.75SAVLDD169 pKa = 3.43MTIRR173 pKa = 11.84NVSSVGDD180 pKa = 4.98DD181 pKa = 3.08ITSPADD187 pKa = 3.27VPLEE191 pKa = 3.74LDD193 pKa = 3.21IYY195 pKa = 11.0EE196 pKa = 4.35MSSKK200 pKa = 10.48CRR202 pKa = 11.84MVEE205 pKa = 3.7NDD207 pKa = 3.83AGTALKK213 pKa = 9.84QWNNIPEE220 pKa = 4.4TFDD223 pKa = 3.32EE224 pKa = 4.73ALDD227 pKa = 3.51NTFAIDD233 pKa = 4.35GAGTKK238 pKa = 10.1CDD240 pKa = 3.72LNLRR244 pKa = 11.84GVTPWEE250 pKa = 3.79NTFALSRR257 pKa = 11.84YY258 pKa = 8.89KK259 pKa = 10.94YY260 pKa = 10.07KK261 pKa = 10.17IHH263 pKa = 7.24KK264 pKa = 6.91KK265 pKa = 6.96TKK267 pKa = 8.48YY268 pKa = 10.06RR269 pKa = 11.84IGRR272 pKa = 11.84GNSITYY278 pKa = 7.76QFRR281 pKa = 11.84DD282 pKa = 3.53PKK284 pKa = 11.12NRR286 pKa = 11.84TTSHH290 pKa = 6.39NALGDD295 pKa = 3.49LAGFNKK301 pKa = 10.32PGWTHH306 pKa = 6.26NLLIVYY312 pKa = 9.55KK313 pKa = 9.7SVPGYY318 pKa = 10.69SVGTAPTDD326 pKa = 3.12IQEE329 pKa = 4.54RR330 pKa = 11.84IEE332 pKa = 3.66IGYY335 pKa = 6.38TRR337 pKa = 11.84KK338 pKa = 8.48YY339 pKa = 7.3TYY341 pKa = 10.18KK342 pKa = 10.6VEE344 pKa = 4.15GHH346 pKa = 5.9SDD348 pKa = 3.18ARR350 pKa = 11.84SLYY353 pKa = 10.16INSS356 pKa = 3.54

Molecular weight: 39.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

671

315

356

335.5

38.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.408 ± 1.876

1.639 ± 0.783

5.365 ± 0.225

5.663 ± 1.871

3.577 ± 0.15

7.75 ± 0.901

2.235 ± 0.4

4.769 ± 0.413

5.812 ± 0.063

7.899 ± 1.25

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.639 ± 0.033

5.514 ± 1.301

3.726 ± 0.462

2.385 ± 0.1

8.793 ± 0.959

7.154 ± 0.095

7.452 ± 0.709

4.322 ± 0.33

3.13 ± 1.05

4.769 ± 0.2
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski