Farfantepenaeus duorarum pink shrimp associated circular virus
Average proteome isoelectric point is 7.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0K1RLN2|A0A0K1RLN2_9CIRC Putative replication initiation protein OS=Farfantepenaeus duorarum pink shrimp associated circular virus OX=1692248 PE=4 SV=1
MM1 pKa = 7.26 AQAKK5 pKa = 9.57 RR6 pKa = 11.84 WVFTLNNYY14 pKa = 9.94 SGADD18 pKa = 3.62 EE19 pKa = 4.12 QLLEE23 pKa = 3.99 QLSRR27 pKa = 11.84 STDD30 pKa = 3.48 VVTLIYY36 pKa = 10.42 GRR38 pKa = 11.84 EE39 pKa = 3.98 VAPGTGTNHH48 pKa = 5.72 LQGMIIFATRR58 pKa = 11.84 KK59 pKa = 9.14 RR60 pKa = 11.84 LRR62 pKa = 11.84 QIRR65 pKa = 11.84 NYY67 pKa = 10.49 PPFQRR72 pKa = 11.84 AHH74 pKa = 6.55 LEE76 pKa = 4.29 VMRR79 pKa = 11.84 STPARR84 pKa = 11.84 ARR86 pKa = 11.84 EE87 pKa = 3.92 YY88 pKa = 10.55 CIKK91 pKa = 11.0 DD92 pKa = 3.13 GDD94 pKa = 3.93 FVEE97 pKa = 4.77 YY98 pKa = 10.88 GEE100 pKa = 4.21 PVEE103 pKa = 4.45 EE104 pKa = 3.99 NRR106 pKa = 11.84 QGRR109 pKa = 11.84 RR110 pKa = 11.84 NDD112 pKa = 2.8 IHH114 pKa = 8.24 DD115 pKa = 4.41 IIAWLDD121 pKa = 3.46 LFIEE125 pKa = 4.55 EE126 pKa = 4.39 NNRR129 pKa = 11.84 PPTQEE134 pKa = 4.09 EE135 pKa = 4.68 VAQLQPIAMLRR146 pKa = 11.84 YY147 pKa = 9.67 RR148 pKa = 11.84 NFMDD152 pKa = 4.89 LARR155 pKa = 11.84 LRR157 pKa = 11.84 APQPQLIRR165 pKa = 11.84 NGVARR170 pKa = 11.84 EE171 pKa = 4.08 GWQMDD176 pKa = 4.26 LEE178 pKa = 4.57 HH179 pKa = 7.54 ALDD182 pKa = 4.3 GDD184 pKa = 3.73 APDD187 pKa = 3.48 RR188 pKa = 11.84 HH189 pKa = 6.94 ILFYY193 pKa = 11.02 VDD195 pKa = 3.33 PAGGNGKK202 pKa = 7.29 TWFQQYY208 pKa = 11.21 LLTKK212 pKa = 8.89 YY213 pKa = 9.63 PRR215 pKa = 11.84 RR216 pKa = 11.84 VQILGTGNFADD227 pKa = 3.98 CAYY230 pKa = 10.54 AVDD233 pKa = 3.9 EE234 pKa = 4.68 SKK236 pKa = 11.17 DD237 pKa = 3.13 IFFFNVPRR245 pKa = 11.84 GGMEE249 pKa = 4.03 FFQYY253 pKa = 10.77 RR254 pKa = 11.84 LAEE257 pKa = 4.14 SLKK260 pKa = 10.7 DD261 pKa = 3.29 RR262 pKa = 11.84 LVFSTKK268 pKa = 9.66 YY269 pKa = 10.1 ASGMKK274 pKa = 9.4 ILHH277 pKa = 6.14 KK278 pKa = 10.34 VPHH281 pKa = 5.78 VVVFCNEE288 pKa = 3.89 APDD291 pKa = 3.99 MTKK294 pKa = 9.45 LTADD298 pKa = 4.67 RR299 pKa = 11.84 IIIHH303 pKa = 6.78 DD304 pKa = 4.16 LL305 pKa = 3.5
Molecular weight: 35.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.609
IPC2_protein 6.576
IPC_protein 6.839
Toseland 6.927
ProMoST 7.19
Dawson 7.293
Bjellqvist 7.102
Wikipedia 7.278
Rodwell 7.278
Grimsley 7.059
Solomon 7.366
Lehninger 7.38
Nozaki 7.424
DTASelect 7.556
Thurlkill 7.585
EMBOSS 7.658
Sillero 7.658
Patrickios 4.418
IPC_peptide 7.38
IPC2_peptide 7.176
IPC2.peptide.svr19 7.088
Protein with the highest isoelectric point:
>tr|A0A0K1RLN2|A0A0K1RLN2_9CIRC Putative replication initiation protein OS=Farfantepenaeus duorarum pink shrimp associated circular virus OX=1692248 PE=4 SV=1
MM1 pKa = 7.24 SAIVPFARR9 pKa = 11.84 YY10 pKa = 8.71 AFPGARR16 pKa = 11.84 RR17 pKa = 11.84 AAAKK21 pKa = 10.53 ALIRR25 pKa = 11.84 TGKK28 pKa = 7.7 ITPRR32 pKa = 11.84 QYY34 pKa = 9.6 MAAYY38 pKa = 9.24 KK39 pKa = 9.98 IGRR42 pKa = 11.84 AAYY45 pKa = 8.39 RR46 pKa = 11.84 WRR48 pKa = 11.84 GIFKK52 pKa = 10.45 SKK54 pKa = 10.19 GYY56 pKa = 9.77 QRR58 pKa = 11.84 SKK60 pKa = 10.6 RR61 pKa = 11.84 RR62 pKa = 11.84 FVRR65 pKa = 11.84 NVRR68 pKa = 11.84 NVVRR72 pKa = 11.84 NVRR75 pKa = 11.84 SAQLKK80 pKa = 5.7 TTHH83 pKa = 7.08 LDD85 pKa = 3.49 FASDD89 pKa = 3.46 EE90 pKa = 4.21 QFTNKK95 pKa = 9.71 LNHH98 pKa = 6.0 LHH100 pKa = 6.62 HH101 pKa = 5.9 VQPYY105 pKa = 8.17 KK106 pKa = 10.63 LVYY109 pKa = 10.57 LGDD112 pKa = 4.22 GDD114 pKa = 4.47 NKK116 pKa = 10.29 RR117 pKa = 11.84 QSSTITCSGLRR128 pKa = 11.84 VQFCFANTQPNGGQPQSHH146 pKa = 6.84 LRR148 pKa = 11.84 TDD150 pKa = 3.35 YY151 pKa = 10.84 DD152 pKa = 2.71 IRR154 pKa = 11.84 MVVLQEE160 pKa = 4.29 KK161 pKa = 8.4 PQPSYY166 pKa = 11.32 VQDD169 pKa = 3.62 DD170 pKa = 3.84 VKK172 pKa = 11.22 KK173 pKa = 10.45 QFFSGRR179 pKa = 11.84 GMAIDD184 pKa = 4.18 YY185 pKa = 7.47 GTIADD190 pKa = 5.2 DD191 pKa = 3.63 FTEE194 pKa = 5.22 AYY196 pKa = 7.62 PKK198 pKa = 10.5 RR199 pKa = 11.84 GIDD202 pKa = 3.72 FQNLSGPMCQKK213 pKa = 8.65 PTYY216 pKa = 9.05 PINKK220 pKa = 8.97 RR221 pKa = 11.84 RR222 pKa = 11.84 WQVLGNKK229 pKa = 10.13 LIFLRR234 pKa = 11.84 RR235 pKa = 11.84 RR236 pKa = 11.84 WDD238 pKa = 3.11 GDD240 pKa = 3.25 YY241 pKa = 11.32 DD242 pKa = 3.82 RR243 pKa = 11.84 QAAGAWWVPLKK254 pKa = 10.73 GKK256 pKa = 10.26 KK257 pKa = 8.31 LTYY260 pKa = 9.57 TVTDD264 pKa = 3.25 TGVEE268 pKa = 4.11 YY269 pKa = 7.42 TTPKK273 pKa = 9.95 IYY275 pKa = 10.37 FMYY278 pKa = 10.57 YY279 pKa = 9.98 IIPHH283 pKa = 6.69 KK284 pKa = 10.66 DD285 pKa = 2.46 ITYY288 pKa = 10.01 NLTHH292 pKa = 6.66 ATTPVKK298 pKa = 10.8 VDD300 pKa = 3.22 IKK302 pKa = 9.68 TIAYY306 pKa = 9.31 FKK308 pKa = 9.09 EE309 pKa = 4.51 TNN311 pKa = 3.14
Molecular weight: 36.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.26
IPC2_protein 9.706
IPC_protein 9.984
Toseland 10.189
ProMoST 9.911
Dawson 10.394
Bjellqvist 10.087
Wikipedia 10.584
Rodwell 10.774
Grimsley 10.482
Solomon 10.423
Lehninger 10.379
Nozaki 10.175
DTASelect 10.087
Thurlkill 10.248
EMBOSS 10.599
Sillero 10.306
Patrickios 10.306
IPC_peptide 10.423
IPC2_peptide 8.858
IPC2.peptide.svr19 8.664
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
616
305
311
308.0
35.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.63 ± 0.169
0.974 ± 0.007
6.169 ± 0.275
3.896 ± 1.65
5.032 ± 0.151
6.331 ± 0.072
2.435 ± 0.133
5.844 ± 0.041
5.844 ± 1.583
7.143 ± 1.209
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.435 ± 0.365
4.383 ± 0.146
5.195 ± 0.036
5.682 ± 0.076
8.929 ± 0.178
3.247 ± 0.441
6.169 ± 1.117
6.006 ± 0.074
1.461 ± 0.106
5.195 ± 0.891
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here