Gordonia phage UmaThurman

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fairfaxidumvirus; unclassified Fairfaxidumvirus

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A142KCU2|A0A142KCU2_9CAUD Minor tail protein OS=Gordonia phage UmaThurman OX=1821563 GN=30 PE=4 SV=1
MM1 pKa = 7.46ANDD4 pKa = 4.61TIFEE8 pKa = 4.28LPEE11 pKa = 4.65IPGVTFTASYY21 pKa = 10.84GSGGEE26 pKa = 4.12TGLPSNWIRR35 pKa = 11.84IVGTVEE41 pKa = 3.91NPWYY45 pKa = 10.09EE46 pKa = 3.64PTYY49 pKa = 11.14NYY51 pKa = 11.06GLDD54 pKa = 3.45PNQYY58 pKa = 9.28TEE60 pKa = 4.86ITDD63 pKa = 2.93PWKK66 pKa = 10.66RR67 pKa = 11.84HH68 pKa = 3.79TQFPEE73 pKa = 3.69VCGPMGFEE81 pKa = 4.68GPSIGLPADD90 pKa = 3.89PPPPPVEE97 pKa = 4.39PEE99 pKa = 3.83PTPDD103 pKa = 3.89IIEE106 pKa = 4.47EE107 pKa = 4.28PTDD110 pKa = 3.47GG111 pKa = 4.67

Molecular weight: 12.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A142KCV3|A0A142KCV3_9CAUD Uncharacterized protein OS=Gordonia phage UmaThurman OX=1821563 GN=41 PE=4 SV=1
MM1 pKa = 6.94VRR3 pKa = 11.84RR4 pKa = 11.84GAAGRR9 pKa = 11.84GQVRR13 pKa = 11.84LGRR16 pKa = 11.84SGSVRR21 pKa = 11.84LGRR24 pKa = 11.84SGRR27 pKa = 11.84GMARR31 pKa = 11.84QVWQGAARR39 pKa = 11.84SGTARR44 pKa = 11.84HH45 pKa = 6.22GPAGMARR52 pKa = 11.84HH53 pKa = 5.73GAVGFGMVGTGSAWARR69 pKa = 11.84QAGLGSARR77 pKa = 11.84CGAVGRR83 pKa = 11.84GVARR87 pKa = 11.84QAWLGRR93 pKa = 11.84VRR95 pKa = 11.84RR96 pKa = 11.84GLAWQAWKK104 pKa = 9.69PLSS107 pKa = 3.6

Molecular weight: 11.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

83

0

83

16237

53

1847

195.6

21.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.48 ± 0.487

0.881 ± 0.134

7.027 ± 0.274

5.321 ± 0.349

2.833 ± 0.116

8.616 ± 0.324

2.156 ± 0.184

4.422 ± 0.158

3.357 ± 0.269

7.421 ± 0.285

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.254 ± 0.173

2.833 ± 0.144

6.042 ± 0.258

3.4 ± 0.13

7.391 ± 0.411

5.469 ± 0.217

6.781 ± 0.235

7.852 ± 0.28

2.149 ± 0.12

2.316 ± 0.178
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski