Amino acid dipepetide frequency for Methylobacterium frigidaeris

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	23.084AlaAla: 23.084 ± 0.199	1.36AlaCys: 1.36 ± 0.033	6.887AlaAsp: 6.887 ± 0.052	9.187AlaGlu: 9.187 ± 0.079	4.661AlaPhe: 4.661 ± 0.056	13.542AlaGly: 13.542 ± 0.114	2.453AlaHis: 2.453 ± 0.037	5.749AlaIle: 5.749 ± 0.062	3.407AlaLys: 3.407 ± 0.071	16.695AlaLeu: 16.695 ± 0.14	3.519AlaMet: 3.519 ± 0.047	2.318AlaAsn: 2.318 ± 0.041	7.504AlaPro: 7.504 ± 0.079	4.03AlaGln: 4.03 ± 0.046	11.981AlaArg: 11.981 ± 0.111	6.45AlaSer: 6.45 ± 0.069	6.669AlaThr: 6.669 ± 0.069	10.918AlaVal: 10.918 ± 0.089	1.869AlaTrp: 1.869 ± 0.034	2.787AlaTyr: 2.787 ± 0.041	0.003AlaXaa: 0.003 ± 0.001
Cys	1.027CysAla: 1.027 ± 0.022	0.097CysCys: 0.097 ± 0.007	0.481CysAsp: 0.481 ± 0.017	0.398CysGlu: 0.398 ± 0.016	0.25CysPhe: 0.25 ± 0.013	0.936CysGly: 0.936 ± 0.023	0.21CysHis: 0.21 ± 0.012	0.305CysIle: 0.305 ± 0.012	0.125CysLys: 0.125 ± 0.01	0.957CysLeu: 0.957 ± 0.024	0.111CysMet: 0.111 ± 0.008	0.163CysAsn: 0.163 ± 0.011	0.491CysPro: 0.491 ± 0.019	0.173CysGln: 0.173 ± 0.009	0.755CysArg: 0.755 ± 0.023	0.361CysSer: 0.361 ± 0.015	0.442CysThr: 0.442 ± 0.019	0.565CysVal: 0.565 ± 0.018	0.106CysTrp: 0.106 ± 0.008	0.153CysTyr: 0.153 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	7.357AspAla: 7.357 ± 0.066	0.378AspCys: 0.378 ± 0.015	2.747AspAsp: 2.747 ± 0.045	3.298AspGlu: 3.298 ± 0.053	1.83AspPhe: 1.83 ± 0.033	5.544AspGly: 5.544 ± 0.07	1.165AspHis: 1.165 ± 0.027	2.073AspIle: 2.073 ± 0.039	1.129AspLys: 1.129 ± 0.034	6.622AspLeu: 6.622 ± 0.074	0.833AspMet: 0.833 ± 0.023	0.82AspAsn: 0.82 ± 0.023	4.146AspPro: 4.146 ± 0.05	1.405AspGln: 1.405 ± 0.029	4.802AspArg: 4.802 ± 0.057	1.674AspSer: 1.674 ± 0.033	2.328AspThr: 2.328 ± 0.04	3.927AspVal: 3.927 ± 0.05	0.83AspTrp: 0.83 ± 0.023	1.207AspTyr: 1.207 ± 0.029	0.001AspXaa: 0.001 ± 0.001
Glu	10.036GluAla: 10.036 ± 0.09	0.297GluCys: 0.297 ± 0.014	2.451GluAsp: 2.451 ± 0.04	2.982GluGlu: 2.982 ± 0.053	1.345GluPhe: 1.345 ± 0.028	4.703GluGly: 4.703 ± 0.063	1.087GluHis: 1.087 ± 0.024	3.005GluIle: 3.005 ± 0.051	1.366GluLys: 1.366 ± 0.033	4.144GluLeu: 4.144 ± 0.056	1.283GluMet: 1.283 ± 0.026	1.042GluAsn: 1.042 ± 0.022	3.206GluPro: 3.206 ± 0.054	1.539GluGln: 1.539 ± 0.034	5.696GluArg: 5.696 ± 0.068	2.202GluSer: 2.202 ± 0.035	3.562GluThr: 3.562 ± 0.054	3.971GluVal: 3.971 ± 0.051	0.577GluTrp: 0.577 ± 0.019	0.745GluTyr: 0.745 ± 0.021	0.001GluXaa: 0.001 ± 0.001
Phe	4.483PheAla: 4.483 ± 0.058	0.36PheCys: 0.36 ± 0.015	2.118PheAsp: 2.118 ± 0.04	1.791PheGlu: 1.791 ± 0.035	1.106PhePhe: 1.106 ± 0.031	3.487PheGly: 3.487 ± 0.058	0.646PheHis: 0.646 ± 0.019	1.072PheIle: 1.072 ± 0.025	0.671PheLys: 0.671 ± 0.021	3.257PheLeu: 3.257 ± 0.05	0.57PheMet: 0.57 ± 0.02	0.759PheAsn: 0.759 ± 0.022	1.584PhePro: 1.584 ± 0.03	0.861PheGln: 0.861 ± 0.024	2.425PheArg: 2.425 ± 0.035	1.875PheSer: 1.875 ± 0.03	1.888PheThr: 1.888 ± 0.035	2.681PheVal: 2.681 ± 0.045	0.438PheTrp: 0.438 ± 0.016	0.758PheTyr: 0.758 ± 0.021	0.0PheXaa: 0.0 ± 0.0
Gly	11.252GlyAla: 11.252 ± 0.106	0.841GlyCys: 0.841 ± 0.022	4.262GlyAsp: 4.262 ± 0.057	4.922GlyGlu: 4.922 ± 0.057	3.553GlyPhe: 3.553 ± 0.049	8.086GlyGly: 8.086 ± 0.095	2.043GlyHis: 2.043 ± 0.043	4.329GlyIle: 4.329 ± 0.051	2.306GlyLys: 2.306 ± 0.043	11.267GlyLeu: 11.267 ± 0.103	2.063GlyMet: 2.063 ± 0.032	1.663GlyAsn: 1.663 ± 0.037	4.996GlyPro: 4.996 ± 0.064	2.939GlyGln: 2.939 ± 0.044	8.322GlyArg: 8.322 ± 0.08	4.482GlySer: 4.482 ± 0.055	5.174GlyThr: 5.174 ± 0.055	6.232GlyVal: 6.232 ± 0.063	1.408GlyTrp: 1.408 ± 0.03	2.249GlyTyr: 2.249 ± 0.043	0.002GlyXaa: 0.002 ± 0.001
His	2.614HisAla: 2.614 ± 0.043	0.2HisCys: 0.2 ± 0.011	1.278HisAsp: 1.278 ± 0.027	0.988HisGlu: 0.988 ± 0.022	0.657HisPhe: 0.657 ± 0.019	2.076HisGly: 2.076 ± 0.032	0.591HisHis: 0.591 ± 0.025	0.637HisIle: 0.637 ± 0.021	0.341HisLys: 0.341 ± 0.014	2.157HisLeu: 2.157 ± 0.042	0.364HisMet: 0.364 ± 0.015	0.355HisAsn: 0.355 ± 0.012	1.525HisPro: 1.525 ± 0.031	0.506HisGln: 0.506 ± 0.017	1.685HisArg: 1.685 ± 0.036	0.703HisSer: 0.703 ± 0.019	0.719HisThr: 0.719 ± 0.025	1.487HisVal: 1.487 ± 0.03	0.315HisTrp: 0.315 ± 0.016	0.495HisTyr: 0.495 ± 0.018	0.0HisXaa: 0.0 ± 0.0
Ile	6.568IleAla: 6.568 ± 0.071	0.354IleCys: 0.354 ± 0.016	2.836IleAsp: 2.836 ± 0.039	2.876IleGlu: 2.876 ± 0.045	1.139IlePhe: 1.139 ± 0.028	4.547IleGly: 4.547 ± 0.059	0.767IleHis: 0.767 ± 0.022	1.301IleIle: 1.301 ± 0.036	0.905IleLys: 0.905 ± 0.028	4.069IleLeu: 4.069 ± 0.059	0.61IleMet: 0.61 ± 0.021	0.933IleAsn: 0.933 ± 0.023	2.087IlePro: 2.087 ± 0.032	1.042IleGln: 1.042 ± 0.026	3.142IleArg: 3.142 ± 0.044	1.718IleSer: 1.718 ± 0.037	2.209IleThr: 2.209 ± 0.037	3.684IleVal: 3.684 ± 0.052	0.442IleTrp: 0.442 ± 0.018	0.811IleTyr: 0.811 ± 0.02	0.001IleXaa: 0.001 ± 0.001
Lys	3.67LysAla: 3.67 ± 0.066	0.095LysCys: 0.095 ± 0.007	1.224LysAsp: 1.224 ± 0.033	1.019LysGlu: 1.019 ± 0.027	0.547LysPhe: 0.547 ± 0.02	2.161LysGly: 2.161 ± 0.042	0.374LysHis: 0.374 ± 0.018	1.019LysIle: 1.019 ± 0.028	0.661LysLys: 0.661 ± 0.024	2.109LysLeu: 2.109 ± 0.039	0.427LysMet: 0.427 ± 0.018	0.529LysAsn: 0.529 ± 0.017	1.769LysPro: 1.769 ± 0.039	0.63LysGln: 0.63 ± 0.019	1.831LysArg: 1.831 ± 0.041	1.127LysSer: 1.127 ± 0.029	1.347LysThr: 1.347 ± 0.031	1.913LysVal: 1.913 ± 0.043	0.207LysTrp: 0.207 ± 0.012	0.382LysTyr: 0.382 ± 0.016	0.001LysXaa: 0.001 ± 0.001
Leu	17.363LeuAla: 17.363 ± 0.158	0.939LeuCys: 0.939 ± 0.022	6.673LeuAsp: 6.673 ± 0.064	4.66LeuGlu: 4.66 ± 0.057	3.339LeuPhe: 3.339 ± 0.053	9.424LeuGly: 9.424 ± 0.093	1.968LeuHis: 1.968 ± 0.039	4.418LeuIle: 4.418 ± 0.054	2.771LeuLys: 2.771 ± 0.043	9.733LeuLeu: 9.733 ± 0.106	2.21LeuMet: 2.21 ± 0.041	2.073LeuAsn: 2.073 ± 0.037	6.437LeuPro: 6.437 ± 0.074	2.438LeuGln: 2.438 ± 0.037	8.251LeuArg: 8.251 ± 0.071	6.181LeuSer: 6.181 ± 0.066	6.052LeuThr: 6.052 ± 0.056	8.695LeuVal: 8.695 ± 0.08	1.172LeuTrp: 1.172 ± 0.025	1.913LeuTyr: 1.913 ± 0.032	0.002LeuXaa: 0.002 ± 0.001
Met	2.815MetAla: 2.815 ± 0.044	0.111MetCys: 0.111 ± 0.009	0.796MetAsp: 0.796 ± 0.022	0.818MetGlu: 0.818 ± 0.023	0.479MetPhe: 0.479 ± 0.018	1.516MetGly: 1.516 ± 0.03	0.374MetHis: 0.374 ± 0.015	1.019MetIle: 1.019 ± 0.027	0.583MetLys: 0.583 ± 0.018	2.151MetLeu: 2.151 ± 0.038	0.507MetMet: 0.507 ± 0.021	0.567MetAsn: 0.567 ± 0.019	1.543MetPro: 1.543 ± 0.026	0.708MetGln: 0.708 ± 0.017	1.933MetArg: 1.933 ± 0.032	1.364MetSer: 1.364 ± 0.029	1.617MetThr: 1.617 ± 0.032	1.438MetVal: 1.438 ± 0.03	0.148MetTrp: 0.148 ± 0.009	0.216MetTyr: 0.216 ± 0.012	0.001MetXaa: 0.001 ± 0.001
Asn	2.641AsnAla: 2.641 ± 0.041	0.173AsnCys: 0.173 ± 0.01	0.987AsnAsp: 0.987 ± 0.029	0.826AsnGlu: 0.826 ± 0.02	0.653AsnPhe: 0.653 ± 0.021	1.895AsnGly: 1.895 ± 0.036	0.387AsnHis: 0.387 ± 0.015	0.755AsnIle: 0.755 ± 0.023	0.43AsnLys: 0.43 ± 0.02	2.198AsnLeu: 2.198 ± 0.034	0.358AsnMet: 0.358 ± 0.015	0.456AsnAsn: 0.456 ± 0.017	1.613AsnPro: 1.613 ± 0.03	0.579AsnGln: 0.579 ± 0.02	1.561AsnArg: 1.561 ± 0.031	0.773AsnSer: 0.773 ± 0.026	0.937AsnThr: 0.937 ± 0.026	1.501AsnVal: 1.501 ± 0.032	0.265AsnTrp: 0.265 ± 0.013	0.472AsnTyr: 0.472 ± 0.018	0.001AsnXaa: 0.001 ± 0.001
Pro	8.629ProAla: 8.629 ± 0.095	0.356ProCys: 0.356 ± 0.016	4.291ProAsp: 4.291 ± 0.059	4.47ProGlu: 4.47 ± 0.054	2.029ProPhe: 2.029 ± 0.036	6.013ProGly: 6.013 ± 0.066	1.171ProHis: 1.171 ± 0.025	2.037ProIle: 2.037 ± 0.037	1.379ProLys: 1.379 ± 0.037	5.429ProLeu: 5.429 ± 0.061	1.161ProMet: 1.161 ± 0.027	1.17ProAsn: 1.17 ± 0.029	3.417ProPro: 3.417 ± 0.055	1.535ProGln: 1.535 ± 0.031	4.075ProArg: 4.075 ± 0.059	2.864ProSer: 2.864 ± 0.043	2.671ProThr: 2.671 ± 0.041	5.222ProVal: 5.222 ± 0.057	0.78ProTrp: 0.78 ± 0.022	1.176ProTyr: 1.176 ± 0.032	0.001ProXaa: 0.001 ± 0.001
Gln	4.487GlnAla: 4.487 ± 0.06	0.15GlnCys: 0.15 ± 0.009	1.491GlnAsp: 1.491 ± 0.032	1.337GlnGlu: 1.337 ± 0.03	0.807GlnPhe: 0.807 ± 0.023	2.568GlnGly: 2.568 ± 0.04	0.494GlnHis: 0.494 ± 0.017	1.432GlnIle: 1.432 ± 0.032	0.73GlnLys: 0.73 ± 0.023	2.206GlnLeu: 2.206 ± 0.035	0.61GlnMet: 0.61 ± 0.019	0.642GlnAsn: 0.642 ± 0.02	1.577GlnPro: 1.577 ± 0.031	0.873GlnGln: 0.873 ± 0.028	2.225GlnArg: 2.225 ± 0.039	1.341GlnSer: 1.341 ± 0.03	1.381GlnThr: 1.381 ± 0.029	2.341GlnVal: 2.341 ± 0.042	0.288GlnTrp: 0.288 ± 0.014	0.482GlnTyr: 0.482 ± 0.018	0.001GlnXaa: 0.001 ± 0.001
Arg	10.834ArgAla: 10.834 ± 0.098	0.589ArgCys: 0.589 ± 0.019	4.923ArgAsp: 4.923 ± 0.056	4.743ArgGlu: 4.743 ± 0.062	3.053ArgPhe: 3.053 ± 0.043	5.962ArgGly: 5.962 ± 0.074	2.012ArgHis: 2.012 ± 0.037	4.068ArgIle: 4.068 ± 0.05	1.692ArgLys: 1.692 ± 0.039	9.882ArgLeu: 9.882 ± 0.094	1.835ArgMet: 1.835 ± 0.037	1.588ArgAsn: 1.588 ± 0.031	5.033ArgPro: 5.033 ± 0.073	2.604ArgGln: 2.604 ± 0.043	7.876ArgArg: 7.876 ± 0.097	3.79ArgSer: 3.79 ± 0.053	4.133ArgThr: 4.133 ± 0.051	5.822ArgVal: 5.822 ± 0.059	1.04ArgTrp: 1.04 ± 0.022	1.704ArgTyr: 1.704 ± 0.033	0.002ArgXaa: 0.002 ± 0.001
Ser	5.935SerAla: 5.935 ± 0.069	0.382SerCys: 0.382 ± 0.014	2.426SerAsp: 2.426 ± 0.041	2.297SerGlu: 2.297 ± 0.038	1.817SerPhe: 1.817 ± 0.036	5.248SerGly: 5.248 ± 0.061	0.916SerHis: 0.916 ± 0.023	1.821SerIle: 1.821 ± 0.032	0.978SerLys: 0.978 ± 0.025	5.649SerLeu: 5.649 ± 0.064	0.853SerMet: 0.853 ± 0.022	0.913SerAsn: 0.913 ± 0.028	2.959SerPro: 2.959 ± 0.049	1.267SerGln: 1.267 ± 0.027	3.664SerArg: 3.664 ± 0.052	2.241SerSer: 2.241 ± 0.049	2.305SerThr: 2.305 ± 0.039	3.569SerVal: 3.569 ± 0.05	0.613SerTrp: 0.613 ± 0.019	1.092SerTyr: 1.092 ± 0.026	0.001SerXaa: 0.001 ± 0.001
Thr	6.752ThrAla: 6.752 ± 0.069	0.446ThrCys: 0.446 ± 0.017	2.646ThrAsp: 2.646 ± 0.042	2.502ThrGlu: 2.502 ± 0.041	1.823ThrPhe: 1.823 ± 0.035	5.57ThrGly: 5.57 ± 0.056	0.991ThrHis: 0.991 ± 0.024	2.449ThrIle: 2.449 ± 0.036	1.102ThrLys: 1.102 ± 0.032	6.199ThrLeu: 6.199 ± 0.066	1.028ThrMet: 1.028 ± 0.025	1.057ThrAsn: 1.057 ± 0.028	3.45ThrPro: 3.45 ± 0.047	1.289ThrGln: 1.289 ± 0.031	3.794ThrArg: 3.794 ± 0.047	2.325ThrSer: 2.325 ± 0.037	2.736ThrThr: 2.736 ± 0.043	4.811ThrVal: 4.811 ± 0.056	0.715ThrTrp: 0.715 ± 0.024	1.165ThrTyr: 1.165 ± 0.027	0.002ThrXaa: 0.002 ± 0.001
Val	11.538ValAla: 11.538 ± 0.087	0.616ValCys: 0.616 ± 0.021	3.685ValAsp: 3.685 ± 0.049	4.617ValGlu: 4.617 ± 0.057	2.523ValPhe: 2.523 ± 0.043	6.009ValGly: 6.009 ± 0.068	1.345ValHis: 1.345 ± 0.03	3.386ValIle: 3.386 ± 0.05	1.751ValLys: 1.751 ± 0.041	8.288ValLeu: 8.288 ± 0.082	1.758ValMet: 1.758 ± 0.034	1.618ValAsn: 1.618 ± 0.03	4.71ValPro: 4.71 ± 0.062	2.082ValGln: 2.082 ± 0.039	5.929ValArg: 5.929 ± 0.058	3.913ValSer: 3.913 ± 0.047	4.934ValThr: 4.934 ± 0.056	6.811ValVal: 6.811 ± 0.077	0.836ValTrp: 0.836 ± 0.021	1.381ValTyr: 1.381 ± 0.029	0.001ValXaa: 0.001 ± 0.001
Trp	1.215TrpAla: 1.215 ± 0.027	0.15TrpCys: 0.15 ± 0.01	0.625TrpAsp: 0.625 ± 0.022	0.461TrpGlu: 0.461 ± 0.016	0.511TrpPhe: 0.511 ± 0.019	0.916TrpGly: 0.916 ± 0.024	0.311TrpHis: 0.311 ± 0.014	0.576TrpIle: 0.576 ± 0.02	0.293TrpLys: 0.293 ± 0.015	1.564TrpLeu: 1.564 ± 0.033	0.297TrpMet: 0.297 ± 0.013	0.351TrpAsn: 0.351 ± 0.014	0.745TrpPro: 0.745 ± 0.022	0.452TrpGln: 0.452 ± 0.018	1.362TrpArg: 1.362 ± 0.031	0.768TrpSer: 0.768 ± 0.022	0.796TrpThr: 0.796 ± 0.026	0.721TrpVal: 0.721 ± 0.023	0.222TrpTrp: 0.222 ± 0.012	0.252TrpTyr: 0.252 ± 0.013	0.001TrpXaa: 0.001 ± 0.001
Tyr	2.769TyrAla: 2.769 ± 0.041	0.209TyrCys: 0.209 ± 0.011	1.288TyrAsp: 1.288 ± 0.027	1.044TyrGlu: 1.044 ± 0.023	0.708TyrPhe: 0.708 ± 0.023	2.185TyrGly: 2.185 ± 0.04	0.386TyrHis: 0.386 ± 0.014	0.59TyrIle: 0.59 ± 0.02	0.42TyrLys: 0.42 ± 0.018	2.138TyrLeu: 2.138 ± 0.037	0.305TyrMet: 0.305 ± 0.014	0.445TyrAsn: 0.445 ± 0.018	1.081TyrPro: 1.081 ± 0.025	0.511TyrGln: 0.511 ± 0.019	1.877TyrArg: 1.877 ± 0.036	0.792TyrSer: 0.792 ± 0.026	0.952TyrThr: 0.952 ± 0.026	1.423TyrVal: 1.423 ± 0.032	0.318TyrTrp: 0.318 ± 0.014	0.494TyrTyr: 0.494 ± 0.017	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.002XaaAla: 0.002 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.001XaaGlu: 0.001 ± 0.001	0.001XaaPhe: 0.001 ± 0.001	0.001XaaGly: 0.001 ± 0.001	0.001XaaHis: 0.001 ± 0.001	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.004XaaPro: 0.004 ± 0.002	0.0XaaGln: 0.0 ± 0.0	0.002XaaArg: 0.002 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.002XaaThr: 0.002 ± 0.001	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.003XaaXaa: 0.003 ± 0.002

Statistics based on 6432 proteins (1677552 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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