Bathycoccus sp. RCC1105 virus BpV2
Average proteome isoelectric point is 6.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 210 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E5ER03|E5ER03_9PHYC Uncharacterized protein OS=Bathycoccus sp. RCC1105 virus BpV2 OX=880160 GN=BpV2_072c PE=4 SV=1
MM1 pKa = 6.96 EE2 pKa = 5.85 AEE4 pKa = 4.08 VGTPINYY11 pKa = 10.21 DD12 pKa = 3.08 PDD14 pKa = 3.42 EE15 pKa = 5.32 FINKK19 pKa = 8.92 EE20 pKa = 3.61 QDD22 pKa = 3.17 QKK24 pKa = 11.56 LDD26 pKa = 3.56 EE27 pKa = 5.03 PEE29 pKa = 4.58 PEE31 pKa = 4.31 NNEE34 pKa = 3.91 HH35 pKa = 6.84 YY36 pKa = 10.63 YY37 pKa = 10.51 FPPPQQYY44 pKa = 9.64 YY45 pKa = 9.96 EE46 pKa = 5.05 PYY48 pKa = 9.07 PQQPTQKK55 pKa = 10.42 EE56 pKa = 4.31 DD57 pKa = 2.74 IFTNLDD63 pKa = 2.89 KK64 pKa = 10.44 TAYY67 pKa = 9.61 IIIFVSFILGFFMGKK82 pKa = 7.69 TMQPVILRR90 pKa = 11.84 PGG92 pKa = 3.13
Molecular weight: 10.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.923
IPC2_protein 4.228
IPC_protein 4.101
Toseland 3.935
ProMoST 4.215
Dawson 4.05
Bjellqvist 4.202
Wikipedia 3.935
Rodwell 3.935
Grimsley 3.846
Solomon 4.037
Lehninger 3.986
Nozaki 4.177
DTASelect 4.291
Thurlkill 3.961
EMBOSS 3.948
Sillero 4.202
Patrickios 1.939
IPC_peptide 4.05
IPC2_peptide 4.19
IPC2.peptide.svr19 4.115
Protein with the highest isoelectric point:
>tr|E5ER16|E5ER16_9PHYC Uncharacterized protein OS=Bathycoccus sp. RCC1105 virus BpV2 OX=880160 GN=BpV2_085c PE=4 SV=1
MM1 pKa = 6.78 STAAEE6 pKa = 4.31 TVTLVARR13 pKa = 11.84 EE14 pKa = 4.53 LEE16 pKa = 4.44 SQSLNAVVAGFSFAAALSWMDD37 pKa = 3.41 LVRR40 pKa = 11.84 WLVNQVVKK48 pKa = 10.87 VNKK51 pKa = 9.79 NGGMNYY57 pKa = 9.07 TLTALFTTLLSILVYY72 pKa = 10.63 VGISRR77 pKa = 11.84 VSTRR81 pKa = 11.84 VQKK84 pKa = 8.72 PTQPIFAVTRR94 pKa = 3.88
Molecular weight: 10.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.254
IPC2_protein 9.575
IPC_protein 10.058
Toseland 10.306
ProMoST 10.365
Dawson 10.467
Bjellqvist 10.145
Wikipedia 10.657
Rodwell 10.789
Grimsley 10.54
Solomon 10.54
Lehninger 10.511
Nozaki 10.262
DTASelect 10.16
Thurlkill 10.335
EMBOSS 10.701
Sillero 10.379
Patrickios 10.657
IPC_peptide 10.54
IPC2_peptide 8.829
IPC2.peptide.svr19 8.73
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
210
0
210
57839
65
3675
275.4
31.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.841 ± 0.208
1.452 ± 0.131
6.074 ± 0.175
6.203 ± 0.329
4.226 ± 0.104
6.857 ± 0.505
1.864 ± 0.097
7.016 ± 0.209
7.977 ± 0.454
7.478 ± 0.253
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.593 ± 0.157
6.385 ± 0.307
3.838 ± 0.139
3.041 ± 0.116
4.179 ± 0.227
7.039 ± 0.34
7.331 ± 0.398
6.036 ± 0.145
1.06 ± 0.081
4.511 ± 0.163
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here