Streptococcus satellite phage Javan365

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZLA1|A0A4D5ZLA1_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan365 OX=2558663 GN=JavanS365_0004 PE=4 SV=1
MM1 pKa = 7.39LVTYY5 pKa = 8.27PALFYY10 pKa = 11.35YY11 pKa = 10.68DD12 pKa = 4.04DD13 pKa = 4.01TDD15 pKa = 3.86GANAPYY21 pKa = 10.03FVTFPDD27 pKa = 4.61FEE29 pKa = 5.3HH30 pKa = 7.07SATQGEE36 pKa = 4.5DD37 pKa = 3.09MADD40 pKa = 3.52AMAMASDD47 pKa = 3.34WLGINLADD55 pKa = 3.95YY56 pKa = 10.56IEE58 pKa = 4.57NGRR61 pKa = 11.84EE62 pKa = 3.92IPTPTPINALSLASNNPFQNDD83 pKa = 2.86EE84 pKa = 5.13DD85 pKa = 4.18IDD87 pKa = 4.23LVYY90 pKa = 10.97NPSKK94 pKa = 11.02SFISMVMVDD103 pKa = 3.25VAEE106 pKa = 4.14YY107 pKa = 10.49LGSQEE112 pKa = 4.15PVKK115 pKa = 10.18KK116 pKa = 9.12TLTIPRR122 pKa = 11.84WADD125 pKa = 3.11TLGRR129 pKa = 11.84DD130 pKa = 3.39LGLNFSQTLTDD141 pKa = 5.45AIADD145 pKa = 4.03KK146 pKa = 10.56KK147 pKa = 10.31IHH149 pKa = 5.92AA150 pKa = 5.0

Molecular weight: 16.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZLM8|A0A4D5ZLM8_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan365 OX=2558663 GN=JavanS365_0002 PE=4 SV=1
MM1 pKa = 7.62KK2 pKa = 10.17DD3 pKa = 3.15KK4 pKa = 11.04NISHH8 pKa = 7.45GIAEE12 pKa = 4.31LDD14 pKa = 3.6GRR16 pKa = 11.84KK17 pKa = 9.93LEE19 pKa = 4.22IDD21 pKa = 4.03LDD23 pKa = 3.6NLYY26 pKa = 10.32IIFGSNHH33 pKa = 6.29FDD35 pKa = 3.38LASIPGTKK43 pKa = 9.9GGNRR47 pKa = 11.84YY48 pKa = 9.37FFVCPICGNRR58 pKa = 11.84CRR60 pKa = 11.84KK61 pKa = 9.4LYY63 pKa = 10.46KK64 pKa = 10.14RR65 pKa = 11.84LLLYY69 pKa = 11.0GCGSCQKK76 pKa = 9.71IHH78 pKa = 7.2KK79 pKa = 8.43STLNRR84 pKa = 11.84SKK86 pKa = 9.89TDD88 pKa = 3.16CQYY91 pKa = 9.13YY92 pKa = 8.45WEE94 pKa = 4.55RR95 pKa = 11.84ALRR98 pKa = 11.84EE99 pKa = 4.03ARR101 pKa = 11.84KK102 pKa = 9.86VEE104 pKa = 4.41PGWSPKK110 pKa = 9.98RR111 pKa = 11.84GGYY114 pKa = 8.86MFDD117 pKa = 4.01SFPKK121 pKa = 9.36RR122 pKa = 11.84PKK124 pKa = 8.96YY125 pKa = 9.61MKK127 pKa = 9.36RR128 pKa = 11.84NKK130 pKa = 9.95YY131 pKa = 8.29YY132 pKa = 10.26KK133 pKa = 10.05HH134 pKa = 4.81YY135 pKa = 10.61QKK137 pKa = 10.68FVNYY141 pKa = 7.53TKK143 pKa = 10.68KK144 pKa = 10.82GDD146 pKa = 4.04SFWLNGLSNLKK157 pKa = 10.28

Molecular weight: 18.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18

0

18

3067

42

499

170.4

19.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.673 ± 0.407

0.88 ± 0.205

5.934 ± 0.621

8.151 ± 0.823

4.369 ± 0.315

5.543 ± 0.481

1.891 ± 0.276

6.554 ± 0.417

9.097 ± 0.495

9.782 ± 0.523

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.706 ± 0.41

5.217 ± 0.362

3.587 ± 0.549

3.424 ± 0.241

4.923 ± 0.422

5.51 ± 0.466

5.934 ± 0.352

5.282 ± 0.473

1.076 ± 0.152

4.467 ± 0.375
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski