Olsenella sp. oral taxon 809 str. F0356
Average proteome isoelectric point is 5.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1898 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G5F643|G5F643_9ACTN PTS EIIA type-2 domain-containing protein OS=Olsenella sp. oral taxon 809 str. F0356 OX=661087 GN=HMPREF1008_01837 PE=4 SV=1
MM1 pKa = 7.54 SISISRR7 pKa = 11.84 RR8 pKa = 11.84 SFLASCGLGLAAEE21 pKa = 4.52 LAGCSAQGGGSAGGTAGSATEE42 pKa = 3.8 AGYY45 pKa = 8.47 TLVEE49 pKa = 4.31 PGKK52 pKa = 9.37 LTMISNFYY60 pKa = 10.41 FPPFVSMNADD70 pKa = 3.12 TGDD73 pKa = 3.62 YY74 pKa = 11.08 EE75 pKa = 5.16 GFDD78 pKa = 3.3 VDD80 pKa = 5.35 LYY82 pKa = 11.42 KK83 pKa = 10.71 AVCEE87 pKa = 4.07 KK88 pKa = 11.0 LGLEE92 pKa = 4.16 PNILPTVQFDD102 pKa = 4.33 TIVPTIKK109 pKa = 10.24 QGGKK113 pKa = 10.2 ADD115 pKa = 3.6 VSLGAVTITDD125 pKa = 3.44 EE126 pKa = 4.19 RR127 pKa = 11.84 LQEE130 pKa = 3.8 IDD132 pKa = 3.89 FSNPYY137 pKa = 9.96 LDD139 pKa = 3.92 SNQAIVVKK147 pKa = 10.29 QGSAATTADD156 pKa = 3.54 ALNASGTQIAVQSGTTGEE174 pKa = 3.75 AWSRR178 pKa = 11.84 EE179 pKa = 4.0 NLPNATIVPLDD190 pKa = 5.35 DD191 pKa = 6.09 IIQCLTGVQTGLYY204 pKa = 8.85 AASVCDD210 pKa = 4.47 LPVASYY216 pKa = 9.8 EE217 pKa = 4.01 ISSAYY222 pKa = 9.95 ADD224 pKa = 4.03 LVIPDD229 pKa = 4.67 GGQIPTGEE237 pKa = 3.97 QYY239 pKa = 11.29 GIVVSKK245 pKa = 10.71 DD246 pKa = 3.27 NPKK249 pKa = 8.87 LTQAINDD256 pKa = 4.15 ALVEE260 pKa = 4.11 LQSDD264 pKa = 4.53 GTQDD268 pKa = 3.7 QIEE271 pKa = 4.39 QKK273 pKa = 10.05 WFGSTII279 pKa = 3.33
Molecular weight: 29.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.754
IPC2_protein 3.91
IPC_protein 3.897
Toseland 3.681
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.808
Rodwell 3.719
Grimsley 3.592
Solomon 3.872
Lehninger 3.834
Nozaki 3.999
DTASelect 4.215
Thurlkill 3.732
EMBOSS 3.821
Sillero 4.012
Patrickios 1.901
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.917
Protein with the highest isoelectric point:
>tr|G5F2C6|G5F2C6_9ACTN Non-specific serine/threonine protein kinase OS=Olsenella sp. oral taxon 809 str. F0356 OX=661087 GN=HMPREF1008_00520 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.34 QPNKK9 pKa = 8.62 RR10 pKa = 11.84 KK11 pKa = 9.56 RR12 pKa = 11.84 AKK14 pKa = 8.76 THH16 pKa = 5.23 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.11 GGRR28 pKa = 11.84 AVLSRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.16 GRR39 pKa = 11.84 KK40 pKa = 8.12 QLTVV44 pKa = 2.97
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.414
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.384
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.106
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.021
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1898
0
1898
624498
30
1736
329.0
35.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.185 ± 0.067
1.48 ± 0.024
6.337 ± 0.048
6.641 ± 0.056
3.366 ± 0.033
9.014 ± 0.042
1.9 ± 0.025
4.108 ± 0.045
3.128 ± 0.045
10.001 ± 0.071
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.509 ± 0.025
2.42 ± 0.033
4.389 ± 0.034
2.968 ± 0.031
7.095 ± 0.064
6.401 ± 0.051
4.835 ± 0.043
8.501 ± 0.049
1.096 ± 0.021
2.627 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here