Bacteroidales bacterium WCE2008

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; unclassified Bacteroidales

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1918 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1T5IEW2|A0A1T5IEW2_9BACT Uncharacterized protein OS=Bacteroidales bacterium WCE2008 OX=1945891 GN=SAMN06298215_0474 PE=4 SV=1
MM1 pKa = 7.48DD2 pKa = 3.31QKK4 pKa = 10.66IYY6 pKa = 10.98HH7 pKa = 6.17NVPEE11 pKa = 5.45GYY13 pKa = 10.4FEE15 pKa = 4.53DD16 pKa = 3.88LKK18 pKa = 10.43TRR20 pKa = 11.84LSAIPQEE27 pKa = 4.4EE28 pKa = 4.42HH29 pKa = 6.08ASGWEE34 pKa = 3.7RR35 pKa = 11.84LRR37 pKa = 11.84PYY39 pKa = 10.11VALAAAFVAIVTVGTAVLRR58 pKa = 11.84LSTSPAAQDD67 pKa = 3.47YY68 pKa = 10.83DD69 pKa = 3.76DD70 pKa = 5.79FGIAEE75 pKa = 5.46IIPSTDD81 pKa = 2.5SYY83 pKa = 11.2IYY85 pKa = 10.05YY86 pKa = 9.99SSEE89 pKa = 3.73VSEE92 pKa = 4.76EE93 pKa = 4.16PVSSEE98 pKa = 4.93DD99 pKa = 5.65DD100 pKa = 3.24ILEE103 pKa = 4.06YY104 pKa = 11.04LLNSNTSVEE113 pKa = 4.2QIAYY117 pKa = 10.11LGNEE121 pKa = 3.93NN122 pKa = 4.03

Molecular weight: 13.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1T5I8B4|A0A1T5I8B4_9BACT Transcriptional regulator LytTR family OS=Bacteroidales bacterium WCE2008 OX=1945891 GN=SAMN06298215_0222 PE=4 SV=1
MM1 pKa = 7.89ADD3 pKa = 3.44NTTSLQRR10 pKa = 11.84HH11 pKa = 5.49NNMAAIHH18 pKa = 6.32SDD20 pKa = 3.11STKK23 pKa = 10.83PEE25 pKa = 3.7MTLRR29 pKa = 11.84RR30 pKa = 11.84ALWGRR35 pKa = 11.84GFRR38 pKa = 11.84YY39 pKa = 7.63RR40 pKa = 11.84TNVRR44 pKa = 11.84SLPGSPDD51 pKa = 2.75IVLPRR56 pKa = 11.84YY57 pKa = 7.67RR58 pKa = 11.84TAIFVNGCFWHH69 pKa = 5.59GHH71 pKa = 5.29RR72 pKa = 11.84GCRR75 pKa = 11.84NYY77 pKa = 9.95TVPKK81 pKa = 9.26TNTEE85 pKa = 3.64FWVAKK90 pKa = 8.51VARR93 pKa = 11.84NQEE96 pKa = 3.66RR97 pKa = 11.84DD98 pKa = 3.51QVVWRR103 pKa = 11.84MLEE106 pKa = 3.85AKK108 pKa = 8.9GWSVVIVWEE117 pKa = 4.36CEE119 pKa = 3.99LKK121 pKa = 10.62KK122 pKa = 10.87ACLDD126 pKa = 3.47ATVDD130 pKa = 3.8RR131 pKa = 11.84VCAEE135 pKa = 3.23IRR137 pKa = 11.84RR138 pKa = 11.84NGEE141 pKa = 3.68RR142 pKa = 11.84YY143 pKa = 9.38RR144 pKa = 11.84EE145 pKa = 3.83FQAARR150 pKa = 11.84RR151 pKa = 11.84KK152 pKa = 9.94ARR154 pKa = 11.84EE155 pKa = 3.73EE156 pKa = 3.55YY157 pKa = 10.18RR158 pKa = 11.84RR159 pKa = 11.84EE160 pKa = 3.48QRR162 pKa = 11.84ARR164 pKa = 11.84KK165 pKa = 8.68EE166 pKa = 4.76RR167 pKa = 11.84EE168 pKa = 3.98AQWRR172 pKa = 11.84ADD174 pKa = 3.2LKK176 pKa = 11.19KK177 pKa = 10.77YY178 pKa = 10.98VNLL181 pKa = 4.07

Molecular weight: 21.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1918

0

1918

741257

31

2408

386.5

43.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.005 ± 0.057

1.194 ± 0.018

6.233 ± 0.035

6.376 ± 0.048

4.497 ± 0.034

7.659 ± 0.055

1.679 ± 0.022

6.647 ± 0.053

6.061 ± 0.048

8.61 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.709 ± 0.024

4.351 ± 0.042

3.897 ± 0.026

2.591 ± 0.025

4.912 ± 0.044

6.765 ± 0.054

5.296 ± 0.042

6.969 ± 0.048

1.244 ± 0.023

4.303 ± 0.048
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski