Pelomonas puraquae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Pelomonas

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5356 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A254NHP6|A0A254NHP6_9BURK Uncharacterized protein OS=Pelomonas puraquae OX=431059 GN=CDO81_09225 PE=4 SV=1
MM1 pKa = 7.59CLICGWIYY9 pKa = 11.28DD10 pKa = 4.41EE11 pKa = 4.58ATGDD15 pKa = 4.1PDD17 pKa = 4.31HH18 pKa = 7.22GIPPGTAWADD28 pKa = 2.71VDD30 pKa = 4.56MNWTCPDD37 pKa = 3.14CGARR41 pKa = 11.84KK42 pKa = 9.56EE43 pKa = 4.07DD44 pKa = 3.8FEE46 pKa = 4.47MVRR49 pKa = 11.84II50 pKa = 4.06

Molecular weight: 5.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A254N9J2|A0A254N9J2_9BURK Uncharacterized protein OS=Pelomonas puraquae OX=431059 GN=CDO81_20360 PE=4 SV=1
MM1 pKa = 7.33KK2 pKa = 9.38RR3 pKa = 11.84TYY5 pKa = 9.73QASKK9 pKa = 7.81TRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.79GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.8GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5356

0

5356

1837375

26

4071

343.0

37.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.781 ± 0.055

0.851 ± 0.01

5.266 ± 0.022

5.081 ± 0.032

3.274 ± 0.02

8.423 ± 0.037

2.168 ± 0.016

3.631 ± 0.026

2.847 ± 0.029

11.471 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.157 ± 0.016

2.375 ± 0.021

5.563 ± 0.031

4.201 ± 0.026

7.45 ± 0.033

5.199 ± 0.029

5.177 ± 0.036

7.438 ± 0.025

1.624 ± 0.016

2.022 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski