Hubei permutotetra-like virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KHW1|A0A1L3KHW1_9VIRU RdRp OS=Hubei permutotetra-like virus 2 OX=1923076 PE=4 SV=1
MM1 pKa = 7.26YY2 pKa = 10.49LSQRR6 pKa = 11.84QFTNHH11 pKa = 4.34TTANHH16 pKa = 6.65LDD18 pKa = 3.68DD19 pKa = 5.11GVWIGLPEE27 pKa = 4.17MMTEE31 pKa = 3.76NDD33 pKa = 3.32VNGAYY38 pKa = 10.11ALSSGPIPINIQNAKK53 pKa = 9.39QNTVIMNSLNTPAHH67 pKa = 5.41GVIKK71 pKa = 10.19RR72 pKa = 11.84VPLILDD78 pKa = 3.96TYY80 pKa = 11.08LVIRR84 pKa = 11.84TNFTLNTGDD93 pKa = 4.11LSSVICSLKK102 pKa = 10.87PNDD105 pKa = 4.25QSKK108 pKa = 9.59PWDD111 pKa = 3.72IPWTGVPIVDD121 pKa = 4.92KK122 pKa = 9.81FVCQSGKK129 pKa = 10.43KK130 pKa = 10.05FYY132 pKa = 10.45TLATNLLLRR141 pKa = 11.84CDD143 pKa = 3.84KK144 pKa = 10.66VRR146 pKa = 11.84PADD149 pKa = 5.98FYY151 pKa = 11.34FEE153 pKa = 4.14WQLEE157 pKa = 4.26FNTPFSSVWNFVMEE171 pKa = 4.18YY172 pKa = 9.91TLDD175 pKa = 4.13LLTAPYY181 pKa = 10.03MNMNIVPSLTYY192 pKa = 9.34TDD194 pKa = 4.23RR195 pKa = 11.84DD196 pKa = 3.89MEE198 pKa = 4.77HH199 pKa = 7.37KK200 pKa = 10.34LQNLQIEE207 pKa = 4.47SDD209 pKa = 3.83DD210 pKa = 3.36TDD212 pKa = 3.62EE213 pKa = 4.64QHH215 pKa = 6.88EE216 pKa = 4.42SDD218 pKa = 4.14YY219 pKa = 11.56NLPQTLYY226 pKa = 9.76PPLPSAPMAEE236 pKa = 3.75AVIPQKK242 pKa = 10.77PKK244 pKa = 8.55TKK246 pKa = 10.27RR247 pKa = 11.84GILTRR252 pKa = 11.84LFKK255 pKa = 10.82RR256 pKa = 11.84KK257 pKa = 9.17IYY259 pKa = 10.57KK260 pKa = 9.57PP261 pKa = 3.49

Molecular weight: 29.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KHV3|A0A1L3KHV3_9VIRU Uncharacterized protein OS=Hubei permutotetra-like virus 2 OX=1923076 PE=4 SV=1
MM1 pKa = 7.86PNNKK5 pKa = 9.18NMNKK9 pKa = 9.94KK10 pKa = 9.11GQKK13 pKa = 9.64KK14 pKa = 8.4PQKK17 pKa = 9.71QKK19 pKa = 11.14QKK21 pKa = 10.71VKK23 pKa = 10.2PQLQSNRR30 pKa = 11.84SANRR34 pKa = 11.84KK35 pKa = 6.98PQAVNLLMGVPVTGRR50 pKa = 11.84EE51 pKa = 3.98VTTTLTAAANKK62 pKa = 9.76TSVSYY67 pKa = 11.35KK68 pKa = 8.56NTFNISTASPLLKK81 pKa = 10.22KK82 pKa = 10.52YY83 pKa = 8.97ATIYY87 pKa = 10.28EE88 pKa = 4.33SYY90 pKa = 10.27KK91 pKa = 10.32IKK93 pKa = 9.65TVSYY97 pKa = 10.48RR98 pKa = 11.84FIPDD102 pKa = 3.16EE103 pKa = 4.6SGLSSGNISIGIDD116 pKa = 3.24YY117 pKa = 10.68GKK119 pKa = 10.81APGDD123 pKa = 3.44LTRR126 pKa = 11.84EE127 pKa = 4.17QISRR131 pKa = 11.84LNPHH135 pKa = 6.05YY136 pKa = 10.5SGPIRR141 pKa = 11.84KK142 pKa = 7.5ITPWITIHH150 pKa = 6.31PRR152 pKa = 11.84FVSTDD157 pKa = 3.25TVRR160 pKa = 11.84YY161 pKa = 10.08SGDD164 pKa = 3.72DD165 pKa = 3.47SLMSAPFTLALLATCEE181 pKa = 4.35SKK183 pKa = 10.69PADD186 pKa = 3.76RR187 pKa = 11.84TLGCVEE193 pKa = 3.72IQYY196 pKa = 9.03TLEE199 pKa = 4.02FQGILPP205 pKa = 4.34

Molecular weight: 22.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1480

205

1014

493.3

55.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.081 ± 0.737

0.811 ± 0.153

5.811 ± 0.732

5.541 ± 1.073

2.838 ± 0.362

5.405 ± 0.718

1.757 ± 0.239

4.392 ± 1.164

8.446 ± 0.984

10.0 ± 0.674

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.108 ± 0.353

3.919 ± 1.581

6.486 ± 0.72

4.797 ± 0.073

4.324 ± 0.328

6.351 ± 0.891

7.703 ± 0.64

6.959 ± 0.917

1.486 ± 0.312

3.784 ± 0.289
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski