Rubricoccus marinus
Average proteome isoelectric point is 5.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3669 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A259TZK3|A0A259TZK3_9BACT N-acetyltransferase domain-containing protein OS=Rubricoccus marinus OX=716817 GN=BSZ36_09470 PE=4 SV=1
MM1 pKa = 7.76 RR2 pKa = 11.84 SLRR5 pKa = 11.84 IPAVAVLAAAFAMAGCDD22 pKa = 3.21 NGPNTRR28 pKa = 11.84 TYY30 pKa = 10.2 TADD33 pKa = 3.49 LSALNGSGASGVATITVDD51 pKa = 3.19 NEE53 pKa = 4.66 SNVVSVSLTASGLDD67 pKa = 3.65 DD68 pKa = 4.78 TIHH71 pKa = 6.06 AQHH74 pKa = 5.8 VHH76 pKa = 5.42 SAGSIISSCPTAADD90 pKa = 3.92 DD91 pKa = 4.74 ANSDD95 pKa = 3.69 GYY97 pKa = 11.43 VDD99 pKa = 4.01 VVEE102 pKa = 4.98 GLPKK106 pKa = 10.21 YY107 pKa = 10.86 GPIILPLDD115 pKa = 3.76 NDD117 pKa = 3.54 ISGADD122 pKa = 3.53 ANVSGFPTGTSIDD135 pKa = 4.02 YY136 pKa = 7.57 TTSAPRR142 pKa = 11.84 ADD144 pKa = 3.56 VADD147 pKa = 4.11 AVDD150 pKa = 3.92 NDD152 pKa = 3.91 GFLDD156 pKa = 3.99 FQDD159 pKa = 3.29 WAIVVHH165 pKa = 6.36 GTTEE169 pKa = 4.21 DD170 pKa = 3.67 LPSTVATLPGSGLSNQVTLPVACGAIRR197 pKa = 11.84 FQQ199 pKa = 4.2
Molecular weight: 20.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.711
IPC2_protein 3.795
IPC_protein 3.821
Toseland 3.579
ProMoST 4.024
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.834
Rodwell 3.643
Grimsley 3.49
Solomon 3.834
Lehninger 3.795
Nozaki 3.961
DTASelect 4.291
Thurlkill 3.656
EMBOSS 3.834
Sillero 3.948
Patrickios 1.926
IPC_peptide 3.821
IPC2_peptide 3.923
IPC2.peptide.svr19 3.854
Protein with the highest isoelectric point:
>tr|A0A259TV65|A0A259TV65_9BACT Uncharacterized protein OS=Rubricoccus marinus OX=716817 GN=BSZ36_17325 PE=4 SV=1
MM1 pKa = 7.33 AAAAALKK8 pKa = 10.13 RR9 pKa = 11.84 RR10 pKa = 11.84 VVARR14 pKa = 11.84 APHH17 pKa = 5.73 PVGVSSVRR25 pKa = 11.84 LVPVRR30 pKa = 11.84 APRR33 pKa = 11.84 PVPSPLRR40 pKa = 11.84 LVLGGRR46 pKa = 11.84 PALDD50 pKa = 2.91 VWAATVPTPALVQRR64 pKa = 11.84 GVFPVWHH71 pKa = 7.24 DD72 pKa = 3.51 VRR74 pKa = 11.84 PGTQRR79 pKa = 11.84 RR80 pKa = 11.84 GVLRR84 pKa = 11.84 WRR86 pKa = 11.84 RR87 pKa = 11.84 GGGGGRR93 pKa = 11.84 EE94 pKa = 3.9 AAGTVGLQPASVRR107 pKa = 11.84 RR108 pKa = 11.84 AA109 pKa = 3.12
Molecular weight: 11.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.358
IPC2_protein 10.774
IPC_protein 12.384
Toseland 12.544
ProMoST 13.042
Dawson 12.544
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.076
Grimsley 12.588
Solomon 13.042
Lehninger 12.939
Nozaki 12.544
DTASelect 12.544
Thurlkill 12.544
EMBOSS 13.042
Sillero 12.544
Patrickios 11.798
IPC_peptide 13.042
IPC2_peptide 12.032
IPC2.peptide.svr19 9.178
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3669
0
3669
1282544
38
4150
349.6
37.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.285 ± 0.06
0.567 ± 0.011
6.28 ± 0.035
6.341 ± 0.037
3.444 ± 0.024
9.097 ± 0.039
1.858 ± 0.022
3.632 ± 0.033
1.881 ± 0.028
10.144 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.797 ± 0.019
2.095 ± 0.026
5.67 ± 0.032
2.863 ± 0.023
7.677 ± 0.048
5.655 ± 0.028
5.847 ± 0.039
8.126 ± 0.034
1.334 ± 0.017
2.408 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here