Streptomyces olivochromogenes

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10549 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A250VIR4|A0A250VIR4_STROL Transcriptional regulator OS=Streptomyces olivochromogenes OX=1963 GN=SO3561_05609 PE=4 SV=1
MM1 pKa = 7.59ANLAPGLPDD10 pKa = 3.63GVDD13 pKa = 3.31VEE15 pKa = 5.44IDD17 pKa = 3.48VDD19 pKa = 3.86WYY21 pKa = 9.6DD22 pKa = 3.71TPAVVDD28 pKa = 4.21CGQNLLRR35 pKa = 11.84IGCPRR40 pKa = 11.84CDD42 pKa = 4.51ASIDD46 pKa = 3.94TQWWSDD52 pKa = 3.36LLEE55 pKa = 4.13AHH57 pKa = 6.75GEE59 pKa = 4.19DD60 pKa = 4.61GFGTLAIEE68 pKa = 4.7VPCCGAATSLDD79 pKa = 3.5ALDD82 pKa = 4.68YY83 pKa = 10.93DD84 pKa = 4.43WPCGFARR91 pKa = 11.84FEE93 pKa = 3.94IAVWNPEE100 pKa = 4.07RR101 pKa = 11.84LWFSDD106 pKa = 3.54EE107 pKa = 4.02EE108 pKa = 4.4LTVLGDD114 pKa = 3.95ALGHH118 pKa = 5.75PVRR121 pKa = 11.84QIRR124 pKa = 11.84AHH126 pKa = 5.41II127 pKa = 3.97

Molecular weight: 13.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A250VJA8|A0A250VJA8_STROL Serine/threonine protein kinase OS=Streptomyces olivochromogenes OX=1963 GN=SO3561_05676 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.51GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10549

0

10549

3319167

29

7994

314.6

33.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.307 ± 0.037

0.794 ± 0.006

5.903 ± 0.019

5.5 ± 0.023

2.745 ± 0.013

9.322 ± 0.023

2.381 ± 0.012

3.249 ± 0.017

2.263 ± 0.022

10.257 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.739 ± 0.009

1.873 ± 0.013

6.086 ± 0.023

2.871 ± 0.014

7.9 ± 0.027

5.376 ± 0.02

6.392 ± 0.022

8.353 ± 0.022

1.562 ± 0.01

2.127 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski