Cotton leaf curl Allahabad virus [India:Karnal:OY81B:2005]
Average proteome isoelectric point is 8.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E7BNJ2|E7BNJ2_9GEMI AC4 protein OS=Cotton leaf curl Allahabad virus [India:Karnal:OY81B:2005] OX=908136 GN=AC4 PE=3 SV=1
MM1 pKa = 7.59 PRR3 pKa = 11.84 NNSFCINAKK12 pKa = 10.45 NIFLTFPKK20 pKa = 10.37 CPIPKK25 pKa = 9.7 EE26 pKa = 3.87 QMLDD30 pKa = 2.9 ILSNISCPSDD40 pKa = 3.09 KK41 pKa = 11.04 LFIRR45 pKa = 11.84 VAQEE49 pKa = 3.46 KK50 pKa = 9.49 HH51 pKa = 5.19 QDD53 pKa = 3.38 GSLHH57 pKa = 5.16 IHH59 pKa = 6.91 ALIQFKK65 pKa = 10.83 GKK67 pKa = 10.55 AKK69 pKa = 9.97 FRR71 pKa = 11.84 NPRR74 pKa = 11.84 HH75 pKa = 5.97 FDD77 pKa = 2.95 VTHH80 pKa = 6.8 PHH82 pKa = 6.82 TSTQFHH88 pKa = 7.33 PNFQGAKK95 pKa = 9.22 SSSDD99 pKa = 2.92 VKK101 pKa = 10.47 TYY103 pKa = 8.75 MEE105 pKa = 4.98 KK106 pKa = 10.86 DD107 pKa = 3.28 GDD109 pKa = 4.1 ILDD112 pKa = 4.24 HH113 pKa = 7.29 GVFQVDD119 pKa = 3.16 GRR121 pKa = 11.84 SARR124 pKa = 11.84 GGCQTANDD132 pKa = 4.46 AYY134 pKa = 10.87 AEE136 pKa = 4.61 AINAGSKK143 pKa = 9.95 AQALKK148 pKa = 10.69 VLRR151 pKa = 11.84 EE152 pKa = 3.77 LAPRR156 pKa = 11.84 DD157 pKa = 3.63 FLLQFHH163 pKa = 6.73 NLNSNLDD170 pKa = 3.64 RR171 pKa = 11.84 YY172 pKa = 9.28 FQEE175 pKa = 4.41 PPAPYY180 pKa = 9.91 VSPFSSSSFDD190 pKa = 3.41 QVPEE194 pKa = 3.95 EE195 pKa = 4.12 LEE197 pKa = 3.7 EE198 pKa = 4.13 WACEE202 pKa = 4.07 NVVDD206 pKa = 4.84 AAGRR210 pKa = 11.84 PLRR213 pKa = 11.84 PQSIVIEE220 pKa = 4.44 GDD222 pKa = 3.12 SRR224 pKa = 11.84 TGKK227 pKa = 8.52 TMWARR232 pKa = 11.84 SLGPHH237 pKa = 6.71 NYY239 pKa = 10.18 LCGHH243 pKa = 7.38 LDD245 pKa = 4.18 LSPKK249 pKa = 10.15 VYY251 pKa = 11.07 SNDD254 pKa = 2.25 AWYY257 pKa = 10.92 NVIDD261 pKa = 4.79 DD262 pKa = 4.33 VDD264 pKa = 3.73 PHH266 pKa = 6.08 FLKK269 pKa = 10.67 HH270 pKa = 5.9 FKK272 pKa = 10.69 EE273 pKa = 4.43 FMGAQRR279 pKa = 11.84 DD280 pKa = 3.81 WQSNTKK286 pKa = 9.6 YY287 pKa = 10.57 GKK289 pKa = 9.12 PVQIKK294 pKa = 10.41 GGIPTIFLCNPGPNASYY311 pKa = 11.2 KK312 pKa = 10.7 EE313 pKa = 3.97 FLDD316 pKa = 3.54 EE317 pKa = 5.43 DD318 pKa = 4.1 KK319 pKa = 11.59 NSALKK324 pKa = 10.43 SWALKK329 pKa = 9.99 NATFVFLTQPLYY341 pKa = 11.2 SGTNQSSTQGSEE353 pKa = 3.58 EE354 pKa = 4.12 SAQEE358 pKa = 4.12 EE359 pKa = 4.72 TSGPP363 pKa = 3.66
Molecular weight: 40.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.033
IPC2_protein 5.957
IPC_protein 6.046
Toseland 6.44
ProMoST 6.453
Dawson 6.376
Bjellqvist 6.351
Wikipedia 6.402
Rodwell 6.364
Grimsley 6.59
Solomon 6.376
Lehninger 6.376
Nozaki 6.649
DTASelect 6.839
Thurlkill 6.868
EMBOSS 6.839
Sillero 6.766
Patrickios 4.215
IPC_peptide 6.389
IPC2_peptide 6.693
IPC2.peptide.svr19 6.67
Protein with the highest isoelectric point:
>tr|E7BNI8|E7BNI8_9GEMI AC5 protein OS=Cotton leaf curl Allahabad virus [India:Karnal:OY81B:2005] OX=908136 GN=AC5 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 10.21 RR4 pKa = 11.84 AADD7 pKa = 3.55 IVISTPASKK16 pKa = 10.29 VRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LNFGSAYY27 pKa = 8.43 TSRR30 pKa = 11.84 AAAPIVRR37 pKa = 11.84 VTKK40 pKa = 9.87 QQAWSNRR47 pKa = 11.84 PMNRR51 pKa = 11.84 KK52 pKa = 7.98 PRR54 pKa = 11.84 MYY56 pKa = 10.68 RR57 pKa = 11.84 MYY59 pKa = 10.44 RR60 pKa = 11.84 SPDD63 pKa = 3.21 VPRR66 pKa = 11.84 GCEE69 pKa = 4.34 GPCKK73 pKa = 10.01 VQSFEE78 pKa = 4.02 SRR80 pKa = 11.84 HH81 pKa = 5.54 DD82 pKa = 3.51 VVHH85 pKa = 6.75 IGKK88 pKa = 9.41 VMCISDD94 pKa = 3.53 VTRR97 pKa = 11.84 GVGLTHH103 pKa = 7.8 RR104 pKa = 11.84 IGKK107 pKa = 8.54 RR108 pKa = 11.84 FCVKK112 pKa = 9.96 SVYY115 pKa = 10.56 VLGKK119 pKa = 9.46 IWTDD123 pKa = 3.29 EE124 pKa = 4.15 NIKK127 pKa = 9.36 TKK129 pKa = 10.64 NHH131 pKa = 5.78 TNSVMFFLVRR141 pKa = 11.84 DD142 pKa = 3.83 RR143 pKa = 11.84 RR144 pKa = 11.84 PTDD147 pKa = 3.06 KK148 pKa = 10.78 PQDD151 pKa = 3.59 FGEE154 pKa = 4.33 VFNMFDD160 pKa = 4.29 NEE162 pKa = 4.11 PSTATVKK169 pKa = 10.61 NMHH172 pKa = 7.0 RR173 pKa = 11.84 DD174 pKa = 3.48 RR175 pKa = 11.84 YY176 pKa = 9.29 QVLSKK181 pKa = 9.35 WHH183 pKa = 5.64 ATVTGGQYY191 pKa = 10.85 ASKK194 pKa = 10.24 EE195 pKa = 3.73 QALVKK200 pKa = 10.68 KK201 pKa = 9.39 FVRR204 pKa = 11.84 VNNYY208 pKa = 7.82 VVYY211 pKa = 9.96 NQQEE215 pKa = 3.8 AGKK218 pKa = 10.1 YY219 pKa = 8.23 EE220 pKa = 4.02 NHH222 pKa = 6.47 TEE224 pKa = 3.99 NALMLYY230 pKa = 7.52 MACTHH235 pKa = 7.06 ASNPVYY241 pKa = 9.86 ATLKK245 pKa = 9.47 IRR247 pKa = 11.84 IYY249 pKa = 10.64 FYY251 pKa = 11.34 DD252 pKa = 3.32 SVTNN256 pKa = 3.98
Molecular weight: 29.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.252
IPC2_protein 9.502
IPC_protein 9.663
Toseland 10.101
ProMoST 9.823
Dawson 10.321
Bjellqvist 10.014
Wikipedia 10.496
Rodwell 10.687
Grimsley 10.394
Solomon 10.335
Lehninger 10.306
Nozaki 10.131
DTASelect 9.999
Thurlkill 10.16
EMBOSS 10.511
Sillero 10.233
Patrickios 10.204
IPC_peptide 10.335
IPC2_peptide 8.858
IPC2.peptide.svr19 8.459
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1242
100
363
177.4
20.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.509 ± 1.199
2.415 ± 0.647
4.106 ± 0.825
4.267 ± 0.664
4.509 ± 0.757
4.831 ± 0.438
4.026 ± 0.648
5.395 ± 0.916
6.039 ± 0.582
8.052 ± 1.505
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.738 ± 0.667
6.039 ± 0.734
6.28 ± 0.871
4.75 ± 0.647
6.361 ± 1.039
8.696 ± 1.457
5.717 ± 0.794
6.522 ± 1.32
1.208 ± 0.099
3.543 ± 0.83
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here