Cotton leaf curl Allahabad virus [India:Karnal:OY81B:2005] 
Average proteome isoelectric point is 8.28 
Get precalculated fractions of proteins 
 
  
    Acidic  
     
   
 
  
    pI < 6.8  
     
   
 
  
    6.8-7.4  
     
   
 
  
    pI > 7.4  
     
   
 
  
    Basic  
     
   
    
 
  
    All  
     
   
 
 
Note: above files contain also dissociation constants (pKa) 
 
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 
 Summary statistics related to proteome-wise predictions 
 
Protein with the lowest isoelectric point: 
>tr|E7BNJ2|E7BNJ2_9GEMI AC4 protein OS=Cotton leaf curl Allahabad virus [India:Karnal:OY81B:2005] OX=908136 GN=AC4 PE=3 SV=1 
MM1 pKa = 7.59  PRR3 pKa = 11.84  NNSFCINAKK12 pKa = 10.45  NIFLTFPKK20 pKa = 10.37  CPIPKK25 pKa = 9.7  EE26 pKa = 3.87  QMLDD30 pKa = 2.9  ILSNISCPSDD40 pKa = 3.09  KK41 pKa = 11.04  LFIRR45 pKa = 11.84  VAQEE49 pKa = 3.46  KK50 pKa = 9.49  HH51 pKa = 5.19  QDD53 pKa = 3.38  GSLHH57 pKa = 5.16  IHH59 pKa = 6.91  ALIQFKK65 pKa = 10.83  GKK67 pKa = 10.55  AKK69 pKa = 9.97  FRR71 pKa = 11.84  NPRR74 pKa = 11.84  HH75 pKa = 5.97  FDD77 pKa = 2.95  VTHH80 pKa = 6.8  PHH82 pKa = 6.82  TSTQFHH88 pKa = 7.33  PNFQGAKK95 pKa = 9.22  SSSDD99 pKa = 2.92  VKK101 pKa = 10.47  TYY103 pKa = 8.75  MEE105 pKa = 4.98  KK106 pKa = 10.86  DD107 pKa = 3.28  GDD109 pKa = 4.1  ILDD112 pKa = 4.24  HH113 pKa = 7.29  GVFQVDD119 pKa = 3.16  GRR121 pKa = 11.84  SARR124 pKa = 11.84  GGCQTANDD132 pKa = 4.46  AYY134 pKa = 10.87  AEE136 pKa = 4.61  AINAGSKK143 pKa = 9.95  AQALKK148 pKa = 10.69  VLRR151 pKa = 11.84  EE152 pKa = 3.77  LAPRR156 pKa = 11.84  DD157 pKa = 3.63  FLLQFHH163 pKa = 6.73  NLNSNLDD170 pKa = 3.64  RR171 pKa = 11.84  YY172 pKa = 9.28  FQEE175 pKa = 4.41  PPAPYY180 pKa = 9.91  VSPFSSSSFDD190 pKa = 3.41  QVPEE194 pKa = 3.95  EE195 pKa = 4.12  LEE197 pKa = 3.7  EE198 pKa = 4.13  WACEE202 pKa = 4.07  NVVDD206 pKa = 4.84  AAGRR210 pKa = 11.84  PLRR213 pKa = 11.84  PQSIVIEE220 pKa = 4.44  GDD222 pKa = 3.12  SRR224 pKa = 11.84  TGKK227 pKa = 8.52  TMWARR232 pKa = 11.84  SLGPHH237 pKa = 6.71  NYY239 pKa = 10.18  LCGHH243 pKa = 7.38  LDD245 pKa = 4.18  LSPKK249 pKa = 10.15  VYY251 pKa = 11.07  SNDD254 pKa = 2.25  AWYY257 pKa = 10.92  NVIDD261 pKa = 4.79  DD262 pKa = 4.33  VDD264 pKa = 3.73  PHH266 pKa = 6.08  FLKK269 pKa = 10.67  HH270 pKa = 5.9  FKK272 pKa = 10.69  EE273 pKa = 4.43  FMGAQRR279 pKa = 11.84  DD280 pKa = 3.81  WQSNTKK286 pKa = 9.6  YY287 pKa = 10.57  GKK289 pKa = 9.12  PVQIKK294 pKa = 10.41  GGIPTIFLCNPGPNASYY311 pKa = 11.2  KK312 pKa = 10.7  EE313 pKa = 3.97  FLDD316 pKa = 3.54  EE317 pKa = 5.43  DD318 pKa = 4.1  KK319 pKa = 11.59  NSALKK324 pKa = 10.43  SWALKK329 pKa = 9.99  NATFVFLTQPLYY341 pKa = 11.2  SGTNQSSTQGSEE353 pKa = 3.58  EE354 pKa = 4.12  SAQEE358 pKa = 4.12  EE359 pKa = 4.72  TSGPP363 pKa = 3.66   
 Molecular weight: 40.7 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  6.033 
IPC2_protein 5.957 
IPC_protein 6.046 
Toseland    6.44 
ProMoST     6.453 
Dawson      6.376 
Bjellqvist  6.351 
Wikipedia   6.402 
Rodwell     6.364 
Grimsley    6.59 
Solomon     6.376 
Lehninger   6.376 
Nozaki      6.649 
DTASelect   6.839 
Thurlkill   6.868 
EMBOSS      6.839 
Sillero     6.766 
Patrickios  4.215 
IPC_peptide 6.389 
IPC2_peptide  6.693 
IPC2.peptide.svr19  6.67 
 Protein with the highest isoelectric point: 
>tr|E7BNI8|E7BNI8_9GEMI AC5 protein OS=Cotton leaf curl Allahabad virus [India:Karnal:OY81B:2005] OX=908136 GN=AC5 PE=4 SV=1 
MM1 pKa = 7.69  SKK3 pKa = 10.21  RR4 pKa = 11.84  AADD7 pKa = 3.55  IVISTPASKK16 pKa = 10.29  VRR18 pKa = 11.84  RR19 pKa = 11.84  RR20 pKa = 11.84  LNFGSAYY27 pKa = 8.43  TSRR30 pKa = 11.84  AAAPIVRR37 pKa = 11.84  VTKK40 pKa = 9.87  QQAWSNRR47 pKa = 11.84  PMNRR51 pKa = 11.84  KK52 pKa = 7.98  PRR54 pKa = 11.84  MYY56 pKa = 10.68  RR57 pKa = 11.84  MYY59 pKa = 10.44  RR60 pKa = 11.84  SPDD63 pKa = 3.21  VPRR66 pKa = 11.84  GCEE69 pKa = 4.34  GPCKK73 pKa = 10.01  VQSFEE78 pKa = 4.02  SRR80 pKa = 11.84  HH81 pKa = 5.54  DD82 pKa = 3.51  VVHH85 pKa = 6.75  IGKK88 pKa = 9.41  VMCISDD94 pKa = 3.53  VTRR97 pKa = 11.84  GVGLTHH103 pKa = 7.8  RR104 pKa = 11.84  IGKK107 pKa = 8.54  RR108 pKa = 11.84  FCVKK112 pKa = 9.96  SVYY115 pKa = 10.56  VLGKK119 pKa = 9.46  IWTDD123 pKa = 3.29  EE124 pKa = 4.15  NIKK127 pKa = 9.36  TKK129 pKa = 10.64  NHH131 pKa = 5.78  TNSVMFFLVRR141 pKa = 11.84  DD142 pKa = 3.83  RR143 pKa = 11.84  RR144 pKa = 11.84  PTDD147 pKa = 3.06  KK148 pKa = 10.78  PQDD151 pKa = 3.59  FGEE154 pKa = 4.33  VFNMFDD160 pKa = 4.29  NEE162 pKa = 4.11  PSTATVKK169 pKa = 10.61  NMHH172 pKa = 7.0  RR173 pKa = 11.84  DD174 pKa = 3.48  RR175 pKa = 11.84  YY176 pKa = 9.29  QVLSKK181 pKa = 9.35  WHH183 pKa = 5.64  ATVTGGQYY191 pKa = 10.85  ASKK194 pKa = 10.24  EE195 pKa = 3.73  QALVKK200 pKa = 10.68  KK201 pKa = 9.39  FVRR204 pKa = 11.84  VNNYY208 pKa = 7.82  VVYY211 pKa = 9.96  NQQEE215 pKa = 3.8  AGKK218 pKa = 10.1  YY219 pKa = 8.23  EE220 pKa = 4.02  NHH222 pKa = 6.47  TEE224 pKa = 3.99  NALMLYY230 pKa = 7.52  MACTHH235 pKa = 7.06  ASNPVYY241 pKa = 9.86  ATLKK245 pKa = 9.47  IRR247 pKa = 11.84  IYY249 pKa = 10.64  FYY251 pKa = 11.34  DD252 pKa = 3.32  SVTNN256 pKa = 3.98   
 Molecular weight: 29.52 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.252 
IPC2_protein 9.502 
IPC_protein 9.663 
Toseland    10.101 
ProMoST     9.823 
Dawson      10.321 
Bjellqvist  10.014 
Wikipedia   10.496 
Rodwell     10.687 
Grimsley    10.394 
Solomon     10.335 
Lehninger   10.306 
Nozaki      10.131 
DTASelect   9.999 
Thurlkill   10.16 
EMBOSS      10.511 
Sillero     10.233 
Patrickios  10.204 
IPC_peptide 10.335 
IPC2_peptide  8.858 
IPC2.peptide.svr19  8.459 
  
 
Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try ESI  
     
   
 
  
    ChTry ESI  
     
   
 
  
    ArgC ESI  
     
   
 
  
    LysN ESI  
     
   
 
  
    TryLysC ESI  
     
   
 
  
    Try MALDI  
     
   
 
  
    ChTry MALDI  
     
   
 
  
    ArgC MALDI  
     
   
 
  
    LysN MALDI  
     
   
 
  
    TryLysC MALDI  
     
   
 
  
    Try LTQ  
     
   
 
  
    ChTry LTQ  
     
   
 
  
    ArgC LTQ  
     
   
 
  
    LysN LTQ  
     
   
 
  
    TryLysC LTQ  
     
   
 
  
    Try MSlow  
     
   
 
  
    ChTry MSlow  
     
   
 
  
    ArgC MSlow  
     
   
 
  
    LysN MSlow  
     
   
 
  
    TryLysC MSlow  
     
   
 
  
    Try MShigh  
     
   
 
  
    ChTry MShigh  
     
   
 
  
    ArgC MShigh  
     
   
 
  
    LysN MShigh  
     
   
 
  
    TryLysC MShigh  
     
   
   
  
                       
General Statistics 
    
      
        Number of major isoforms
 
        Number of additional isoforms
 
        Number of all proteins
         
        Number of amino acids
 
        Min. Seq. Length
 
        Max. Seq. Length
 
        Avg. Seq. Length
 
        Avg. Mol. Weight
 
       
     
    
      
        7 
 
        
        0
 
        
        7 
         
        1242
 
        100
 
        363
 
        177.4
 
        20.29
 
                
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
 
        Cys
 
        Asp
 
        Glu
 
        Phe
 
        Gly
 
        His
 
        Ile
 
        Lys
 
        Leu
 
       
     
    
                 
        4.509 ± 1.199
2.415 ± 0.647
 
        4.106 ± 0.825
4.267 ± 0.664
 
        4.509 ± 0.757
4.831 ± 0.438
 
        4.026 ± 0.648
5.395 ± 0.916
       
        6.039 ± 0.582
8.052 ± 1.505
         
                
     
  
  
  
      
          
        Met
         
        Asn
 
        Gln
 
        Pro
         
        Arg
 
        Ser
 
        Thr
 
        Val
 
        Trp
 
        Tyr
  
       
     
    
                 
        2.738 ± 0.667
6.039 ± 0.734
 
        6.28 ± 0.871
4.75 ± 0.647
 
        6.361 ± 1.039
8.696 ± 1.457
 
        5.717 ± 0.794
6.522 ± 1.32
       
        1.208 ± 0.099
3.543 ± 0.83
         
                
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level 
Most of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here