Paenibacillus anaericanus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5415 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S1BG44|A0A3S1BG44_9BACL Uncharacterized protein OS=Paenibacillus anaericanus OX=170367 GN=EJP82_26755 PE=4 SV=1
MM1 pKa = 7.32SAIDD5 pKa = 3.53AQQRR9 pKa = 11.84KK10 pKa = 8.71VMDD13 pKa = 3.9SYY15 pKa = 12.09GKK17 pKa = 9.86IPLTFVEE24 pKa = 4.79NCGQTQANIRR34 pKa = 11.84YY35 pKa = 5.35YY36 pKa = 9.65TKK38 pKa = 9.9RR39 pKa = 11.84LGYY42 pKa = 10.09GVYY45 pKa = 8.33FTPNEE50 pKa = 3.63AAFFFRR56 pKa = 11.84DD57 pKa = 3.17NSRR60 pKa = 11.84QEE62 pKa = 4.06NVEE65 pKa = 3.71QSLFLQFPNANPEE78 pKa = 3.99VKK80 pKa = 10.73LEE82 pKa = 3.87GRR84 pKa = 11.84VKK86 pKa = 10.72GSEE89 pKa = 3.96KK90 pKa = 10.88VNFLIGNDD98 pKa = 3.39PTKK101 pKa = 10.03WHH103 pKa = 6.89TGLSTYY109 pKa = 10.16QEE111 pKa = 3.99VVYY114 pKa = 10.7RR115 pKa = 11.84EE116 pKa = 4.01LWSGVDD122 pKa = 3.34LVFYY126 pKa = 10.56EE127 pKa = 4.91GEE129 pKa = 4.25GKK131 pKa = 10.48LKK133 pKa = 10.64YY134 pKa = 10.22DD135 pKa = 5.23VILQPHH141 pKa = 6.69AKK143 pKa = 10.23VNDD146 pKa = 3.56VQLTYY151 pKa = 10.56HH152 pKa = 6.42GSDD155 pKa = 3.29YY156 pKa = 11.32LSLNEE161 pKa = 4.36EE162 pKa = 4.11GDD164 pKa = 4.2LLVHH168 pKa = 6.48TSLGVWTEE176 pKa = 3.98KK177 pKa = 10.87QPVSYY182 pKa = 11.02QEE184 pKa = 3.95INGKK188 pKa = 9.51KK189 pKa = 9.74VFVEE193 pKa = 4.1SRR195 pKa = 11.84FVLKK199 pKa = 9.54EE200 pKa = 3.82TDD202 pKa = 3.39QGDD205 pKa = 3.63QVYY208 pKa = 9.46GFEE211 pKa = 4.58MGQDD215 pKa = 3.67YY216 pKa = 11.35NPDD219 pKa = 3.44YY220 pKa = 11.31VLVIDD225 pKa = 5.67PILMYY230 pKa = 10.16STFLGGSVGPDD241 pKa = 2.94AGQGIAVDD249 pKa = 3.95TAGNAYY255 pKa = 8.37VTGFTAGSFPTTSGAYY271 pKa = 6.66QTIFGGDD278 pKa = 3.07SDD280 pKa = 4.96VFITKK285 pKa = 9.3MNSTGRR291 pKa = 11.84APIYY295 pKa = 9.31STYY298 pKa = 10.82LGGSGTDD305 pKa = 3.12VGQGITVDD313 pKa = 3.69TSGNAYY319 pKa = 7.83VTGYY323 pKa = 9.2TNSNDD328 pKa = 3.09FPTTVGAFQISLGGVGATNAFVTKK352 pKa = 10.54LNATGTLALYY362 pKa = 8.51STYY365 pKa = 10.92LGGDD369 pKa = 3.5TVDD372 pKa = 2.95IGYY375 pKa = 10.35GIAIDD380 pKa = 3.88ASGNAYY386 pKa = 8.38VTGSTTSGNYY396 pKa = 9.19PVTAGIFQGTLGGPGATNAFVTKK419 pKa = 10.61LNATGTAPLVYY430 pKa = 9.14STYY433 pKa = 11.04LGGTVSDD440 pKa = 3.83VGQGIAVDD448 pKa = 4.0ALGNAYY454 pKa = 8.32VTGSTTSTDD463 pKa = 3.87FPMVAGSVQTTYY475 pKa = 11.03GGSGDD480 pKa = 4.08AFVTKK485 pKa = 10.67LNATATAPLIYY496 pKa = 9.79STFLGGNGLDD506 pKa = 2.93IGYY509 pKa = 10.15SIGIDD514 pKa = 3.21AAGNAYY520 pKa = 9.54IAGFTDD526 pKa = 3.38SANYY530 pKa = 8.85PVEE533 pKa = 4.55GQIQPYY539 pKa = 9.73GGAGDD544 pKa = 4.3AMVSKK549 pKa = 10.51LNPAGTGLVYY559 pKa = 9.82STYY562 pKa = 10.73LGGIARR568 pKa = 11.84DD569 pKa = 3.44EE570 pKa = 3.97ARR572 pKa = 11.84AIVVDD577 pKa = 3.88PAGNAYY583 pKa = 8.82LTGNTEE589 pKa = 3.84SSDD592 pKa = 3.71YY593 pKa = 9.47PLSVGAIQTGLLGVRR608 pKa = 11.84DD609 pKa = 3.8VFITLFNTAGNALVYY624 pKa = 9.45STYY627 pKa = 10.98LGGTGVEE634 pKa = 4.2EE635 pKa = 4.37GLGIAVDD642 pKa = 4.24SNFNLYY648 pKa = 8.15VTGSTTSLNFPITPGAFQTSLPGLPGSGFVTKK680 pKa = 10.31IGTPIQLLGEE690 pKa = 4.34TGATGDD696 pKa = 4.18PGVTGPTGPTGDD708 pKa = 3.53TGATGEE714 pKa = 4.28TGATGEE720 pKa = 4.28TGATGEE726 pKa = 4.37TGATGDD732 pKa = 3.54TGATGEE738 pKa = 4.38TGATGDD744 pKa = 3.54TGATGEE750 pKa = 4.38TGATGDD756 pKa = 3.73TGPTGGTGATGDD768 pKa = 3.45TGATGEE774 pKa = 4.38TGATGDD780 pKa = 3.73TGPTGGTGATGDD792 pKa = 3.49TGATGGTGATGGTGATGEE810 pKa = 4.28TGATGDD816 pKa = 3.67TGATGDD822 pKa = 3.89TGATGATGEE831 pKa = 4.41TGATGDD837 pKa = 3.54TGATGEE843 pKa = 4.38TGATGDD849 pKa = 3.4TGAIGGTGATGDD861 pKa = 3.58TGATGDD867 pKa = 3.63TGATGEE873 pKa = 4.25TGATGGTGAAGEE885 pKa = 4.27TGATGEE891 pKa = 4.37TGATGDD897 pKa = 3.54TGATGEE903 pKa = 4.38TGATGDD909 pKa = 3.54TGATGEE915 pKa = 4.38TGATGDD921 pKa = 3.73TGPTGGTGATGDD933 pKa = 3.49TGATGGTGATGGTGATGEE951 pKa = 4.28TGATGDD957 pKa = 3.54TGATGEE963 pKa = 4.28TGEE966 pKa = 4.42TGATGEE972 pKa = 4.37TGATGDD978 pKa = 3.54TGATGEE984 pKa = 4.38TGATGDD990 pKa = 3.4TGAIGGTGATGDD1002 pKa = 3.58TGATGDD1008 pKa = 3.63TGATGEE1014 pKa = 4.25TGATGGTGAAGEE1026 pKa = 4.27TGATGEE1032 pKa = 4.41TGATGG1037 pKa = 3.17

Molecular weight: 101.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A433YB49|A0A433YB49_9BACL CBS domain-containing protein OS=Paenibacillus anaericanus OX=170367 GN=EJP82_07905 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84PTFKK6 pKa = 10.82PNVSKK11 pKa = 10.8RR12 pKa = 11.84KK13 pKa = 8.95KK14 pKa = 8.25VHH16 pKa = 5.49GFRR19 pKa = 11.84KK20 pKa = 9.94RR21 pKa = 11.84MSTASGRR28 pKa = 11.84KK29 pKa = 8.42VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.2GRR39 pKa = 11.84KK40 pKa = 8.79VLSAA44 pKa = 4.05

Molecular weight: 5.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5415

0

5415

1728414

16

2567

319.2

35.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.011 ± 0.036

0.788 ± 0.011

5.286 ± 0.028

6.819 ± 0.038

4.248 ± 0.026

7.204 ± 0.041

2.006 ± 0.017

7.526 ± 0.035

5.769 ± 0.026

10.011 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.827 ± 0.017

4.36 ± 0.024

3.729 ± 0.024

3.78 ± 0.018

4.477 ± 0.028

6.701 ± 0.033

5.587 ± 0.04

7.003 ± 0.026

1.226 ± 0.014

3.642 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski