Streptococcus phage Javan179

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A1M8|A0A4D6A1M8_9CAUD Mobile element protein OS=Streptococcus phage Javan179 OX=2548026 GN=Javan179_0005 PE=4 SV=1
MM1 pKa = 7.82LIYY4 pKa = 10.15PATFTQDD11 pKa = 2.07TDD13 pKa = 4.11YY14 pKa = 11.77IMVTFPDD21 pKa = 3.7VPEE24 pKa = 5.7AITQGKK30 pKa = 9.19DD31 pKa = 3.12FQEE34 pKa = 4.67AYY36 pKa = 10.14EE37 pKa = 4.16MAVEE41 pKa = 4.15VLGFALEE48 pKa = 5.25DD49 pKa = 3.43YY50 pKa = 10.49TDD52 pKa = 3.75YY53 pKa = 11.24PKK55 pKa = 10.97ASSVSDD61 pKa = 3.46LKK63 pKa = 10.86EE64 pKa = 3.61QYY66 pKa = 9.91RR67 pKa = 11.84DD68 pKa = 3.09SDD70 pKa = 3.48IALIGIDD77 pKa = 3.72MIAYY81 pKa = 6.12MKK83 pKa = 10.17KK84 pKa = 8.24YY85 pKa = 9.68HH86 pKa = 5.89SKK88 pKa = 10.45KK89 pKa = 8.6VRR91 pKa = 11.84KK92 pKa = 9.32NVTIPEE98 pKa = 4.33WLNKK102 pKa = 9.73AAEE105 pKa = 4.23DD106 pKa = 3.77EE107 pKa = 4.32NLNFSQVLTEE117 pKa = 4.09ALEE120 pKa = 4.63LKK122 pKa = 10.44LQAA125 pKa = 4.95

Molecular weight: 14.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A1N9|A0A4D6A1N9_9CAUD Terminase large subunit OS=Streptococcus phage Javan179 OX=2548026 GN=Javan179_0025 PE=4 SV=1
MM1 pKa = 7.55NKK3 pKa = 10.03RR4 pKa = 11.84IKK6 pKa = 10.22KK7 pKa = 9.09KK8 pKa = 10.34RR9 pKa = 11.84KK10 pKa = 9.22LEE12 pKa = 4.06TAVVMLVAEE21 pKa = 4.41NAMQAEE27 pKa = 4.45ALRR30 pKa = 11.84NQNRR34 pKa = 11.84QIAEE38 pKa = 4.04LRR40 pKa = 11.84SIIQQNAQATNRR52 pKa = 11.84EE53 pKa = 3.81FAAVKK58 pKa = 10.35SATLDD63 pKa = 3.22NQAAIKK69 pKa = 10.36KK70 pKa = 9.93IGDD73 pKa = 3.26EE74 pKa = 4.09VYY76 pKa = 10.81YY77 pKa = 10.27IKK79 pKa = 10.7QNYY82 pKa = 7.29KK83 pKa = 10.42RR84 pKa = 11.84KK85 pKa = 8.22WRR87 pKa = 11.84KK88 pKa = 8.88

Molecular weight: 10.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

12023

42

1219

222.6

25.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.12 ± 0.608

0.557 ± 0.114

6.762 ± 0.314

7.12 ± 0.307

3.851 ± 0.272

6.496 ± 0.347

1.406 ± 0.148

6.97 ± 0.26

8.858 ± 0.3

8.65 ± 0.261

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.545 ± 0.18

5.431 ± 0.206

2.778 ± 0.195

3.951 ± 0.242

4.491 ± 0.212

6.03 ± 0.289

5.814 ± 0.18

6.196 ± 0.279

1.156 ± 0.126

3.818 ± 0.289
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski