Streptococcus virus ALQ132
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C9W9J9|C9W9J9_9CAUD Uncharacterized protein OS=Streptococcus virus ALQ132 OX=682370 PE=4 SV=1
MM1 pKa = 7.12 VNWVDD6 pKa = 4.58 KK7 pKa = 11.1 DD8 pKa = 4.24 GNDD11 pKa = 3.97 LPDD14 pKa = 4.26 GADD17 pKa = 3.29 QEE19 pKa = 5.13 FKK21 pKa = 11.28 AGMFFSFAGDD31 pKa = 3.4 EE32 pKa = 4.36 TNITDD37 pKa = 3.65 TGEE40 pKa = 3.72 GGYY43 pKa = 10.63 YY44 pKa = 9.66 GGYY47 pKa = 8.9 YY48 pKa = 9.56 FRR50 pKa = 11.84 KK51 pKa = 9.96 FEE53 pKa = 4.13 FGQFGTVWLSCWNKK67 pKa = 10.66 DD68 pKa = 3.89 DD69 pKa = 5.14 LVNYY73 pKa = 7.38 YY74 pKa = 8.26 QQ75 pKa = 4.22
Molecular weight: 8.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.748
IPC2_protein 3.897
IPC_protein 3.834
Toseland 3.617
ProMoST 4.024
Dawson 3.834
Bjellqvist 3.999
Wikipedia 3.808
Rodwell 3.656
Grimsley 3.528
Solomon 3.808
Lehninger 3.77
Nozaki 3.961
DTASelect 4.202
Thurlkill 3.694
EMBOSS 3.808
Sillero 3.948
Patrickios 0.769
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.848
Protein with the highest isoelectric point:
>tr|C9W9L2|C9W9L2_9CAUD Uncharacterized protein OS=Streptococcus virus ALQ132 OX=682370 PE=4 SV=1
MM1 pKa = 7.55 GLTVTCCDD9 pKa = 3.57 NLRR12 pKa = 11.84 LLSNHH17 pKa = 5.68 NHH19 pKa = 5.11 YY20 pKa = 11.07 CCFLRR25 pKa = 11.84 LVFVPIKK32 pKa = 10.46 IEE34 pKa = 3.61 IMPINWLIFSWVKK47 pKa = 9.88 KK48 pKa = 9.58 IKK50 pKa = 10.41 RR51 pKa = 11.84 FLSIFNPTYY60 pKa = 10.42 RR61 pKa = 4.35
Molecular weight: 7.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.224
IPC2_protein 8.873
IPC_protein 8.931
Toseland 9.443
ProMoST 9.575
Dawson 9.721
Bjellqvist 9.619
Wikipedia 9.867
Rodwell 9.955
Grimsley 9.721
Solomon 9.853
Lehninger 9.838
Nozaki 9.867
DTASelect 9.487
Thurlkill 9.619
EMBOSS 9.882
Sillero 9.794
Patrickios 7.468
IPC_peptide 9.838
IPC2_peptide 8.931
IPC2.peptide.svr19 7.917
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
44
0
44
10903
46
1534
247.8
27.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.457 ± 1.267
0.633 ± 0.159
6.301 ± 0.543
6.374 ± 0.773
4.292 ± 0.188
7.053 ± 0.572
1.238 ± 0.194
6.86 ± 0.447
7.979 ± 0.687
7.677 ± 0.356
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.559 ± 0.344
5.76 ± 0.468
2.807 ± 0.267
3.715 ± 0.299
4.338 ± 0.457
6.86 ± 0.69
6.457 ± 0.402
6.512 ± 0.253
1.202 ± 0.203
3.926 ± 0.506
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here