Cucumber green mottle mosaic virus (strain watermelon SH) (CGMMV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Virgaviridae; Tobamovirus; Cucumber green mottle mosaic virus

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P69514|RDRP_CGMVS Replicase large subunit OS=Cucumber green mottle mosaic virus (strain watermelon SH) OX=12236 PE=3 SV=1
MM1 pKa = 8.08AYY3 pKa = 10.67NPITPSKK10 pKa = 10.34LIAFSASYY18 pKa = 11.04VPVRR22 pKa = 11.84TLLNFLVASQGTAFQTQAGRR42 pKa = 11.84DD43 pKa = 3.58SFRR46 pKa = 11.84EE47 pKa = 4.08SLSALPSSVVDD58 pKa = 3.42INSRR62 pKa = 11.84FPDD65 pKa = 3.21AGFYY69 pKa = 10.97AFLNGPVLRR78 pKa = 11.84PIFVSLLSSTDD89 pKa = 2.69TRR91 pKa = 11.84NRR93 pKa = 11.84VIEE96 pKa = 4.2VVDD99 pKa = 3.77PSNPTTAEE107 pKa = 3.62SLNAVKK113 pKa = 9.56RR114 pKa = 11.84TDD116 pKa = 3.77DD117 pKa = 3.25ASTAARR123 pKa = 11.84AEE125 pKa = 3.78IDD127 pKa = 3.48NLIEE131 pKa = 4.58SISKK135 pKa = 10.6GFDD138 pKa = 3.05VYY140 pKa = 11.54DD141 pKa = 3.65RR142 pKa = 11.84ASFEE146 pKa = 4.19AAFSVVWSEE155 pKa = 3.89ATTSKK160 pKa = 11.24AA161 pKa = 3.27

Molecular weight: 17.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P69474|CAPSD_CGMVS Capsid protein OS=Cucumber green mottle mosaic virus (strain watermelon SH) OX=12236 GN=CP PE=3 SV=2
MM1 pKa = 7.34SLSKK5 pKa = 11.08VSVEE9 pKa = 4.11NSLKK13 pKa = 10.18PEE15 pKa = 4.01KK16 pKa = 9.78FVKK19 pKa = 10.4ISWVDD24 pKa = 3.32KK25 pKa = 10.65LLPNYY30 pKa = 10.05FSILKK35 pKa = 9.33YY36 pKa = 10.92LSITDD41 pKa = 3.82FSVVKK46 pKa = 9.92AQSYY50 pKa = 9.68EE51 pKa = 3.98SLVPVKK57 pKa = 10.46LLRR60 pKa = 11.84GVDD63 pKa = 3.97LTKK66 pKa = 10.58HH67 pKa = 6.35LYY69 pKa = 8.19VTLLGVVVSGVWNVPEE85 pKa = 4.23SCRR88 pKa = 11.84GGATVALVDD97 pKa = 3.72TRR99 pKa = 11.84MHH101 pKa = 6.14SVAEE105 pKa = 3.95GTICKK110 pKa = 10.07FSAPATVRR118 pKa = 11.84EE119 pKa = 4.05FSVRR123 pKa = 11.84FIPNYY128 pKa = 9.53PVVAADD134 pKa = 3.99ALRR137 pKa = 11.84DD138 pKa = 3.58PWSLFVRR145 pKa = 11.84LSNVGIKK152 pKa = 10.52DD153 pKa = 3.42GFHH156 pKa = 7.1PLTLEE161 pKa = 4.28VACLVATTNSIIKK174 pKa = 9.78KK175 pKa = 8.24GLRR178 pKa = 11.84ASVVEE183 pKa = 4.38SVVSSDD189 pKa = 3.3QSIVLDD195 pKa = 3.68SLSEE199 pKa = 4.04KK200 pKa = 10.64VEE202 pKa = 3.99PFFDD206 pKa = 3.71KK207 pKa = 11.19VPISAAVMARR217 pKa = 11.84DD218 pKa = 3.19PSYY221 pKa = 10.88RR222 pKa = 11.84SRR224 pKa = 11.84SQSVGGRR231 pKa = 11.84GKK233 pKa = 10.21RR234 pKa = 11.84HH235 pKa = 5.83SKK237 pKa = 9.55PPNRR241 pKa = 11.84RR242 pKa = 11.84LDD244 pKa = 3.66SASEE248 pKa = 4.06EE249 pKa = 4.23SSSVSFEE256 pKa = 5.18DD257 pKa = 5.04GLQSDD262 pKa = 4.21HH263 pKa = 5.96TT264 pKa = 4.23

Molecular weight: 28.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2073

161

1648

691.0

77.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.657 ± 1.299

1.737 ± 0.523

6.561 ± 0.452

4.92 ± 0.135

5.74 ± 0.476

4.679 ± 0.348

2.315 ± 0.617

5.596 ± 0.667

6.078 ± 0.811

8.924 ± 0.321

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.315 ± 0.66

4.245 ± 0.48

4.197 ± 0.58

2.412 ± 0.366

5.162 ± 0.354

9.358 ± 2.264

5.499 ± 0.493

9.165 ± 1.426

0.965 ± 0.078

3.425 ± 0.51
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski