Caulobacter sp. Root1455

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales; Caulobacteraceae; Caulobacter; unclassified Caulobacter

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4531 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q7UF48|A0A0Q7UF48_9CAUL Methionyl-tRNA formyltransferase OS=Caulobacter sp. Root1455 OX=1736465 GN=fmt PE=3 SV=1
MM1 pKa = 6.55TTLTVGDD8 pKa = 4.44LALIGYY14 pKa = 8.84SADD17 pKa = 3.49TAGKK21 pKa = 9.52SFSFVLLQAVDD32 pKa = 3.6AGTVISFTDD41 pKa = 4.26DD42 pKa = 2.46GWLAAGGFRR51 pKa = 11.84TGEE54 pKa = 4.05GVFTYY59 pKa = 7.73TAPAGGAAAGTVITVTGLTGSLNPSTSGDD88 pKa = 3.54QIIAFQGPLSAAATPLFALDD108 pKa = 4.01FADD111 pKa = 5.49GNATYY116 pKa = 10.46AADD119 pKa = 3.72ASNSNTSAVPTGLATGSTALAFGTDD144 pKa = 2.82NGAYY148 pKa = 10.21VGTTTGTKK156 pKa = 10.12AAILSAIANSANWSLDD172 pKa = 2.98DD173 pKa = 4.87ANPVAYY179 pKa = 8.08KK180 pKa = 10.3TGFTVTDD187 pKa = 3.66GGVPSANVSISDD199 pKa = 3.57VSLTEE204 pKa = 4.25GDD206 pKa = 3.7AGTQVMTFTVTRR218 pKa = 11.84TNTTGAFTVDD228 pKa = 3.48YY229 pKa = 8.63ATADD233 pKa = 3.57GSAHH237 pKa = 6.34VGDD240 pKa = 5.69DD241 pKa = 3.36YY242 pKa = 12.19AEE244 pKa = 4.01AHH246 pKa = 5.84GTLTFAAGGPASQTISVTLNGDD268 pKa = 3.32ATPEE272 pKa = 4.08PNEE275 pKa = 4.02TFAVNLSNLVVASGAAALIDD295 pKa = 3.61AVGQATIVNDD305 pKa = 3.63DD306 pKa = 4.0FAPVAIYY313 pKa = 9.87DD314 pKa = 3.69IQGAGHH320 pKa = 6.23VSAYY324 pKa = 10.44DD325 pKa = 3.57GQTVSTQGVVTAIDD339 pKa = 3.67TTGSRR344 pKa = 11.84GFWIQDD350 pKa = 3.17ATGDD354 pKa = 4.03GDD356 pKa = 4.86DD357 pKa = 4.03ATSDD361 pKa = 3.29AVFVFTNAIPTVHH374 pKa = 6.84VGDD377 pKa = 4.6LVQVTGTVDD386 pKa = 3.31EE387 pKa = 4.71YY388 pKa = 11.69NGGVATNLSITEE400 pKa = 3.87ITAPTISVIGTGTITATVLGAGGRR424 pKa = 11.84AIPTTVIDD432 pKa = 4.84DD433 pKa = 3.83DD434 pKa = 4.71HH435 pKa = 6.53LTSFDD440 pKa = 3.74PAADD444 pKa = 3.28GVDD447 pKa = 4.1FYY449 pKa = 11.63EE450 pKa = 4.58SVEE453 pKa = 4.12GMVVTLHH460 pKa = 7.14DD461 pKa = 4.3AQSVGSTSQGQTWVVADD478 pKa = 4.29NGADD482 pKa = 3.36ASGLNSRR489 pKa = 11.84GGVTVSDD496 pKa = 3.73GDD498 pKa = 3.96NNPEE502 pKa = 4.47RR503 pKa = 11.84ILVYY507 pKa = 10.47ADD509 pKa = 3.47SGVNPGFSAGYY520 pKa = 10.15VLGDD524 pKa = 3.59HH525 pKa = 6.83LGDD528 pKa = 3.44VTGVVSYY535 pKa = 10.24FGGEE539 pKa = 4.22YY540 pKa = 10.13EE541 pKa = 4.32VLATSVQNTTSGGTLPLEE559 pKa = 4.47TTSLAGDD566 pKa = 4.09ASHH569 pKa = 7.19LAIGAYY575 pKa = 9.23NLEE578 pKa = 4.79NISPVDD584 pKa = 3.62PDD586 pKa = 3.72AKK588 pKa = 10.28FAALAADD595 pKa = 3.66IAHH598 pKa = 6.71NLGAPDD604 pKa = 3.31ILGVEE609 pKa = 5.12EE610 pKa = 4.36IQDD613 pKa = 3.39ADD615 pKa = 3.76GAGNGTNYY623 pKa = 10.63SGAATLNKK631 pKa = 9.82LVAAIEE637 pKa = 4.29AAGGPHH643 pKa = 5.95YY644 pKa = 10.65SWVEE648 pKa = 3.61IAPTANNSTGGEE660 pKa = 4.27SNGNIRR666 pKa = 11.84NAFLYY671 pKa = 10.75RR672 pKa = 11.84EE673 pKa = 4.16DD674 pKa = 3.49RR675 pKa = 11.84VDD677 pKa = 3.63YY678 pKa = 11.32VEE680 pKa = 4.77GSARR684 pKa = 11.84LIQDD688 pKa = 3.22ATGSTDD694 pKa = 2.91AFHH697 pKa = 7.63NSRR700 pKa = 11.84NPLAAEE706 pKa = 4.22FVFHH710 pKa = 7.09GEE712 pKa = 3.9TVTAIDD718 pKa = 3.18VHH720 pKa = 6.79NYY722 pKa = 9.55SRR724 pKa = 11.84GGSDD728 pKa = 3.51PLFGANQPASNNGDD742 pKa = 3.63DD743 pKa = 4.27RR744 pKa = 11.84RR745 pKa = 11.84ADD747 pKa = 3.34QSTAVHH753 pKa = 6.97DD754 pKa = 4.14YY755 pKa = 9.6VTTLLAADD763 pKa = 4.5PDD765 pKa = 3.64AHH767 pKa = 5.77ITVMGDD773 pKa = 2.96FNAYY777 pKa = 10.05YY778 pKa = 10.24YY779 pKa = 9.73EE780 pKa = 4.03QSLTLLEE787 pKa = 4.72ANGDD791 pKa = 4.07LYY793 pKa = 11.74NLARR797 pKa = 11.84TLSAEE802 pKa = 3.72EE803 pKa = 4.1RR804 pKa = 11.84YY805 pKa = 10.55SYY807 pKa = 10.68IFEE810 pKa = 4.73GNAQQIDD817 pKa = 4.06NLLVSQSLKK826 pKa = 11.02DD827 pKa = 3.34GAVFDD832 pKa = 5.26NIHH835 pKa = 6.82LNTGQAAIDD844 pKa = 3.62QPTDD848 pKa = 2.9HH849 pKa = 7.42DD850 pKa = 5.64AILALLSINTAPVATLDD867 pKa = 3.51GTFTVSEE874 pKa = 4.32DD875 pKa = 3.29AVLTVDD881 pKa = 3.6AAHH884 pKa = 6.82GVLANDD890 pKa = 3.57NDD892 pKa = 4.23ANSDD896 pKa = 3.42ALTAVLGQGPTYY908 pKa = 9.43GTLVLNADD916 pKa = 3.44GSFIYY921 pKa = 9.37TANANYY927 pKa = 10.46NGADD931 pKa = 3.16AFTYY935 pKa = 7.95TAHH938 pKa = 7.23DD939 pKa = 3.93AYY941 pKa = 10.55GGVSGVVTVQLGVEE955 pKa = 4.34AVNDD959 pKa = 4.22APTGAADD966 pKa = 3.57AASVAEE972 pKa = 4.38DD973 pKa = 3.36ASILVNVLANDD984 pKa = 3.75HH985 pKa = 7.16DD986 pKa = 4.38VDD988 pKa = 5.83LDD990 pKa = 3.87GLSIAALSGTKK1001 pKa = 9.71SALGASISIEE1011 pKa = 3.96NGQVRR1016 pKa = 11.84YY1017 pKa = 9.26AADD1020 pKa = 3.67ADD1022 pKa = 4.44GFDD1025 pKa = 3.85QLAAGHH1031 pKa = 5.83SVVDD1035 pKa = 3.82SFTYY1039 pKa = 9.72TATDD1043 pKa = 3.09GHH1045 pKa = 6.97GGFTAPITVSITVGEE1060 pKa = 4.35AGDD1063 pKa = 3.77NRR1065 pKa = 11.84TLSGNPVKK1073 pKa = 10.81ANSFTDD1079 pKa = 3.12VGEE1082 pKa = 4.12YY1083 pKa = 8.48DD1084 pKa = 3.26TTYY1087 pKa = 10.72TGGLLSDD1094 pKa = 4.6VINGGGGSDD1103 pKa = 3.84HH1104 pKa = 7.14LFGGNGKK1111 pKa = 8.45DD1112 pKa = 3.46TLNGGTGRR1120 pKa = 11.84DD1121 pKa = 3.58TLEE1124 pKa = 4.75GGTGPDD1130 pKa = 4.33LLTGGAGADD1139 pKa = 3.34DD1140 pKa = 4.24FVFGLGSGTDD1150 pKa = 3.82HH1151 pKa = 6.28ITDD1154 pKa = 4.65FNPGEE1159 pKa = 4.06DD1160 pKa = 4.4HH1161 pKa = 7.38IITPLRR1167 pKa = 11.84DD1168 pKa = 3.35QLGDD1172 pKa = 3.55FVGGLGLGKK1181 pKa = 10.28LADD1184 pKa = 3.82LVDD1187 pKa = 4.01RR1188 pKa = 11.84LLAPSFKK1195 pKa = 10.9DD1196 pKa = 3.0VDD1198 pKa = 4.15TNGDD1202 pKa = 3.11HH1203 pKa = 7.0HH1204 pKa = 7.9ADD1206 pKa = 3.18AVSINAVGMGTVVLDD1221 pKa = 3.19NWTIATLTAQGYY1233 pKa = 10.29LDD1235 pKa = 4.26PQHH1238 pKa = 6.76HH1239 pKa = 6.6VVGDD1243 pKa = 3.58WLLL1246 pKa = 3.58

Molecular weight: 126.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q7TIU5|A0A0Q7TIU5_9CAUL Chemotaxis protein OS=Caulobacter sp. Root1455 OX=1736465 GN=ASD21_22400 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 9.58LVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 5.26GYY18 pKa = 7.96RR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 9.84NGQKK29 pKa = 9.53IVARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.77GRR39 pKa = 11.84KK40 pKa = 9.04RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4531

0

4531

1466033

29

4887

323.6

34.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.936 ± 0.057

0.735 ± 0.012

5.98 ± 0.029

5.148 ± 0.038

3.578 ± 0.023

9.131 ± 0.07

1.817 ± 0.017

4.273 ± 0.021

3.36 ± 0.029

10.009 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.16 ± 0.019

2.393 ± 0.028

5.618 ± 0.035

3.094 ± 0.023

6.936 ± 0.046

5.046 ± 0.034

5.409 ± 0.04

7.683 ± 0.029

1.471 ± 0.016

2.222 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski