Lacimicrobium alkaliphilum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae; Lacimicrobium

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3754 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U3ALQ6|A0A0U3ALQ6_9ALTE Uncharacterized protein OS=Lacimicrobium alkaliphilum OX=1526571 GN=AT746_11950 PE=4 SV=1
MM1 pKa = 7.07QVTAAPADD9 pKa = 3.59SLIISGVVDD18 pKa = 4.55GPLPGGLPKK27 pKa = 10.48AVEE30 pKa = 4.3VYY32 pKa = 9.45VAKK35 pKa = 10.83DD36 pKa = 2.86IDD38 pKa = 5.23DD39 pKa = 3.96ISQCGLGSANNGGGTDD55 pKa = 3.7GEE57 pKa = 4.61EE58 pKa = 3.97FTFPSGAVSAGQYY71 pKa = 10.36LYY73 pKa = 10.9VASEE77 pKa = 4.33SAGFTDD83 pKa = 5.92FFGFSPNYY91 pKa = 8.66TSSAANINGDD101 pKa = 3.61DD102 pKa = 4.74AIEE105 pKa = 4.18LFCDD109 pKa = 3.97GAVVDD114 pKa = 4.45TFGEE118 pKa = 4.51TEE120 pKa = 3.87VDD122 pKa = 3.12GSGQVWEE129 pKa = 4.79YY130 pKa = 11.14LDD132 pKa = 3.19GWAYY136 pKa = 10.13RR137 pKa = 11.84VAEE140 pKa = 4.31TGPDD144 pKa = 2.97AGNFVQANWQFSGPNALDD162 pKa = 3.75NEE164 pKa = 4.58SSNASAAVPFPLATFAGDD182 pKa = 3.84GGDD185 pKa = 3.81TPVDD189 pKa = 4.77PIDD192 pKa = 5.29PDD194 pKa = 5.17PIDD197 pKa = 4.93PDD199 pKa = 3.61PVEE202 pKa = 4.45PGEE205 pKa = 4.39GGEE208 pKa = 4.08GLIFSEE214 pKa = 4.6YY215 pKa = 10.95VEE217 pKa = 4.23GSSFNKK223 pKa = 10.25ALEE226 pKa = 4.27LANTTDD232 pKa = 3.07STLDD236 pKa = 3.3LGGYY240 pKa = 8.34QVLLYY245 pKa = 10.95SNSNTTAGTTLDD257 pKa = 3.88LSGSLAAGEE266 pKa = 4.3VLVIANSQAGDD277 pKa = 4.67AIQSVADD284 pKa = 3.46IVSGVTNFNGNDD296 pKa = 3.65ALEE299 pKa = 4.08LRR301 pKa = 11.84YY302 pKa = 9.99NDD304 pKa = 4.32EE305 pKa = 4.51LVDD308 pKa = 4.41SIGQVGSGQYY318 pKa = 10.03FGSNVTLVRR327 pKa = 11.84KK328 pKa = 9.92ADD330 pKa = 3.35ITAGDD335 pKa = 4.1TDD337 pKa = 3.91SSDD340 pKa = 3.42TFVAADD346 pKa = 3.27QWQEE350 pKa = 3.81FASDD354 pKa = 3.16TFTYY358 pKa = 10.33LGSHH362 pKa = 6.39NGDD365 pKa = 4.09DD366 pKa = 4.77GGDD369 pKa = 3.55DD370 pKa = 3.79TPVVEE375 pKa = 5.86LGQCGDD381 pKa = 3.7PATLISQVQGSGDD394 pKa = 3.47VSPVVSEE401 pKa = 3.77SHH403 pKa = 6.85IIEE406 pKa = 4.24AVVTGTFTNLQGYY419 pKa = 7.49FVQEE423 pKa = 4.43EE424 pKa = 4.26VADD427 pKa = 3.94WDD429 pKa = 3.94NDD431 pKa = 3.16ASTSEE436 pKa = 4.19GVFVYY441 pKa = 10.36TNSDD445 pKa = 3.14QNLPQPGTLVRR456 pKa = 11.84VQGEE460 pKa = 3.99VSEE463 pKa = 5.47FFDD466 pKa = 3.8KK467 pKa = 10.66TQLTATASALDD478 pKa = 4.15CGQADD483 pKa = 3.99TATVSLSLPFADD495 pKa = 4.0AQQTEE500 pKa = 4.61TLEE503 pKa = 4.2GMLVSFDD510 pKa = 3.84HH511 pKa = 6.67SLTVTDD517 pKa = 4.28NYY519 pKa = 11.43NLGRR523 pKa = 11.84YY524 pKa = 9.76GEE526 pKa = 4.41VTLSNGRR533 pKa = 11.84TYY535 pKa = 10.82IPTNLHH541 pKa = 6.25LPGSPEE547 pKa = 3.74AQALAEE553 pKa = 4.02QNALNRR559 pKa = 11.84ILLDD563 pKa = 4.75DD564 pKa = 5.7GINGQNPATVIYY576 pKa = 7.3PTGGLSAGNTLRR588 pKa = 11.84TGDD591 pKa = 4.03EE592 pKa = 4.17VSALTGVLDD601 pKa = 3.9YY602 pKa = 11.71GFGEE606 pKa = 4.17YY607 pKa = 10.07RR608 pKa = 11.84IIPVEE613 pKa = 4.04APTFVASNPRR623 pKa = 11.84TPAPEE628 pKa = 3.71IAEE631 pKa = 4.46GNLKK635 pKa = 10.11VASLNVLNFFNGNGLGGGFPTPRR658 pKa = 11.84GADD661 pKa = 3.04NVEE664 pKa = 4.17EE665 pKa = 4.25YY666 pKa = 10.44QRR668 pKa = 11.84QLDD671 pKa = 4.0KK672 pKa = 11.21LVSAILAMDD681 pKa = 4.56ADD683 pKa = 3.97IVGLMEE689 pKa = 5.03IEE691 pKa = 4.07NDD693 pKa = 4.49GYY695 pKa = 11.26DD696 pKa = 3.51QNSAIAQLTDD706 pKa = 3.47ALNAQAGDD714 pKa = 3.4AVYY717 pKa = 10.45QYY719 pKa = 11.4VNAGGPLGTDD729 pKa = 4.24AIAVGLLYY737 pKa = 10.49KK738 pKa = 10.73ANTVTPVGDD747 pKa = 3.56VRR749 pKa = 11.84IHH751 pKa = 7.01LDD753 pKa = 3.26EE754 pKa = 4.71VYY756 pKa = 10.75NRR758 pKa = 11.84PPLAQRR764 pKa = 11.84FSNTEE769 pKa = 3.68GGEE772 pKa = 3.74LSVIVNHH779 pKa = 6.41FKK781 pKa = 11.2SKK783 pKa = 10.38GGCGSAEE790 pKa = 4.92GLDD793 pKa = 3.62QDD795 pKa = 4.66QGDD798 pKa = 4.05GQACFNATRR807 pKa = 11.84VAQAQALLDD816 pKa = 4.26WIATDD821 pKa = 3.63EE822 pKa = 4.1QLSGEE827 pKa = 4.26EE828 pKa = 3.77NHH830 pKa = 7.1LVIGDD835 pKa = 4.0LNAYY839 pKa = 9.88AKK841 pKa = 9.82EE842 pKa = 3.98DD843 pKa = 4.51PIRR846 pKa = 11.84EE847 pKa = 4.17FTDD850 pKa = 2.97NGYY853 pKa = 8.57TNLIEE858 pKa = 5.09AVAGPQAYY866 pKa = 9.68SYY868 pKa = 11.32VFAGEE873 pKa = 4.03AGYY876 pKa = 10.58LDD878 pKa = 3.93HH879 pKa = 7.55AIASDD884 pKa = 3.42ALAAQVVDD892 pKa = 3.54VTEE895 pKa = 3.66WHH897 pKa = 6.81INADD901 pKa = 3.6EE902 pKa = 4.31PRR904 pKa = 11.84ILDD907 pKa = 3.51YY908 pKa = 10.31NTEE911 pKa = 4.27FKK913 pKa = 10.89SQQQVLDD920 pKa = 4.98FYY922 pKa = 11.79APDD925 pKa = 3.44SYY927 pKa = 10.81RR928 pKa = 11.84ASDD931 pKa = 4.01HH932 pKa = 7.18DD933 pKa = 4.17PVIVTLDD940 pKa = 3.43LQAAEE945 pKa = 4.37LQGDD949 pKa = 4.06FDD951 pKa = 5.49GDD953 pKa = 3.81GDD955 pKa = 4.21VDD957 pKa = 4.19SSDD960 pKa = 3.35MKK962 pKa = 11.37GLMRR966 pKa = 11.84AILTRR971 pKa = 11.84QSIDD975 pKa = 3.01MAFDD979 pKa = 4.22LNGDD983 pKa = 3.75GVINTRR989 pKa = 11.84DD990 pKa = 3.59LQILFTLCTRR1000 pKa = 11.84ANCATEE1006 pKa = 3.7

Molecular weight: 105.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U3B2A8|A0A0U3B2A8_9ALTE HTH araC/xylS-type domain-containing protein OS=Lacimicrobium alkaliphilum OX=1526571 GN=AT746_14710 PE=4 SV=1
MM1 pKa = 7.15QFIAKK6 pKa = 8.73IQSARR11 pKa = 11.84SFRR14 pKa = 11.84NCNTALLRR22 pKa = 11.84ALCDD26 pKa = 3.45SAVRR30 pKa = 11.84NAFFSGLSLSYY41 pKa = 11.12ALRR44 pKa = 11.84AAIKK48 pKa = 10.36RR49 pKa = 11.84LALKK53 pKa = 9.89IAPGDD58 pKa = 3.65FFVSFVVKK66 pKa = 9.99KK67 pKa = 9.46HH68 pKa = 4.53WANILGSPINWTLHH82 pKa = 7.08DD83 pKa = 4.01IALLPALCAPAPLRR97 pKa = 11.84EE98 pKa = 4.29PKK100 pKa = 10.7LSFSDD105 pKa = 3.49LAEE108 pKa = 4.05SLRR111 pKa = 11.84RR112 pKa = 11.84RR113 pKa = 11.84AGKK116 pKa = 9.96

Molecular weight: 12.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3754

0

3754

1254132

34

3323

334.1

37.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.018 ± 0.043

1.011 ± 0.014

5.724 ± 0.034

6.111 ± 0.039

3.988 ± 0.026

7.138 ± 0.039

2.283 ± 0.023

5.662 ± 0.028

4.508 ± 0.036

11.002 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.562 ± 0.018

3.805 ± 0.023

4.202 ± 0.025

5.452 ± 0.036

5.221 ± 0.025

6.414 ± 0.031

4.867 ± 0.027

6.556 ± 0.032

1.389 ± 0.018

3.087 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski