Devosia enhydra
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3974 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1K2I1R1|A0A1K2I1R1_9RHIZ Uncharacterized conserved protein DUF952 family OS=Devosia enhydra OX=665118 GN=SAMN02983003_3443 PE=4 SV=1
MM1 pKa = 7.79 RR2 pKa = 11.84 PHH4 pKa = 7.2 ALLLGMNAALLLSTAVSAADD24 pKa = 4.73 AITPAPVMPATVVFEE39 pKa = 4.27 EE40 pKa = 4.36 PALFSLTIEE49 pKa = 4.59 GGVSAFGMPSTDD61 pKa = 3.42 TIGTTNIGGLNGDD74 pKa = 3.54 EE75 pKa = 4.26 VLFEE79 pKa = 4.84 GLDD82 pKa = 3.55 DD83 pKa = 4.04 MLFGGNLAAKK93 pKa = 9.87 FSAPINDD100 pKa = 3.43 SGAGVTVSGFGAFATRR116 pKa = 11.84 SGSQSRR122 pKa = 11.84 VLDD125 pKa = 3.81 DD126 pKa = 3.68 EE127 pKa = 4.53 RR128 pKa = 11.84 LLVIHH133 pKa = 7.06 GPTLPVGTIGMYY145 pKa = 8.81 TWDD148 pKa = 4.47 LDD150 pKa = 4.44 GIATGTGVGDD160 pKa = 3.96 LGDD163 pKa = 4.28 AEE165 pKa = 4.58 STVTAGGGVAIPTANAATILGPVQDD190 pKa = 4.67 VEE192 pKa = 4.39 SSPDD196 pKa = 3.0 GFTYY200 pKa = 10.62 AGVIGDD206 pKa = 4.01 YY207 pKa = 9.04 QTFSLGALATTDD219 pKa = 2.93 GAMFVGYY226 pKa = 10.7 GEE228 pKa = 4.15 LLGWEE233 pKa = 4.33 VSTDD237 pKa = 3.0 VTQNMVHH244 pKa = 6.46 SGADD248 pKa = 3.3 LTFSQTSSPDD258 pKa = 3.15 GGNVAFTGYY267 pKa = 10.44 AGPSFRR273 pKa = 11.84 YY274 pKa = 9.93 LSTEE278 pKa = 3.81 TTTQTSVTPRR288 pKa = 11.84 QFAEE292 pKa = 4.0 YY293 pKa = 10.5 APDD296 pKa = 3.42 FEE298 pKa = 4.84 YY299 pKa = 11.52 AMFSLDD305 pKa = 3.75 TIEE308 pKa = 5.63 TIDD311 pKa = 3.25 TYY313 pKa = 11.33 YY314 pKa = 11.2 AGGVVGGNIDD324 pKa = 3.21 VSLWSGSTLSLGVGGGAYY342 pKa = 9.71 YY343 pKa = 10.79 ASSHH347 pKa = 5.52 YY348 pKa = 10.88 ASEE351 pKa = 4.53 TEE353 pKa = 3.92 ATLSGGSAAADD364 pKa = 2.97 EE365 pKa = 5.28 SVFSTLNLEE374 pKa = 4.45 PEE376 pKa = 3.96 TGFAYY381 pKa = 8.53 TAKK384 pKa = 10.74 ADD386 pKa = 4.01 GKK388 pKa = 9.7 FSAHH392 pKa = 5.83 IGEE395 pKa = 4.2 RR396 pKa = 11.84 LQLNFGLGAEE406 pKa = 4.21 YY407 pKa = 10.61 LSRR410 pKa = 11.84 VASARR415 pKa = 11.84 FAGSNGMTVSDD426 pKa = 3.82 TAGDD430 pKa = 3.91 DD431 pKa = 3.62 VGVPDD436 pKa = 5.77 ADD438 pKa = 3.58 FDD440 pKa = 4.56 SSNATGSTILTFGDD454 pKa = 3.1 AMSYY458 pKa = 10.35 KK459 pKa = 9.88 ATIGLTGQFF468 pKa = 3.77
Molecular weight: 47.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.698
IPC2_protein 3.745
IPC_protein 3.77
Toseland 3.554
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.694
Rodwell 3.592
Grimsley 3.452
Solomon 3.757
Lehninger 3.706
Nozaki 3.872
DTASelect 4.113
Thurlkill 3.605
EMBOSS 3.706
Sillero 3.897
Patrickios 0.973
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.774
Protein with the highest isoelectric point:
>tr|A0A1K2I211|A0A1K2I211_9RHIZ Uncharacterized protein OS=Devosia enhydra OX=665118 GN=SAMN02983003_3602 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.74 LVRR12 pKa = 11.84 TRR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.65 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 NGRR28 pKa = 11.84 KK29 pKa = 9.04 ILNLRR34 pKa = 11.84 RR35 pKa = 11.84 AVGRR39 pKa = 11.84 KK40 pKa = 9.1 KK41 pKa = 10.71 LSAA44 pKa = 3.93
Molecular weight: 5.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.413
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.034
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3974
0
3974
1260071
24
2366
317.1
34.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.106 ± 0.052
0.701 ± 0.01
5.605 ± 0.029
5.587 ± 0.032
3.667 ± 0.026
8.875 ± 0.042
1.951 ± 0.019
5.314 ± 0.026
2.568 ± 0.031
10.489 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.504 ± 0.02
2.392 ± 0.025
5.469 ± 0.033
2.976 ± 0.021
7.267 ± 0.04
5.313 ± 0.028
5.31 ± 0.028
7.471 ± 0.033
1.3 ± 0.018
2.134 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here