Clostridium grantii DSM 8605

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; Clostridium grantii

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4177 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M5V8C2|A0A1M5V8C2_9CLOT Alpha/beta hydrolase family protein OS=Clostridium grantii DSM 8605 OX=1121316 GN=SAMN02745207_02166 PE=4 SV=1
MM1 pKa = 7.79ANTFTLTMTSIALDD15 pKa = 4.45AISIEE20 pKa = 4.32LGSTASTTLIDD31 pKa = 3.86SMIKK35 pKa = 9.22IRR37 pKa = 11.84KK38 pKa = 8.67FNGSTFDD45 pKa = 4.5DD46 pKa = 4.01YY47 pKa = 11.96TDD49 pKa = 3.95FTVSGASDD57 pKa = 3.6TVATINPNTDD67 pKa = 3.78FSQTDD72 pKa = 3.53MILIQIQDD80 pKa = 3.46GTDD83 pKa = 3.16YY84 pKa = 11.24SDD86 pKa = 3.47RR87 pKa = 11.84VLVDD91 pKa = 3.77FAQDD95 pKa = 3.39YY96 pKa = 11.69DD97 pKa = 4.29EE98 pKa = 5.03TNHH101 pKa = 6.84DD102 pKa = 3.51ADD104 pKa = 4.06YY105 pKa = 10.69KK106 pKa = 11.15YY107 pKa = 10.47EE108 pKa = 4.36LPAQTGSYY116 pKa = 9.79KK117 pKa = 10.26IQLDD121 pKa = 4.16TVNGTDD127 pKa = 3.7FSTVEE132 pKa = 3.91SAAFAVGNAPTGATVSIAKK151 pKa = 10.1DD152 pKa = 3.49YY153 pKa = 10.34LVKK156 pKa = 10.49YY157 pKa = 7.98TGQVLDD163 pKa = 4.6VIVTLTGTDD172 pKa = 3.22IPAGNYY178 pKa = 7.46EE179 pKa = 3.97VSIDD183 pKa = 4.75ADD185 pKa = 3.8LQQ187 pKa = 3.73

Molecular weight: 20.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M5U4U2|A0A1M5U4U2_9CLOT Alanine racemase OS=Clostridium grantii DSM 8605 OX=1121316 GN=SAMN02745207_01574 PE=3 SV=1
MM1 pKa = 7.34ARR3 pKa = 11.84KK4 pKa = 9.87AMLEE8 pKa = 4.06KK9 pKa = 9.92WSKK12 pKa = 7.28TPKK15 pKa = 8.65YY16 pKa = 7.33QTRR19 pKa = 11.84AYY21 pKa = 7.9TRR23 pKa = 11.84CRR25 pKa = 11.84ICGRR29 pKa = 11.84PHH31 pKa = 6.08SVLRR35 pKa = 11.84KK36 pKa = 9.51YY37 pKa = 9.91GICRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 3.97LAYY49 pKa = 9.88KK50 pKa = 10.17GQIPGCKK57 pKa = 8.74KK58 pKa = 10.74ASWW61 pKa = 3.34

Molecular weight: 7.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4177

0

4177

1287820

39

3273

308.3

34.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.45 ± 0.039

1.136 ± 0.014

5.446 ± 0.034

7.729 ± 0.043

4.632 ± 0.033

6.179 ± 0.041

1.383 ± 0.015

10.067 ± 0.052

8.973 ± 0.036

9.049 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.668 ± 0.02

6.378 ± 0.039

2.704 ± 0.02

2.398 ± 0.018

3.077 ± 0.023

6.416 ± 0.024

4.983 ± 0.034

6.301 ± 0.03

0.777 ± 0.013

4.253 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski