Chickpea yellow dwarf virus
Average proteome isoelectric point is 7.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A0UF55|A0A0A0UF55_9GEMI Replication-associated protein OS=Chickpea yellow dwarf virus OX=1568974 PE=3 SV=1
MM1 pKa = 7.96 PRR3 pKa = 11.84 RR4 pKa = 11.84 TNNNSFRR11 pKa = 11.84 LQTKK15 pKa = 9.03 YY16 pKa = 11.1 VFLTYY21 pKa = 9.65 PHH23 pKa = 6.74 SSSTAEE29 pKa = 3.99 NLRR32 pKa = 11.84 DD33 pKa = 3.88 FLWSKK38 pKa = 9.02 LTSLAIFFIAIATEE52 pKa = 4.05 LHH54 pKa = 6.12 QDD56 pKa = 3.96 GSPHH60 pKa = 5.94 LHH62 pKa = 6.98 CLIQLDD68 pKa = 4.15 KK69 pKa = 11.07 RR70 pKa = 11.84 CDD72 pKa = 3.2 IRR74 pKa = 11.84 DD75 pKa = 3.3 PSFFDD80 pKa = 4.7 FEE82 pKa = 4.9 GNHH85 pKa = 6.78 PNIQPAKK92 pKa = 9.43 NSKK95 pKa = 9.78 QVLEE99 pKa = 4.68 YY100 pKa = 10.19 ISKK103 pKa = 10.52 DD104 pKa = 3.12 GNIITRR110 pKa = 11.84 GDD112 pKa = 3.55 FRR114 pKa = 11.84 NHH116 pKa = 5.79 RR117 pKa = 11.84 VSPTKK122 pKa = 10.37 SDD124 pKa = 3.41 EE125 pKa = 3.8 RR126 pKa = 11.84 WRR128 pKa = 11.84 TIIQTATSKK137 pKa = 11.14 EE138 pKa = 4.22 EE139 pKa = 3.73 YY140 pKa = 10.77 LEE142 pKa = 4.31 MIKK145 pKa = 10.12 QEE147 pKa = 4.85 FPHH150 pKa = 6.17 EE151 pKa = 4.21 WATKK155 pKa = 8.46 LHH157 pKa = 5.33 WLEE160 pKa = 4.13 YY161 pKa = 9.8 SASKK165 pKa = 10.35 LFPDD169 pKa = 4.83 IEE171 pKa = 4.43 PPYY174 pKa = 10.38 QSPFPDD180 pKa = 3.39 EE181 pKa = 4.76 FLHH184 pKa = 5.57 CHH186 pKa = 5.47 EE187 pKa = 6.07 EE188 pKa = 3.66 ITEE191 pKa = 3.97 WLNRR195 pKa = 11.84 DD196 pKa = 3.89 LYY198 pKa = 10.6 VVSVDD203 pKa = 4.1 SYY205 pKa = 11.68 QLLHH209 pKa = 6.46 PHH211 pKa = 7.01 LSLEE215 pKa = 4.38 TATEE219 pKa = 3.91 DD220 pKa = 5.1 LIWMDD225 pKa = 4.75 DD226 pKa = 3.6 YY227 pKa = 11.67 TRR229 pKa = 11.84 NPNNSNSGEE238 pKa = 4.14 DD239 pKa = 3.66 PSTSADD245 pKa = 3.2 QVVPEE250 pKa = 4.58 RR251 pKa = 11.84 PVGPEE256 pKa = 3.28 VWEE259 pKa = 4.31 DD260 pKa = 3.53 TTTSTEE266 pKa = 4.12 GSISLHH272 pKa = 4.7 TTSTQHH278 pKa = 5.2 TTSSTTSPSSSAPTGSSS295 pKa = 2.86
Molecular weight: 33.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.051
IPC2_protein 5.054
IPC_protein 5.029
Toseland 5.08
ProMoST 5.207
Dawson 5.08
Bjellqvist 5.181
Wikipedia 4.965
Rodwell 5.003
Grimsley 5.041
Solomon 5.08
Lehninger 5.041
Nozaki 5.219
DTASelect 5.385
Thurlkill 5.105
EMBOSS 5.067
Sillero 5.308
Patrickios 3.884
IPC_peptide 5.092
IPC2_peptide 5.308
IPC2.peptide.svr19 5.206
Protein with the highest isoelectric point:
>tr|A0A0A0U8Q7|A0A0A0U8Q7_9GEMI Replication-associated protein OS=Chickpea yellow dwarf virus OX=1568974 PE=3 SV=1
MM1 pKa = 7.93 PGPFSGKK8 pKa = 8.25 TYY10 pKa = 10.28 SRR12 pKa = 11.84 KK13 pKa = 8.86 KK14 pKa = 10.22 GKK16 pKa = 8.59 YY17 pKa = 7.81 AKK19 pKa = 9.89 AYY21 pKa = 8.61 KK22 pKa = 10.5 ALGVKK27 pKa = 7.95 NQKK30 pKa = 8.75 EE31 pKa = 4.13 LEE33 pKa = 4.19 EE34 pKa = 4.38 LVNAPACPVTPRR46 pKa = 11.84 PALQVAEE53 pKa = 4.51 YY54 pKa = 10.17 FWTTDD59 pKa = 2.93 KK60 pKa = 11.59 NGMIFRR66 pKa = 11.84 SGGGTAHH73 pKa = 6.29 FTMYY77 pKa = 10.3 PQGSNEE83 pKa = 3.93 NCRR86 pKa = 11.84 HH87 pKa = 5.44 SNQTNTYY94 pKa = 10.01 KK95 pKa = 10.12 MAIKK99 pKa = 10.04 CWVALDD105 pKa = 3.32 PTFYY109 pKa = 11.0 KK110 pKa = 10.48 KK111 pKa = 10.05 VACVPVHH118 pKa = 6.13 FWLVYY123 pKa = 10.81 DD124 pKa = 4.57 KK125 pKa = 11.45 DD126 pKa = 4.0 PGNTLPGCSTIFDD139 pKa = 4.39 TLYY142 pKa = 10.62 QDD144 pKa = 5.17 YY145 pKa = 9.93 PGTWTVSRR153 pKa = 11.84 NVSRR157 pKa = 11.84 RR158 pKa = 11.84 FVVKK162 pKa = 9.84 KK163 pKa = 8.83 HH164 pKa = 4.1 WHH166 pKa = 6.64 IILASNGTNPTQDD179 pKa = 3.16 QDD181 pKa = 3.62 PAKK184 pKa = 9.85 YY185 pKa = 9.91 AGPGPVFQWKK195 pKa = 9.76 HH196 pKa = 3.83 MNKK199 pKa = 8.99 FFKK202 pKa = 10.59 RR203 pKa = 11.84 LGVRR207 pKa = 11.84 TDD209 pKa = 3.17 WKK211 pKa = 10.78 NSATGEE217 pKa = 4.17 VADD220 pKa = 4.34 IKK222 pKa = 11.13 SGALYY227 pKa = 9.62 LVCAPSGGAVVRR239 pKa = 11.84 VGGRR243 pKa = 11.84 FRR245 pKa = 11.84 MYY247 pKa = 10.18 FKK249 pKa = 10.96 SVGNQQ254 pKa = 2.8
Molecular weight: 28.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.169
IPC2_protein 9.224
IPC_protein 9.121
Toseland 9.794
ProMoST 9.531
Dawson 10.043
Bjellqvist 9.75
Wikipedia 10.204
Rodwell 10.452
Grimsley 10.116
Solomon 10.058
Lehninger 10.028
Nozaki 9.867
DTASelect 9.721
Thurlkill 9.882
EMBOSS 10.218
Sillero 9.97
Patrickios 7.541
IPC_peptide 10.058
IPC2_peptide 8.478
IPC2.peptide.svr19 8.199
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
986
101
336
246.5
28.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.97 ± 0.849
1.724 ± 0.297
5.172 ± 0.654
5.781 ± 1.129
5.274 ± 0.353
5.375 ± 1.321
3.448 ± 0.553
5.172 ± 1.026
6.187 ± 1.015
7.099 ± 0.728
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.42 ± 0.289
5.274 ± 0.211
6.389 ± 0.345
3.753 ± 0.074
5.071 ± 0.358
8.418 ± 1.299
7.708 ± 0.878
5.274 ± 1.286
2.231 ± 0.29
4.26 ± 0.446
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here