Metschnikowia aff. pulcherrima

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Metschnikowiaceae; Metschnikowia

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5795 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P6XPW9|A0A4P6XPW9_9ASCO Post-GPI attachment to proteins factor 3 OS=Metschnikowia aff. pulcherrima OX=2163413 GN=MPUL0D06180 PE=3 SV=1
MM1 pKa = 6.97KK2 pKa = 9.96TGIGALATAFMCLFGVISAVTITQNTVEE30 pKa = 4.34VSPISLSLGEE40 pKa = 5.13LYY42 pKa = 10.46INPGVYY48 pKa = 10.4YY49 pKa = 10.51SIVNNFQTLLARR61 pKa = 11.84SVYY64 pKa = 10.62NKK66 pKa = 10.43GGLFITAADD75 pKa = 4.07SYY77 pKa = 8.8TASVTMTGDD86 pKa = 2.85TFMNSGIVSFNSLSASKK103 pKa = 10.33ISQYY107 pKa = 11.25YY108 pKa = 7.22ITTITSFLNTGSMFFGVSGATLLNTPYY135 pKa = 10.09YY136 pKa = 9.93VKK138 pKa = 10.34SVYY141 pKa = 9.94SWSNTGMMVFKK152 pKa = 9.79RR153 pKa = 11.84TSGSAGSVVIHH164 pKa = 6.5LSVDD168 pKa = 3.21SSGVPTITNAGSICLYY184 pKa = 9.06NTDD187 pKa = 3.51WTQSTSIKK195 pKa = 10.46GSGCITVGPEE205 pKa = 3.78SQFNLEE211 pKa = 3.73MTAYY215 pKa = 9.87GISAAQTFYY224 pKa = 11.22LSSADD229 pKa = 3.51SVLSIHH235 pKa = 6.27GVVPGSSAYY244 pKa = 7.56PTYY247 pKa = 10.47RR248 pKa = 11.84VAGFGGGNTININLSFSKK266 pKa = 10.59FSYY269 pKa = 10.35SATTGVLTLSSALFTVYY286 pKa = 10.21FNIGTGYY293 pKa = 10.44KK294 pKa = 10.09SSLFSTNTAMMGRR307 pKa = 11.84SITYY311 pKa = 9.42DD312 pKa = 3.4GPPPNSAPAVCSCEE326 pKa = 3.49WSFPSVTTSLIASSTTAKK344 pKa = 10.26SAANSVTSASSSSSSSPVTEE364 pKa = 3.96STNVSEE370 pKa = 4.99LSSASASRR378 pKa = 11.84STVEE382 pKa = 4.01LSSSVVSDD390 pKa = 3.19SSVISTTTTVADD402 pKa = 4.0PFDD405 pKa = 4.01FSSFFEE411 pKa = 4.56KK412 pKa = 10.49SDD414 pKa = 3.61SSLTASLASASSPSAAVDD432 pKa = 4.33FSLTSSAIVASSLSSDD448 pKa = 3.43YY449 pKa = 11.58DD450 pKa = 3.65LASISTLTSASSSSANSYY468 pKa = 11.31SSLTSSSIVASSSSDD483 pKa = 3.02AFEE486 pKa = 4.79SPLTSSLATASSSSATFEE504 pKa = 4.46SSMSPSSIVASSSSAAFEE522 pKa = 4.7SPLSSSLATASSSSDD537 pKa = 2.95AFEE540 pKa = 4.83SPLTSSANVASSLSSDD556 pKa = 3.36SGLAATSSLASASSSSAGLSTSLSPSSIVASSSSAALEE594 pKa = 4.15SSLTSSLATASSSSAAFEE612 pKa = 4.52SSLSSNLATASSSSATFEE630 pKa = 4.46SSMSPSSIVSSSSSDD645 pKa = 3.1AFEE648 pKa = 4.83SPLTSSANVASSLSSDD664 pKa = 3.36SGLAATSSLASASSSSAGLSTSLSPSSIVASSSSAALEE702 pKa = 4.15SSLTSSLATASSSSAAFEE720 pKa = 4.52SSLSSNLATASSSSATFEE738 pKa = 4.46SSMSPSSIVSSSSSDD753 pKa = 3.1AFEE756 pKa = 4.83SPLTSSANVASSLSSDD772 pKa = 3.36SGLAATSSLASASSSSAGLSTSLSPSSIVASSSSAALEE810 pKa = 4.15SSLTSSLATASSSSAAFEE828 pKa = 4.52SSLSSNLATASSSSATFEE846 pKa = 4.46SSMSPSSIVSSSSSDD861 pKa = 3.1AFEE864 pKa = 4.83SPLTSSANVASSLSSDD880 pKa = 3.36SGLAATSSLASASSSSAGLSTSLSPSSIVASSSSAALEE918 pKa = 4.15SSLTSSLATASSSSAAFEE936 pKa = 4.37SSLTSSLATASSSSATFEE954 pKa = 4.46SSMSPSSIVSSSSSAAFEE972 pKa = 4.37SSLTSSLATASSSSAAFEE990 pKa = 4.54SSLSSSLATASGSSATFEE1008 pKa = 4.58SSMSPSSIVSSSSSAAFEE1026 pKa = 4.37SSLTSSLATASSSSAAFEE1044 pKa = 4.54SSLSSSLATASGSSATFEE1062 pKa = 4.58SSMSPSSIVASSSSAAFEE1080 pKa = 4.7SPLSSSLATASSSSDD1095 pKa = 2.95AFEE1098 pKa = 4.83SPLTSSANVASSLSSDD1114 pKa = 3.36SGLAATSSLASASSSSAGLSTSLSPSSIVASSSSADD1150 pKa = 3.21SSMLSAFDD1158 pKa = 3.77SSVTRR1163 pKa = 11.84DD1164 pKa = 3.59LTSSSSLAGLSLSSTSTSPVDD1185 pKa = 3.84FNSFITSSSGSTSGLSFAQKK1205 pKa = 9.05TSSGAISSLAMGSEE1219 pKa = 4.31SVQRR1223 pKa = 11.84FTTTLVSSKK1232 pKa = 10.73SGDD1235 pKa = 3.48SSASEE1240 pKa = 3.81PDD1242 pKa = 3.23AASVTRR1248 pKa = 11.84SLSSFVSGKK1257 pKa = 10.44SLDD1260 pKa = 3.75EE1261 pKa = 4.07NAISSSPGSVTDD1273 pKa = 4.04LPVISSSVFLGYY1285 pKa = 10.56HH1286 pKa = 5.62NSSVGMSSSSWNSMSSLAQGTKK1308 pKa = 9.1STVTSRR1314 pKa = 11.84TNSGGLSSTVSTGSMSADD1332 pKa = 2.97LVTASDD1338 pKa = 4.43FSLSATEE1345 pKa = 4.19TTSAGASASATQPGAGGTSAPLSSRR1370 pKa = 11.84TDD1372 pKa = 3.88FPVSSSSILGVGPSVVSISGHH1393 pKa = 4.8SAASIDD1399 pKa = 3.89VSATAAYY1406 pKa = 8.09PSTKK1410 pKa = 9.69ASSVIASGSSVVFGFASASGKK1431 pKa = 6.71TTASSNQFGSSALIEE1446 pKa = 4.42SATDD1450 pKa = 3.33AKK1452 pKa = 11.09NSIVLEE1458 pKa = 4.42TPALVNTATVTSEE1471 pKa = 3.56FDD1473 pKa = 2.92HH1474 pKa = 6.54YY1475 pKa = 11.52GAVSSDD1481 pKa = 3.27GASTSGGDD1489 pKa = 3.43VVVNKK1494 pKa = 9.86PADD1497 pKa = 4.09DD1498 pKa = 3.97YY1499 pKa = 11.84EE1500 pKa = 4.36EE1501 pKa = 5.09ASLTVSAISTDD1512 pKa = 3.46DD1513 pKa = 3.39VAVAEE1518 pKa = 4.46TQAPSTVHH1526 pKa = 6.63FSSQATRR1533 pKa = 11.84QNTWVSVSTGSSEE1546 pKa = 4.45SLSTASINIEE1556 pKa = 3.96NSAGSYY1562 pKa = 10.03NLSLAMVVLSVLALAFF1578 pKa = 4.14

Molecular weight: 154.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P6XNB3|A0A4P6XNB3_9ASCO Ribosome biogenesis protein MAK21 OS=Metschnikowia aff. pulcherrima OX=2163413 GN=MPUL0C07910 PE=3 SV=1
MM1 pKa = 6.93PTEE4 pKa = 4.21TKK6 pKa = 10.03SGKK9 pKa = 9.84RR10 pKa = 11.84NPKK13 pKa = 9.05RR14 pKa = 11.84SLWRR18 pKa = 11.84NVRR21 pKa = 11.84CRR23 pKa = 11.84IAQSPYY29 pKa = 7.91IQQNKK34 pKa = 8.6SVSRR38 pKa = 11.84EE39 pKa = 3.43MRR41 pKa = 11.84RR42 pKa = 11.84FQRR45 pKa = 11.84EE46 pKa = 3.96NQDD49 pKa = 3.15KK50 pKa = 9.08RR51 pKa = 11.84TKK53 pKa = 9.95PQIACISRR61 pKa = 11.84YY62 pKa = 9.37RR63 pKa = 11.84QLKK66 pKa = 9.04PSTPYY71 pKa = 11.12GLVRR75 pKa = 11.84CLPIVFPNIPRR86 pKa = 11.84KK87 pKa = 9.41RR88 pKa = 11.84ARR90 pKa = 11.84ALATLGDD97 pKa = 3.88FRR99 pKa = 11.84IASPARR105 pKa = 11.84CHH107 pKa = 7.62LIFCCDD113 pKa = 3.94DD114 pKa = 3.44EE115 pKa = 5.03SAVIPGRR122 pKa = 11.84ARR124 pKa = 11.84TDD126 pKa = 3.21HH127 pKa = 6.4LCAYY131 pKa = 8.75QHH133 pKa = 6.23FAHH136 pKa = 6.86GNHH139 pKa = 5.54MHH141 pKa = 6.53TLHH144 pKa = 6.87SITYY148 pKa = 7.75TLL150 pKa = 3.84

Molecular weight: 17.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5795

0

5795

2902063

50

4996

500.8

56.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.357 ± 0.03

1.215 ± 0.012

5.698 ± 0.025

6.612 ± 0.032

4.457 ± 0.021

5.533 ± 0.028

2.407 ± 0.017

5.325 ± 0.025

6.328 ± 0.03

10.368 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.102 ± 0.013

4.594 ± 0.019

4.697 ± 0.029

3.868 ± 0.023

4.861 ± 0.025

8.365 ± 0.064

5.702 ± 0.057

6.349 ± 0.025

1.044 ± 0.011

3.12 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski