Gordonia phage NadineRae

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 92 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5P8DHL4|A0A5P8DHL4_9CAUD Uncharacterized protein OS=Gordonia phage NadineRae OX=2652882 GN=46 PE=4 SV=1
MM1 pKa = 7.5TWTQPEE7 pKa = 4.43LGDD10 pKa = 4.29LVAEE14 pKa = 4.3AAGLYY19 pKa = 10.63DD20 pKa = 4.39GFNSDD25 pKa = 3.7FPEE28 pKa = 4.58GAVYY32 pKa = 10.23PDD34 pKa = 3.6IEE36 pKa = 4.31KK37 pKa = 11.02LLAKK41 pKa = 9.79VLRR44 pKa = 11.84PLVEE48 pKa = 4.36KK49 pKa = 10.49DD50 pKa = 3.11SHH52 pKa = 7.33IGNFVVSDD60 pKa = 3.83YY61 pKa = 11.31DD62 pKa = 3.32QDD64 pKa = 5.62SIDD67 pKa = 3.84EE68 pKa = 4.24DD69 pKa = 3.66TGEE72 pKa = 4.16EE73 pKa = 4.11VAGPFIEE80 pKa = 3.71IHH82 pKa = 5.85RR83 pKa = 11.84RR84 pKa = 11.84GGEE87 pKa = 3.81YY88 pKa = 10.75DD89 pKa = 3.86PDD91 pKa = 3.99DD92 pKa = 3.9FSYY95 pKa = 10.78YY96 pKa = 10.12PNVEE100 pKa = 3.78VLFWGKK106 pKa = 9.89SRR108 pKa = 11.84PVANDD113 pKa = 3.28TAHH116 pKa = 7.3KK117 pKa = 9.63GTILLLGCSGAVIDD131 pKa = 5.7GVQIDD136 pKa = 4.62LLEE139 pKa = 4.76DD140 pKa = 3.22ATGDD144 pKa = 3.66EE145 pKa = 4.84EE146 pKa = 4.93VRR148 pKa = 11.84QNNFDD153 pKa = 3.81DD154 pKa = 4.14RR155 pKa = 11.84CVTRR159 pKa = 11.84QFRR162 pKa = 11.84VGYY165 pKa = 9.99RR166 pKa = 11.84PTYY169 pKa = 10.09PDD171 pKa = 2.9

Molecular weight: 19.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5P8DFD7|A0A5P8DFD7_9CAUD Uncharacterized protein OS=Gordonia phage NadineRae OX=2652882 GN=20 PE=4 SV=1
MM1 pKa = 6.97AQRR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84GGALNTRR14 pKa = 11.84KK15 pKa = 9.95KK16 pKa = 10.91GKK18 pKa = 10.05AKK20 pKa = 10.66NGAKK24 pKa = 9.07WGHH27 pKa = 5.7GFVPKK32 pKa = 10.29NAAARR37 pKa = 11.84KK38 pKa = 9.03LKK40 pKa = 10.66KK41 pKa = 10.41KK42 pKa = 10.3LDD44 pKa = 3.9RR45 pKa = 11.84NGKK48 pKa = 8.16RR49 pKa = 11.84RR50 pKa = 11.84KK51 pKa = 8.66RR52 pKa = 11.84TAKK55 pKa = 10.28GYY57 pKa = 8.59PKK59 pKa = 10.43RR60 pKa = 4.01

Molecular weight: 6.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

92

0

92

20545

39

1801

223.3

24.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.402 ± 0.418

0.896 ± 0.119

7.209 ± 0.311

6.513 ± 0.249

3.047 ± 0.142

8.615 ± 0.384

2.044 ± 0.164

4.663 ± 0.212

4.093 ± 0.236

7.501 ± 0.26

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.229 ± 0.111

3.261 ± 0.189

5.529 ± 0.191

3.178 ± 0.15

7.462 ± 0.454

5.413 ± 0.197

6.318 ± 0.19

7.131 ± 0.194

1.918 ± 0.128

2.58 ± 0.186
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski