Broad bean mottle virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Bromoviridae; Bromovirus

Average proteome isoelectric point is 7.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q00020|1A_BBMV Replication protein 1a OS=Broad bean mottle virus OX=12301 GN=ORF1a PE=3 SV=1
MM1 pKa = 7.42SKK3 pKa = 10.08FVAADD8 pKa = 3.75EE9 pKa = 4.78YY10 pKa = 10.74IVPSFQWLLGPTSSQVEE27 pKa = 4.42SFDD30 pKa = 3.75SSVAGWIKK38 pKa = 10.35RR39 pKa = 11.84YY40 pKa = 8.89EE41 pKa = 4.38DD42 pKa = 3.49EE43 pKa = 4.45SKK45 pKa = 10.83RR46 pKa = 11.84PEE48 pKa = 4.01STVDD52 pKa = 3.66GSCEE56 pKa = 4.15SFVLAVKK63 pKa = 10.03PVMIGGQCEE72 pKa = 3.82PAYY75 pKa = 10.53DD76 pKa = 3.61QAKK79 pKa = 8.55WAEE82 pKa = 4.05TCLNVTNLASGLTGVRR98 pKa = 11.84LIPLPEE104 pKa = 4.23MARR107 pKa = 11.84MIYY110 pKa = 10.37LDD112 pKa = 4.29EE113 pKa = 4.82EE114 pKa = 4.61DD115 pKa = 4.31SFVDD119 pKa = 4.07EE120 pKa = 5.42SEE122 pKa = 4.09VDD124 pKa = 2.9DD125 pKa = 5.08WYY127 pKa = 10.55PEE129 pKa = 3.95DD130 pKa = 3.63TSDD133 pKa = 3.86GFEE136 pKa = 4.13YY137 pKa = 10.85LSADD141 pKa = 3.38GSDD144 pKa = 3.33YY145 pKa = 10.91HH146 pKa = 7.32QNSEE150 pKa = 4.25PEE152 pKa = 4.13EE153 pKa = 4.06EE154 pKa = 4.83VLDD157 pKa = 4.08KK158 pKa = 11.77SNGTLEE164 pKa = 4.26SEE166 pKa = 4.75EE167 pKa = 4.42ARR169 pKa = 11.84HH170 pKa = 4.91EE171 pKa = 4.16TDD173 pKa = 3.12VKK175 pKa = 10.67SICSFRR181 pKa = 11.84DD182 pKa = 3.2IPVEE186 pKa = 3.76TTMGHH191 pKa = 6.62RR192 pKa = 11.84YY193 pKa = 8.8LALSEE198 pKa = 4.21EE199 pKa = 4.45FASIEE204 pKa = 3.4VDD206 pKa = 3.66YY207 pKa = 10.8QVSSIVTPLNKK218 pKa = 10.14GSIYY222 pKa = 8.04MTLDD226 pKa = 3.1VHH228 pKa = 7.04EE229 pKa = 4.53EE230 pKa = 3.93THH232 pKa = 6.4PKK234 pKa = 10.52SGIQDD239 pKa = 3.33KK240 pKa = 11.27ASIEE244 pKa = 4.0RR245 pKa = 11.84LEE247 pKa = 4.38AAGHH251 pKa = 5.89NALKK255 pKa = 8.55THH257 pKa = 7.21AYY259 pKa = 10.22FDD261 pKa = 3.62DD262 pKa = 4.13SYY264 pKa = 11.85YY265 pKa = 11.02EE266 pKa = 4.16GFEE269 pKa = 3.96EE270 pKa = 5.05SADD273 pKa = 3.76FSSDD277 pKa = 3.39FQRR280 pKa = 11.84LKK282 pKa = 10.48IKK284 pKa = 10.23QSHH287 pKa = 5.11VDD289 pKa = 3.13WYY291 pKa = 11.03KK292 pKa = 11.4DD293 pKa = 3.11PDD295 pKa = 3.77KK296 pKa = 11.13FFEE299 pKa = 4.26PVLNFGASSRR309 pKa = 11.84RR310 pKa = 11.84VGSQKK315 pKa = 9.43TVLTALKK322 pKa = 9.92KK323 pKa = 10.5RR324 pKa = 11.84NADD327 pKa = 3.46VPEE330 pKa = 4.51LSDD333 pKa = 3.7VVDD336 pKa = 3.52ATEE339 pKa = 4.2VPICLRR345 pKa = 11.84QSYY348 pKa = 10.52LNIYY352 pKa = 9.6GADD355 pKa = 3.58CLFEE359 pKa = 4.58SFNIMAKK366 pKa = 10.43GLDD369 pKa = 3.35YY370 pKa = 10.82HH371 pKa = 7.07KK372 pKa = 10.13RR373 pKa = 11.84WKK375 pKa = 9.46SHH377 pKa = 6.68KK378 pKa = 8.6EE379 pKa = 3.86LQGVTLLCEE388 pKa = 4.34TNLQRR393 pKa = 11.84YY394 pKa = 6.05QHH396 pKa = 6.24MIKK399 pKa = 10.28SDD401 pKa = 3.62VKK403 pKa = 9.88PTVTDD408 pKa = 3.51TLHH411 pKa = 6.42VEE413 pKa = 4.03RR414 pKa = 11.84DD415 pKa = 3.51VPATITFHH423 pKa = 6.46GKK425 pKa = 10.12GVTSCFSPFFTACFEE440 pKa = 4.28KK441 pKa = 10.89FSLALRR447 pKa = 11.84EE448 pKa = 4.3SIYY451 pKa = 11.04VPIGKK456 pKa = 9.43ISSLEE461 pKa = 4.12LKK463 pKa = 10.7SKK465 pKa = 10.67ALNNKK470 pKa = 8.84FFLEE474 pKa = 4.34ADD476 pKa = 3.38LSKK479 pKa = 10.63FDD481 pKa = 3.7KK482 pKa = 11.18SQGEE486 pKa = 3.98LHH488 pKa = 7.1LEE490 pKa = 4.13FQRR493 pKa = 11.84LILINLGFPVPLTNWWCDD511 pKa = 3.78FHH513 pKa = 9.59RR514 pKa = 11.84SILSRR519 pKa = 11.84WTLRR523 pKa = 11.84PGVSIPLLSKK533 pKa = 8.57TNRR536 pKa = 11.84GCFHH540 pKa = 7.22LLWEE544 pKa = 4.7HH545 pKa = 6.57FSDD548 pKa = 3.82NAMMAYY554 pKa = 9.79CFDD557 pKa = 3.52MSTAEE562 pKa = 4.07LAMFSGDD569 pKa = 4.01DD570 pKa = 3.53SLVICGSKK578 pKa = 10.68PEE580 pKa = 4.08FDD582 pKa = 3.61PGVFQSLFNMEE593 pKa = 4.61VKK595 pKa = 10.87VMDD598 pKa = 4.34PSLPYY603 pKa = 9.9ICSKK607 pKa = 10.64FLLEE611 pKa = 4.83SEE613 pKa = 4.49FGDD616 pKa = 3.83VFSVPDD622 pKa = 3.89PMRR625 pKa = 11.84EE626 pKa = 3.6IQRR629 pKa = 11.84LQKK632 pKa = 10.53RR633 pKa = 11.84KK634 pKa = 9.4IPKK637 pKa = 9.49DD638 pKa = 3.73VQVLRR643 pKa = 11.84ADD645 pKa = 3.82FDD647 pKa = 4.49SFCDD651 pKa = 3.16RR652 pKa = 11.84MKK654 pKa = 10.89FLDD657 pKa = 3.93RR658 pKa = 11.84LSEE661 pKa = 3.96LSLSVLCRR669 pKa = 11.84LTALKK674 pKa = 10.18YY675 pKa = 9.51CKK677 pKa = 9.71PGIEE681 pKa = 4.06GDD683 pKa = 3.48VRR685 pKa = 11.84AWLSGFAYY693 pKa = 10.27YY694 pKa = 10.4RR695 pKa = 11.84EE696 pKa = 3.88NFLRR700 pKa = 11.84YY701 pKa = 8.99SEE703 pKa = 5.59CYY705 pKa = 8.11VTDD708 pKa = 5.3GIHH711 pKa = 6.97CYY713 pKa = 10.26RR714 pKa = 11.84RR715 pKa = 11.84VDD717 pKa = 3.15PMSRR721 pKa = 11.84FKK723 pKa = 10.88PIDD726 pKa = 3.11KK727 pKa = 9.97FQRR730 pKa = 11.84SDD732 pKa = 3.69KK733 pKa = 10.73EE734 pKa = 4.19WFHH737 pKa = 6.99DD738 pKa = 3.12WRR740 pKa = 11.84NNEE743 pKa = 4.08FPKK746 pKa = 10.9KK747 pKa = 10.12PIDD750 pKa = 3.18KK751 pKa = 8.41MARR754 pKa = 11.84MFGAYY759 pKa = 9.12KK760 pKa = 10.75GPVNNDD766 pKa = 2.63RR767 pKa = 11.84VEE769 pKa = 4.39RR770 pKa = 11.84KK771 pKa = 9.71AKK773 pKa = 10.09YY774 pKa = 9.23KK775 pKa = 10.93VNAAMHH781 pKa = 6.95DD782 pKa = 4.06PFALAYY788 pKa = 8.28EE789 pKa = 4.0RR790 pKa = 11.84RR791 pKa = 11.84YY792 pKa = 9.84VQEE795 pKa = 5.3LEE797 pKa = 4.27LKK799 pKa = 9.83NDD801 pKa = 3.6KK802 pKa = 10.83GNCSVLRR809 pKa = 11.84EE810 pKa = 4.12

Molecular weight: 92.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P24403|MVP_BBMV Movement protein OS=Broad bean mottle virus OX=12301 GN=ORF3a PE=3 SV=1
MM1 pKa = 6.49TTSATGKK8 pKa = 10.21ALNRR12 pKa = 11.84KK13 pKa = 7.38QRR15 pKa = 11.84RR16 pKa = 11.84ALNRR20 pKa = 11.84SNRR23 pKa = 11.84LRR25 pKa = 11.84KK26 pKa = 9.06EE27 pKa = 3.81FQPVIVEE34 pKa = 4.17PLASGQAVSLKK45 pKa = 9.65TRR47 pKa = 11.84TGYY50 pKa = 10.71CVTQFVSNNPEE61 pKa = 3.72VKK63 pKa = 10.05AKK65 pKa = 10.16EE66 pKa = 4.17VVSVSVKK73 pKa = 10.77LPDD76 pKa = 3.73HH77 pKa = 6.46LAVEE81 pKa = 4.77ANRR84 pKa = 11.84ALKK87 pKa = 10.29VGRR90 pKa = 11.84ISILLGLLPTVAGTVKK106 pKa = 10.32VCLTEE111 pKa = 4.7KK112 pKa = 10.21QDD114 pKa = 3.88SPAEE118 pKa = 4.14SFKK121 pKa = 10.95RR122 pKa = 11.84ALAVADD128 pKa = 3.63SSKK131 pKa = 10.78EE132 pKa = 3.91VASAFYY138 pKa = 11.03VDD140 pKa = 3.76GFKK143 pKa = 10.89DD144 pKa = 3.54VSLGDD149 pKa = 3.83LEE151 pKa = 5.63KK152 pKa = 11.13DD153 pKa = 3.28LSIYY157 pKa = 10.34LYY159 pKa = 10.96SEE161 pKa = 4.05AALAANSIRR170 pKa = 11.84IRR172 pKa = 11.84MEE174 pKa = 3.69VEE176 pKa = 3.42HH177 pKa = 6.44VMPKK181 pKa = 10.27FITRR185 pKa = 11.84FSPFAA190 pKa = 5.09

Molecular weight: 20.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2261

190

966

565.3

64.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.678 ± 0.752

2.565 ± 0.462

6.236 ± 0.755

7.298 ± 0.525

4.865 ± 0.606

5.263 ± 0.373

2.433 ± 0.322

4.777 ± 0.384

6.855 ± 0.178

8.536 ± 0.371

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.477 ± 0.218

4.069 ± 0.404

3.671 ± 0.695

2.521 ± 0.094

5.927 ± 0.291

8.89 ± 0.727

4.909 ± 0.432

7.386 ± 0.612

1.327 ± 0.394

3.317 ± 0.289
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski