Amino acid dipepetide frequency for Pseudomonas putida CSV86

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.138AlaAla: 13.138 ± 0.133
1.233AlaCys: 1.233 ± 0.028
5.833AlaAsp: 5.833 ± 0.054
6.964AlaGlu: 6.964 ± 0.074
3.769AlaPhe: 3.769 ± 0.053
9.446AlaGly: 9.446 ± 0.097
2.185AlaHis: 2.185 ± 0.039
5.12AlaIle: 5.12 ± 0.058
3.463AlaLys: 3.463 ± 0.059
13.842AlaLeu: 13.842 ± 0.133
2.836AlaMet: 2.836 ± 0.048
3.056AlaAsn: 3.056 ± 0.05
4.646AlaPro: 4.646 ± 0.059
4.988AlaGln: 4.988 ± 0.061
7.801AlaArg: 7.801 ± 0.08
6.395AlaSer: 6.395 ± 0.067
4.947AlaThr: 4.947 ± 0.063
7.663AlaVal: 7.663 ± 0.072
1.723AlaTrp: 1.723 ± 0.037
2.5AlaTyr: 2.5 ± 0.047
0.0AlaXaa: 0.0 ± 0.0
Cys
1.066CysAla: 1.066 ± 0.031
0.154CysCys: 0.154 ± 0.011
0.53CysAsp: 0.53 ± 0.021
0.519CysGlu: 0.519 ± 0.02
0.338CysPhe: 0.338 ± 0.016
0.945CysGly: 0.945 ± 0.028
0.267CysHis: 0.267 ± 0.015
0.444CysIle: 0.444 ± 0.018
0.286CysLys: 0.286 ± 0.015
1.097CysLeu: 1.097 ± 0.026
0.217CysMet: 0.217 ± 0.013
0.266CysAsn: 0.266 ± 0.014
0.463CysPro: 0.463 ± 0.02
0.407CysGln: 0.407 ± 0.017
0.659CysArg: 0.659 ± 0.021
0.615CysSer: 0.615 ± 0.018
0.493CysThr: 0.493 ± 0.019
0.674CysVal: 0.674 ± 0.017
0.148CysTrp: 0.148 ± 0.01
0.251CysTyr: 0.251 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
5.878AspAla: 5.878 ± 0.064
0.536AspCys: 0.536 ± 0.021
2.943AspAsp: 2.943 ± 0.051
3.503AspGlu: 3.503 ± 0.051
2.095AspPhe: 2.095 ± 0.039
4.677AspGly: 4.677 ± 0.058
1.201AspHis: 1.201 ± 0.028
2.634AspIle: 2.634 ± 0.037
1.869AspLys: 1.869 ± 0.042
6.183AspLeu: 6.183 ± 0.063
1.147AspMet: 1.147 ± 0.027
1.706AspAsn: 1.706 ± 0.037
2.952AspPro: 2.952 ± 0.041
2.153AspGln: 2.153 ± 0.04
3.245AspArg: 3.245 ± 0.039
3.002AspSer: 3.002 ± 0.044
2.425AspThr: 2.425 ± 0.039
3.385AspVal: 3.385 ± 0.061
0.966AspTrp: 0.966 ± 0.025
1.741AspTyr: 1.741 ± 0.035
0.0AspXaa: 0.0 ± 0.0
Glu
6.633GluAla: 6.633 ± 0.072
0.438GluCys: 0.438 ± 0.019
2.601GluAsp: 2.601 ± 0.044
3.259GluGlu: 3.259 ± 0.058
1.798GluPhe: 1.798 ± 0.034
3.957GluGly: 3.957 ± 0.053
1.676GluHis: 1.676 ± 0.039
2.694GluIle: 2.694 ± 0.041
2.086GluLys: 2.086 ± 0.04
6.93GluLeu: 6.93 ± 0.073
1.275GluMet: 1.275 ± 0.026
1.521GluAsn: 1.521 ± 0.029
2.531GluPro: 2.531 ± 0.048
3.749GluGln: 3.749 ± 0.055
4.99GluArg: 4.99 ± 0.071
2.689GluSer: 2.689 ± 0.044
2.539GluThr: 2.539 ± 0.041
4.269GluVal: 4.269 ± 0.064
0.765GluTrp: 0.765 ± 0.021
1.245GluTyr: 1.245 ± 0.03
0.0GluXaa: 0.0 ± 0.0
Phe
4.014PheAla: 4.014 ± 0.055
0.435PheCys: 0.435 ± 0.017
2.478PheAsp: 2.478 ± 0.043
2.139PheGlu: 2.139 ± 0.037
1.403PhePhe: 1.403 ± 0.033
3.179PheGly: 3.179 ± 0.046
0.77PheHis: 0.77 ± 0.022
1.796PheIle: 1.796 ± 0.033
1.24PheLys: 1.24 ± 0.032
3.182PheLeu: 3.182 ± 0.051
0.808PheMet: 0.808 ± 0.022
1.375PheAsn: 1.375 ± 0.031
1.464PhePro: 1.464 ± 0.034
1.188PheGln: 1.188 ± 0.029
1.863PheArg: 1.863 ± 0.034
2.458PheSer: 2.458 ± 0.04
1.858PheThr: 1.858 ± 0.037
2.475PheVal: 2.475 ± 0.04
0.575PheTrp: 0.575 ± 0.02
1.041PheTyr: 1.041 ± 0.029
0.0PheXaa: 0.0 ± 0.0
Gly
7.561GlyAla: 7.561 ± 0.084
0.965GlyCys: 0.965 ± 0.023
4.041GlyAsp: 4.041 ± 0.06
4.912GlyGlu: 4.912 ± 0.061
3.383GlyPhe: 3.383 ± 0.049
6.111GlyGly: 6.111 ± 0.087
1.92GlyHis: 1.92 ± 0.04
4.097GlyIle: 4.097 ± 0.054
3.399GlyLys: 3.399 ± 0.05
9.577GlyLeu: 9.577 ± 0.088
2.162GlyMet: 2.162 ± 0.039
2.559GlyAsn: 2.559 ± 0.074
2.686GlyPro: 2.686 ± 0.046
3.881GlyGln: 3.881 ± 0.052
5.182GlyArg: 5.182 ± 0.052
4.833GlySer: 4.833 ± 0.069
3.861GlyThr: 3.861 ± 0.053
6.01GlyVal: 6.01 ± 0.069
1.359GlyTrp: 1.359 ± 0.032
2.49GlyTyr: 2.49 ± 0.044
0.0GlyXaa: 0.0 ± 0.0
His
2.287HisAla: 2.287 ± 0.04
0.322HisCys: 0.322 ± 0.015
1.31HisAsp: 1.31 ± 0.029
1.226HisGlu: 1.226 ± 0.027
0.974HisPhe: 0.974 ± 0.026
2.107HisGly: 2.107 ± 0.038
0.644HisHis: 0.644 ± 0.025
0.94HisIle: 0.94 ± 0.024
0.631HisLys: 0.631 ± 0.018
2.665HisLeu: 2.665 ± 0.043
0.521HisMet: 0.521 ± 0.018
0.611HisAsn: 0.611 ± 0.02
1.365HisPro: 1.365 ± 0.026
0.93HisGln: 0.93 ± 0.026
1.397HisArg: 1.397 ± 0.028
1.297HisSer: 1.297 ± 0.028
0.932HisThr: 0.932 ± 0.025
1.332HisVal: 1.332 ± 0.028
0.463HisTrp: 0.463 ± 0.016
0.765HisTyr: 0.765 ± 0.019
0.0HisXaa: 0.0 ± 0.0
Ile
5.788IleAla: 5.788 ± 0.07
0.495IleCys: 0.495 ± 0.019
3.39IleAsp: 3.39 ± 0.044
3.205IleGlu: 3.205 ± 0.047
1.346IlePhe: 1.346 ± 0.032
4.446IleGly: 4.446 ± 0.057
0.956IleHis: 0.956 ± 0.026
1.886IleIle: 1.886 ± 0.039
1.618IleLys: 1.618 ± 0.035
4.015IleLeu: 4.015 ± 0.054
0.804IleMet: 0.804 ± 0.025
1.638IleAsn: 1.638 ± 0.036
2.157IlePro: 2.157 ± 0.044
1.62IleGln: 1.62 ± 0.032
2.906IleArg: 2.906 ± 0.044
2.862IleSer: 2.862 ± 0.044
2.475IleThr: 2.475 ± 0.044
3.12IleVal: 3.12 ± 0.049
0.497IleTrp: 0.497 ± 0.022
1.046IleTyr: 1.046 ± 0.023
0.0IleXaa: 0.0 ± 0.0
Lys
4.134LysAla: 4.134 ± 0.053
0.167LysCys: 0.167 ± 0.01
1.797LysAsp: 1.797 ± 0.037
1.668LysGlu: 1.668 ± 0.038
0.842LysPhe: 0.842 ± 0.021
2.617LysGly: 2.617 ± 0.048
0.767LysHis: 0.767 ± 0.022
1.516LysIle: 1.516 ± 0.033
1.29LysLys: 1.29 ± 0.037
3.483LysLeu: 3.483 ± 0.046
0.681LysMet: 0.681 ± 0.024
0.981LysAsn: 0.981 ± 0.025
2.044LysPro: 2.044 ± 0.042
1.456LysGln: 1.456 ± 0.03
2.37LysArg: 2.37 ± 0.039
1.739LysSer: 1.739 ± 0.041
1.808LysThr: 1.808 ± 0.032
2.811LysVal: 2.811 ± 0.049
0.357LysTrp: 0.357 ± 0.016
0.698LysTyr: 0.698 ± 0.021
0.0LysXaa: 0.0 ± 0.0
Leu
14.328LeuAla: 14.328 ± 0.124
1.18LeuCys: 1.18 ± 0.028
7.132LeuAsp: 7.132 ± 0.079
6.619LeuGlu: 6.619 ± 0.078
4.142LeuPhe: 4.142 ± 0.056
9.584LeuGly: 9.584 ± 0.079
2.52LeuHis: 2.52 ± 0.04
5.181LeuIle: 5.181 ± 0.066
4.223LeuLys: 4.223 ± 0.051
13.294LeuLeu: 13.294 ± 0.159
2.444LeuMet: 2.444 ± 0.042
3.503LeuAsn: 3.503 ± 0.051
6.36LeuPro: 6.36 ± 0.078
4.622LeuGln: 4.622 ± 0.067
7.744LeuArg: 7.744 ± 0.092
7.094LeuSer: 7.094 ± 0.07
5.46LeuThr: 5.46 ± 0.063
8.164LeuVal: 8.164 ± 0.082
1.372LeuTrp: 1.372 ± 0.031
2.613LeuTyr: 2.613 ± 0.042
0.0LeuXaa: 0.0 ± 0.0
Met
2.556MetAla: 2.556 ± 0.04
0.178MetCys: 0.178 ± 0.013
1.035MetAsp: 1.035 ± 0.026
0.955MetGlu: 0.955 ± 0.027
0.677MetPhe: 0.677 ± 0.021
1.6MetGly: 1.6 ± 0.033
0.48MetHis: 0.48 ± 0.018
1.115MetIle: 1.115 ± 0.027
0.892MetLys: 0.892 ± 0.024
2.619MetLeu: 2.619 ± 0.05
0.476MetMet: 0.476 ± 0.024
0.846MetAsn: 0.846 ± 0.021
1.335MetPro: 1.335 ± 0.028
1.032MetGln: 1.032 ± 0.028
1.511MetArg: 1.511 ± 0.036
1.726MetSer: 1.726 ± 0.033
1.432MetThr: 1.432 ± 0.034
1.484MetVal: 1.484 ± 0.034
0.181MetTrp: 0.181 ± 0.012
0.345MetTyr: 0.345 ± 0.015
0.0MetXaa: 0.0 ± 0.0
Asn
3.264AsnAla: 3.264 ± 0.049
0.279AsnCys: 0.279 ± 0.013
1.601AsnAsp: 1.601 ± 0.037
1.467AsnGlu: 1.467 ± 0.035
1.065AsnPhe: 1.065 ± 0.027
2.753AsnGly: 2.753 ± 0.062
0.658AsnHis: 0.658 ± 0.022
1.38AsnIle: 1.38 ± 0.031
0.89AsnLys: 0.89 ± 0.027
3.545AsnLeu: 3.545 ± 0.057
0.556AsnMet: 0.556 ± 0.015
0.936AsnAsn: 0.936 ± 0.029
1.981AsnPro: 1.981 ± 0.039
1.277AsnGln: 1.277 ± 0.032
1.99AsnArg: 1.99 ± 0.034
1.54AsnSer: 1.54 ± 0.037
1.457AsnThr: 1.457 ± 0.034
1.958AsnVal: 1.958 ± 0.045
0.483AsnTrp: 0.483 ± 0.019
0.813AsnTyr: 0.813 ± 0.021
0.0AsnXaa: 0.0 ± 0.0
Pro
5.887ProAla: 5.887 ± 0.069
0.386ProCys: 0.386 ± 0.014
2.801ProAsp: 2.801 ± 0.046
3.213ProGlu: 3.213 ± 0.052
1.805ProPhe: 1.805 ± 0.032
4.215ProGly: 4.215 ± 0.061
1.051ProHis: 1.051 ± 0.026
1.871ProIle: 1.871 ± 0.036
1.536ProLys: 1.536 ± 0.032
5.716ProLeu: 5.716 ± 0.076
1.114ProMet: 1.114 ± 0.029
1.32ProAsn: 1.32 ± 0.03
1.937ProPro: 1.937 ± 0.047
2.276ProGln: 2.276 ± 0.036
2.71ProArg: 2.71 ± 0.044
2.558ProSer: 2.558 ± 0.045
2.173ProThr: 2.173 ± 0.038
3.908ProVal: 3.908 ± 0.046
0.797ProTrp: 0.797 ± 0.021
1.171ProTyr: 1.171 ± 0.026
0.0ProXaa: 0.0 ± 0.0
Gln
6.008GlnAla: 6.008 ± 0.072
0.326GlnCys: 0.326 ± 0.014
1.961GlnAsp: 1.961 ± 0.036
1.915GlnGlu: 1.915 ± 0.036
1.29GlnPhe: 1.29 ± 0.029
3.455GlnGly: 3.455 ± 0.046
1.054GlnHis: 1.054 ± 0.025
1.981GlnIle: 1.981 ± 0.035
1.237GlnLys: 1.237 ± 0.03
5.336GlnLeu: 5.336 ± 0.068
1.035GlnMet: 1.035 ± 0.026
1.056GlnAsn: 1.056 ± 0.027
2.385GlnPro: 2.385 ± 0.041
2.68GlnGln: 2.68 ± 0.051
3.853GlnArg: 3.853 ± 0.057
2.221GlnSer: 2.221 ± 0.044
1.921GlnThr: 1.921 ± 0.037
3.685GlnVal: 3.685 ± 0.055
0.727GlnTrp: 0.727 ± 0.022
1.011GlnTyr: 1.011 ± 0.028
0.0GlnXaa: 0.0 ± 0.0
Arg
6.267ArgAla: 6.267 ± 0.068
0.63ArgCys: 0.63 ± 0.019
3.685ArgAsp: 3.685 ± 0.054
4.333ArgGlu: 4.333 ± 0.062
2.849ArgPhe: 2.849 ± 0.046
4.251ArgGly: 4.251 ± 0.058
1.869ArgHis: 1.869 ± 0.039
3.527ArgIle: 3.527 ± 0.044
2.151ArgLys: 2.151 ± 0.039
8.858ArgLeu: 8.858 ± 0.099
1.716ArgMet: 1.716 ± 0.033
2.066ArgAsn: 2.066 ± 0.037
2.888ArgPro: 2.888 ± 0.05
3.685ArgGln: 3.685 ± 0.061
4.72ArgArg: 4.72 ± 0.067
3.724ArgSer: 3.724 ± 0.058
2.886ArgThr: 2.886 ± 0.044
4.523ArgVal: 4.523 ± 0.056
1.112ArgTrp: 1.112 ± 0.024
2.116ArgTyr: 2.116 ± 0.04
0.0ArgXaa: 0.0 ± 0.0
Ser
6.085SerAla: 6.085 ± 0.06
0.467SerCys: 0.467 ± 0.017
2.841SerAsp: 2.841 ± 0.041
3.112SerGlu: 3.112 ± 0.051
2.16SerPhe: 2.16 ± 0.04
5.303SerGly: 5.303 ± 0.069
1.291SerHis: 1.291 ± 0.029
2.826SerIle: 2.826 ± 0.045
1.75SerLys: 1.75 ± 0.033
7.173SerLeu: 7.173 ± 0.066
1.365SerMet: 1.365 ± 0.03
1.728SerAsn: 1.728 ± 0.038
2.789SerPro: 2.789 ± 0.044
2.379SerGln: 2.379 ± 0.04
3.796SerArg: 3.796 ± 0.053
3.521SerSer: 3.521 ± 0.055
2.903SerThr: 2.903 ± 0.044
4.035SerVal: 4.035 ± 0.048
0.77SerTrp: 0.77 ± 0.022
1.505SerTyr: 1.505 ± 0.036
0.0SerXaa: 0.0 ± 0.0
Thr
5.139ThrAla: 5.139 ± 0.069
0.472ThrCys: 0.472 ± 0.014
2.323ThrAsp: 2.323 ± 0.038
2.317ThrGlu: 2.317 ± 0.036
1.758ThrPhe: 1.758 ± 0.035
4.122ThrGly: 4.122 ± 0.062
1.033ThrHis: 1.033 ± 0.024
1.892ThrIle: 1.892 ± 0.034
1.071ThrLys: 1.071 ± 0.025
6.563ThrLeu: 6.563 ± 0.071
0.801ThrMet: 0.801 ± 0.022
1.218ThrAsn: 1.218 ± 0.031
3.028ThrPro: 3.028 ± 0.046
1.822ThrGln: 1.822 ± 0.036
3.213ThrArg: 3.213 ± 0.037
2.74ThrSer: 2.74 ± 0.047
2.434ThrThr: 2.434 ± 0.054
3.572ThrVal: 3.572 ± 0.058
0.786ThrTrp: 0.786 ± 0.022
1.277ThrTyr: 1.277 ± 0.034
0.0ThrXaa: 0.0 ± 0.0
Val
7.798ValAla: 7.798 ± 0.076
0.722ValCys: 0.722 ± 0.022
3.874ValAsp: 3.874 ± 0.046
4.489ValGlu: 4.489 ± 0.059
2.548ValPhe: 2.548 ± 0.045
5.257ValGly: 5.257 ± 0.059
1.427ValHis: 1.427 ± 0.029
3.699ValIle: 3.699 ± 0.056
2.358ValLys: 2.358 ± 0.041
8.259ValLeu: 8.259 ± 0.083
1.687ValMet: 1.687 ± 0.035
2.252ValAsn: 2.252 ± 0.041
3.438ValPro: 3.438 ± 0.042
2.826ValGln: 2.826 ± 0.038
4.625ValArg: 4.625 ± 0.056
4.327ValSer: 4.327 ± 0.059
3.642ValThr: 3.642 ± 0.049
5.44ValVal: 5.44 ± 0.069
0.864ValTrp: 0.864 ± 0.026
1.573ValTyr: 1.573 ± 0.03
0.0ValXaa: 0.0 ± 0.0
Trp
1.209TrpAla: 1.209 ± 0.03
0.169TrpCys: 0.169 ± 0.01
0.638TrpAsp: 0.638 ± 0.02
0.618TrpGlu: 0.618 ± 0.018
0.56TrpPhe: 0.56 ± 0.017
0.929TrpGly: 0.929 ± 0.025
0.403TrpHis: 0.403 ± 0.016
0.708TrpIle: 0.708 ± 0.022
0.479TrpLys: 0.479 ± 0.016
2.179TrpLeu: 2.179 ± 0.042
0.362TrpMet: 0.362 ± 0.017
0.492TrpAsn: 0.492 ± 0.015
0.686TrpPro: 0.686 ± 0.021
0.901TrpGln: 0.901 ± 0.024
1.169TrpArg: 1.169 ± 0.028
0.87TrpSer: 0.87 ± 0.022
0.701TrpThr: 0.701 ± 0.022
0.952TrpVal: 0.952 ± 0.026
0.255TrpTrp: 0.255 ± 0.014
0.354TrpTyr: 0.354 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.506TyrAla: 2.506 ± 0.039
0.286TyrCys: 0.286 ± 0.014
1.427TyrAsp: 1.427 ± 0.035
1.215TyrGlu: 1.215 ± 0.029
0.943TyrPhe: 0.943 ± 0.024
2.035TyrGly: 2.035 ± 0.04
0.548TyrHis: 0.548 ± 0.017
0.963TyrIle: 0.963 ± 0.026
0.755TyrLys: 0.755 ± 0.025
3.059TyrLeu: 3.059 ± 0.044
0.469TyrMet: 0.469 ± 0.018
0.763TyrAsn: 0.763 ± 0.023
1.308TyrPro: 1.308 ± 0.027
1.263TyrGln: 1.263 ± 0.03
2.111TyrArg: 2.111 ± 0.036
1.589TyrSer: 1.589 ± 0.034
1.236TyrThr: 1.236 ± 0.03
1.646TyrVal: 1.646 ± 0.033
0.433TyrTrp: 0.433 ± 0.016
0.717TyrTyr: 0.717 ± 0.023
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5175 proteins (1631647 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski