Pseudomonas phage Phabio

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Phikzvirus; unclassified Phikzvirus

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 468 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y0STA7|A0A1Y0STA7_9CAUD Uncharacterized protein OS=Pseudomonas phage Phabio OX=2006668 GN=PHABIO_80 PE=4 SV=1
MM1 pKa = 7.34EE2 pKa = 5.34VIADD6 pKa = 4.14ICQLSLSDD14 pKa = 3.93LTDD17 pKa = 3.66FAIAYY22 pKa = 9.57VEE24 pKa = 4.14YY25 pKa = 10.28EE26 pKa = 4.08DD27 pKa = 4.94VGGNFMNDD35 pKa = 3.12DD36 pKa = 4.99DD37 pKa = 5.69LVQDD41 pKa = 3.7YY42 pKa = 10.9FGGKK46 pKa = 8.21NVGYY50 pKa = 9.84RR51 pKa = 11.84VIEE54 pKa = 4.31IIRR57 pKa = 11.84AQRR60 pKa = 11.84CEE62 pKa = 3.51IMAYY66 pKa = 9.39MPDD69 pKa = 3.3NVVEE73 pKa = 4.18MKK75 pKa = 10.07YY76 pKa = 10.82ACTRR80 pKa = 11.84GDD82 pKa = 3.52GVFVYY87 pKa = 10.65ARR89 pKa = 11.84TANDD93 pKa = 3.23VNVGG97 pKa = 3.43

Molecular weight: 10.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y0SWH4|A0A1Y0SWH4_9CAUD Uncharacterized protein OS=Pseudomonas phage Phabio OX=2006668 GN=PHABIO_303 PE=4 SV=1
MM1 pKa = 7.19YY2 pKa = 10.02DD3 pKa = 3.19KK4 pKa = 11.37VSILKK9 pKa = 9.46QVNVTASNSTSPIGEE24 pKa = 4.15APDD27 pKa = 3.57LKK29 pKa = 10.66GISVGEE35 pKa = 4.2LIAAARR41 pKa = 11.84ARR43 pKa = 11.84SNNTAVAQLVDD54 pKa = 3.8WASKK58 pKa = 9.95FPPSKK63 pKa = 10.15RR64 pKa = 11.84RR65 pKa = 11.84SLYY68 pKa = 10.16RR69 pKa = 11.84RR70 pKa = 11.84ATAPIRR76 pKa = 11.84RR77 pKa = 11.84QWHH80 pKa = 6.37RR81 pKa = 11.84IKK83 pKa = 10.33ATYY86 pKa = 10.16NYY88 pKa = 7.43WKK90 pKa = 10.6NYY92 pKa = 8.27

Molecular weight: 10.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

468

0

468

97552

18

2326

208.4

23.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.885 ± 0.164

0.979 ± 0.047

6.491 ± 0.109

6.712 ± 0.13

4.121 ± 0.067

6.282 ± 0.163

2.119 ± 0.07

6.253 ± 0.099

6.097 ± 0.112

8.439 ± 0.099

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.776 ± 0.07

5.189 ± 0.103

4.139 ± 0.074

3.634 ± 0.079

5.102 ± 0.094

5.679 ± 0.102

6.455 ± 0.093

7.163 ± 0.107

1.336 ± 0.051

4.15 ± 0.088
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski