Macaca mulatta feces associated virus 10
Average proteome isoelectric point is 7.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W5PX53|A0A1W5PX53_9VIRU Cap OS=Macaca mulatta feces associated virus 10 OX=2499232 PE=4 SV=1
MM1 pKa = 7.96 PILLGMRR8 pKa = 11.84 FLCIDD13 pKa = 3.88 IGCEE17 pKa = 4.2 IMTTTQFASASYY29 pKa = 10.78 QEE31 pKa = 5.1 VIDD34 pKa = 5.17 LSTTGDD40 pKa = 3.45 TVSAIGIHH48 pKa = 5.61 TPCSATPVDD57 pKa = 4.02 MLSGFWRR64 pKa = 11.84 QFQKK68 pKa = 10.52 VRR70 pKa = 11.84 YY71 pKa = 8.55 DD72 pKa = 3.44 GCSVTLVPAARR83 pKa = 11.84 LPVDD87 pKa = 3.59 PLGVSYY93 pKa = 10.41 EE94 pKa = 4.09 AGEE97 pKa = 4.05 ATIDD101 pKa = 4.58 PRR103 pKa = 11.84 DD104 pKa = 3.9 LLNPILFHH112 pKa = 6.51 GCHH115 pKa = 6.26 GNDD118 pKa = 2.85 MGYY121 pKa = 9.85 ILNQLYY127 pKa = 10.93 SGDD130 pKa = 3.77 ATISEE135 pKa = 4.09 AWMQKK140 pKa = 9.89 VGASDD145 pKa = 3.38 STEE148 pKa = 4.01 QLNMIADD155 pKa = 4.12 GAEE158 pKa = 4.38 SINTVAEE165 pKa = 3.8 ALYY168 pKa = 10.87 YY169 pKa = 10.38 KK170 pKa = 10.8 ALTDD174 pKa = 3.94 NTWKK178 pKa = 10.76 KK179 pKa = 10.93 ADD181 pKa = 3.66 PQRR184 pKa = 11.84 GFKK187 pKa = 10.51 KK188 pKa = 10.36 SGLRR192 pKa = 11.84 PLVHH196 pKa = 7.23 DD197 pKa = 4.74 LAVNVPFGVGKK208 pKa = 9.85 HH209 pKa = 5.9 LGAEE213 pKa = 4.34 VTGTSASIQNGRR225 pKa = 11.84 FATASPTHH233 pKa = 6.55 PAGSGNDD240 pKa = 3.84 DD241 pKa = 4.4 SIQTSDD247 pKa = 4.72 LGFLNTGFNSLGFPDD262 pKa = 4.27 TTWTPIIGQNPGKK275 pKa = 10.27 LVIGSNPNGIQFMTNRR291 pKa = 11.84 LRR293 pKa = 11.84 SLGWLDD299 pKa = 3.32 TRR301 pKa = 11.84 SPLARR306 pKa = 11.84 IQTGSSGVTIPSGSRR321 pKa = 11.84 AGDD324 pKa = 3.28 IGPSTPSGPSRR335 pKa = 11.84 SVTDD339 pKa = 3.46 VYY341 pKa = 11.64 AKK343 pKa = 10.7 VIAEE347 pKa = 4.54 DD348 pKa = 4.12 GNTARR353 pKa = 11.84 AVEE356 pKa = 4.03 NTIPKK361 pKa = 9.78 IFMGMILLPPAYY373 pKa = 8.29 KK374 pKa = 9.3 TKK376 pKa = 10.4 QYY378 pKa = 10.49 FRR380 pKa = 11.84 MVINHH385 pKa = 6.71 RR386 pKa = 11.84 FSFAKK391 pKa = 10.18 FRR393 pKa = 11.84 GISMNNMSTDD403 pKa = 3.77 EE404 pKa = 4.24 VKK406 pKa = 10.44 SAYY409 pKa = 9.98 AYY411 pKa = 10.25 YY412 pKa = 10.8 DD413 pKa = 3.48 NYY415 pKa = 11.42 SSS417 pKa = 3.69
Molecular weight: 44.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.684
IPC2_protein 5.677
IPC_protein 5.715
Toseland 6.084
ProMoST 6.046
Dawson 5.982
Bjellqvist 5.982
Wikipedia 5.995
Rodwell 5.957
Grimsley 6.224
Solomon 5.982
Lehninger 5.97
Nozaki 6.237
DTASelect 6.44
Thurlkill 6.427
EMBOSS 6.402
Sillero 6.338
Patrickios 4.012
IPC_peptide 5.995
IPC2_peptide 6.326
IPC2.peptide.svr19 6.3
Protein with the highest isoelectric point:
>tr|A0A1W5PX53|A0A1W5PX53_9VIRU Cap OS=Macaca mulatta feces associated virus 10 OX=2499232 PE=4 SV=1
MM1 pKa = 7.37 IMSKK5 pKa = 8.69 TFMLTIPRR13 pKa = 11.84 NDD15 pKa = 3.34 TYY17 pKa = 11.48 RR18 pKa = 11.84 EE19 pKa = 4.08 GNRR22 pKa = 11.84 IFAWFRR28 pKa = 11.84 KK29 pKa = 9.34 NDD31 pKa = 2.9 IHH33 pKa = 7.66 KK34 pKa = 9.66 WVLGAEE40 pKa = 4.24 KK41 pKa = 10.49 GAGGYY46 pKa = 7.24 EE47 pKa = 3.88 HH48 pKa = 6.81 WQMRR52 pKa = 11.84 VQCRR56 pKa = 11.84 FGFEE60 pKa = 3.86 KK61 pKa = 10.89 LKK63 pKa = 11.0 VLFPNAHH70 pKa = 6.49 IEE72 pKa = 4.12 EE73 pKa = 5.17 CSDD76 pKa = 2.26 KK77 pKa = 9.3 WTYY80 pKa = 6.97 EE81 pKa = 4.19 TKK83 pKa = 10.67 EE84 pKa = 3.83 GVYY87 pKa = 9.18 WKK89 pKa = 11.14 SNDD92 pKa = 3.33 RR93 pKa = 11.84 EE94 pKa = 4.2 EE95 pKa = 4.28 NRR97 pKa = 11.84 KK98 pKa = 8.65 QRR100 pKa = 11.84 FGKK103 pKa = 10.02 LRR105 pKa = 11.84 YY106 pKa = 7.83 AQKK109 pKa = 10.56 RR110 pKa = 11.84 VIEE113 pKa = 4.52 HH114 pKa = 7.36 ADD116 pKa = 3.33 GTNDD120 pKa = 3.42 RR121 pKa = 11.84 EE122 pKa = 4.38 VVVWYY127 pKa = 9.72 DD128 pKa = 3.37 QEE130 pKa = 4.44 GKK132 pKa = 10.31 AGKK135 pKa = 9.48 SWLTGAMWEE144 pKa = 4.12 RR145 pKa = 11.84 GLAYY149 pKa = 9.93 FAVADD154 pKa = 4.1 TSGKK158 pKa = 8.4 TIVMDD163 pKa = 4.01 IASEE167 pKa = 4.03 YY168 pKa = 10.46 LKK170 pKa = 11.03 NGWRR174 pKa = 11.84 PYY176 pKa = 10.68 VIIDD180 pKa = 3.52 IPRR183 pKa = 11.84 AGKK186 pKa = 7.17 WTVIVMTNTMPKK198 pKa = 9.79 LDD200 pKa = 4.46 KK201 pKa = 11.01 LSADD205 pKa = 2.96 RR206 pKa = 11.84 WKK208 pKa = 10.62 IITQEE213 pKa = 3.96 EE214 pKa = 4.52 LTLEE218 pKa = 4.07 EE219 pKa = 3.89 TAIEE223 pKa = 4.08 QFALTGKK230 pKa = 10.13 GGYY233 pKa = 9.12 RR234 pKa = 11.84 PPLTPPTT241 pKa = 3.94
Molecular weight: 28.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.582
IPC2_protein 8.492
IPC_protein 8.434
Toseland 9.107
ProMoST 8.902
Dawson 9.399
Bjellqvist 9.151
Wikipedia 9.56
Rodwell 9.619
Grimsley 9.458
Solomon 9.472
Lehninger 9.428
Nozaki 9.224
DTASelect 9.107
Thurlkill 9.238
EMBOSS 9.545
Sillero 9.355
Patrickios 4.851
IPC_peptide 9.458
IPC2_peptide 7.717
IPC2.peptide.svr19 7.656
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
658
241
417
329.0
36.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.447 ± 0.45
1.064 ± 0.13
5.623 ± 0.359
4.863 ± 1.914
3.951 ± 0.121
8.967 ± 0.603
1.672 ± 0.007
6.535 ± 0.173
5.471 ± 1.807
6.687 ± 0.489
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.191 ± 0.071
4.559 ± 0.46
5.167 ± 0.798
3.191 ± 0.16
5.319 ± 0.967
6.839 ± 2.192
7.903 ± 0.242
5.471 ± 0.043
2.432 ± 1.188
3.647 ± 0.28
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here