Conexivisphaera calida
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1603 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P2VNS0|A0A4P2VNS0_9ARCH Molybdenum cofactor biosynthesis protein MoaC OS=Conexivisphaera calida OX=1874277 GN=NAS2_1166 PE=4 SV=1
MM1 pKa = 7.6 LGALILIVIVLVGAFIVYY19 pKa = 9.95 SYY21 pKa = 10.84 VWSASGRR28 pKa = 11.84 ITGAPGVMVSGASLTALPGGGGYY51 pKa = 7.21 MTVDD55 pKa = 3.19 ITNTGGVTGFASVAVYY71 pKa = 10.45 SSSGQVFSAPGAPGLLYY88 pKa = 10.21 QIYY91 pKa = 9.47 YY92 pKa = 9.88 IPTAWYY98 pKa = 9.78 QPDD101 pKa = 3.47 PTAVYY106 pKa = 10.32 SAGLAAGSSFSSDD119 pKa = 2.29 GFTWVASAGPWTTAQSGSSQNVNGQTQPNVIDD151 pKa = 4.02 NLQAGYY157 pKa = 10.47 SGGAPFPNPPVANGWDD173 pKa = 3.11 AYY175 pKa = 10.33 AIKK178 pKa = 10.39 EE179 pKa = 3.74 IGYY182 pKa = 9.48 VVVTQPTTFYY192 pKa = 11.37 VDD194 pKa = 2.8 IDD196 pKa = 3.81 GGLLSVEE203 pKa = 4.48 PLSQGGASLTNWLGTGSNPGNLINQWRR230 pKa = 11.84 GEE232 pKa = 3.98 GATQYY237 pKa = 11.23 SSNTVQPGTYY247 pKa = 9.64 LIEE250 pKa = 4.25 YY251 pKa = 9.74 DD252 pKa = 3.51 WFNGGGPAYY261 pKa = 10.39 FSLWTNNPVQYY272 pKa = 10.54 YY273 pKa = 10.19 SPLQIAPGQVANEE286 pKa = 4.44 SYY288 pKa = 10.33 TVGSGIAPGDD298 pKa = 3.79 TYY300 pKa = 11.08 TVVVTLTTPRR310 pKa = 11.84 GTATSSATVVASPP323 pKa = 4.18
Molecular weight: 33.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.886
IPC2_protein 3.719
IPC_protein 3.656
Toseland 3.452
ProMoST 3.846
Dawson 3.668
Bjellqvist 3.834
Wikipedia 3.63
Rodwell 3.49
Grimsley 3.363
Solomon 3.63
Lehninger 3.592
Nozaki 3.808
DTASelect 4.024
Thurlkill 3.528
EMBOSS 3.643
Sillero 3.783
Patrickios 0.006
IPC_peptide 3.63
IPC2_peptide 3.757
IPC2.peptide.svr19 3.733
Protein with the highest isoelectric point:
>tr|A0A4P2VBB3|A0A4P2VBB3_9ARCH FIG006285: ICC-like protein phosphoesterase OS=Conexivisphaera calida OX=1874277 GN=NAS2_0436 PE=4 SV=1
MM1 pKa = 7.48 KK2 pKa = 10.57 GKK4 pKa = 10.21 GVQVWKK10 pKa = 10.13 YY11 pKa = 8.62 YY12 pKa = 10.11 KK13 pKa = 10.53 VEE15 pKa = 4.26 GGTLTRR21 pKa = 11.84 LRR23 pKa = 11.84 RR24 pKa = 11.84 EE25 pKa = 4.46 CPRR28 pKa = 11.84 CGPGVFMAQHH38 pKa = 7.28 ADD40 pKa = 3.15 RR41 pKa = 11.84 LYY43 pKa = 10.8 CGRR46 pKa = 11.84 CGHH49 pKa = 5.79 TMWLSSSSGSKK60 pKa = 10.42 SSGQRR65 pKa = 11.84 GTGRR69 pKa = 11.84 GRR71 pKa = 11.84 RR72 pKa = 11.84 QQ73 pKa = 2.83
Molecular weight: 8.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.338
IPC2_protein 9.853
IPC_protein 10.76
Toseland 10.833
ProMoST 10.613
Dawson 10.921
Bjellqvist 10.687
Wikipedia 11.169
Rodwell 11.096
Grimsley 10.979
Solomon 11.082
Lehninger 11.038
Nozaki 10.847
DTASelect 10.672
Thurlkill 10.833
EMBOSS 11.242
Sillero 10.862
Patrickios 10.862
IPC_peptide 11.082
IPC2_peptide 10.014
IPC2.peptide.svr19 8.374
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1603
0
1603
468978
37
1935
292.6
32.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.185 ± 0.076
0.666 ± 0.023
5.052 ± 0.05
6.841 ± 0.077
3.107 ± 0.034
9.09 ± 0.052
1.609 ± 0.024
5.19 ± 0.038
3.081 ± 0.057
10.146 ± 0.072
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.809 ± 0.028
2.241 ± 0.04
5.092 ± 0.046
1.815 ± 0.031
8.104 ± 0.094
7.128 ± 0.076
4.193 ± 0.067
8.91 ± 0.056
1.232 ± 0.029
3.509 ± 0.048
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here