Jeotgalibacillus malaysiensis
Average proteome isoelectric point is 6.05
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4426 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B5AN96|A0A0B5AN96_9BACL D-alanine--D-alanine ligase OS=Jeotgalibacillus malaysiensis OX=1508404 GN=ddl PE=3 SV=1
MM1 pKa = 7.62 KK2 pKa = 10.43 KK3 pKa = 10.5 LLFPVIAGALVLGACGSEE21 pKa = 4.09 EE22 pKa = 3.99 NAEE25 pKa = 4.33 TTTGSAAEE33 pKa = 4.19 KK34 pKa = 10.37 EE35 pKa = 4.5 EE36 pKa = 3.97 MTISHH41 pKa = 7.6 LLGDD45 pKa = 4.0 TTVEE49 pKa = 3.99 KK50 pKa = 10.75 SPEE53 pKa = 4.16 SVVVFDD59 pKa = 6.09 FGTLDD64 pKa = 3.28 TLDD67 pKa = 3.79 ALGVEE72 pKa = 4.87 VAGVPQTNIPSYY84 pKa = 8.87 LSKK87 pKa = 11.5 YY88 pKa = 9.63 EE89 pKa = 4.14 GEE91 pKa = 4.5 DD92 pKa = 3.53 YY93 pKa = 11.42 EE94 pKa = 4.44 NAGGLKK100 pKa = 10.32 EE101 pKa = 4.4 PDD103 pKa = 3.37 FEE105 pKa = 7.27 AINAMDD111 pKa = 5.57 PDD113 pKa = 5.02 LIIISGRR120 pKa = 11.84 QADD123 pKa = 4.15 LYY125 pKa = 11.36 EE126 pKa = 4.47 EE127 pKa = 4.73 FEE129 pKa = 5.17 AIAPTIYY136 pKa = 10.02 MGVDD140 pKa = 3.02 TEE142 pKa = 4.95 NYY144 pKa = 8.7 MEE146 pKa = 4.32 SFEE149 pKa = 5.12 EE150 pKa = 4.32 NVNTLAALFEE160 pKa = 4.41 KK161 pKa = 10.8 EE162 pKa = 3.5 EE163 pKa = 3.99 AAAEE167 pKa = 4.03 KK168 pKa = 10.42 LDD170 pKa = 4.62 EE171 pKa = 4.8 INVSIDD177 pKa = 3.38 EE178 pKa = 4.56 LNEE181 pKa = 3.9 QVTSQDD187 pKa = 3.1 LEE189 pKa = 4.32 ALVTLANDD197 pKa = 4.45 GKK199 pKa = 10.57 ISAYY203 pKa = 10.24 GAGSRR208 pKa = 11.84 FGIIHH213 pKa = 7.04 DD214 pKa = 3.85 VFGFTPADD222 pKa = 3.48 EE223 pKa = 4.69 TIEE226 pKa = 4.17 VSTHH230 pKa = 4.87 GQSISYY236 pKa = 9.54 EE237 pKa = 4.28 YY238 pKa = 10.19 IVEE241 pKa = 4.33 EE242 pKa = 4.28 DD243 pKa = 3.25 PQYY246 pKa = 11.3 IFVVDD251 pKa = 4.52 RR252 pKa = 11.84 GAAVDD257 pKa = 4.24 GEE259 pKa = 4.54 SSAKK263 pKa = 9.74 QVVEE267 pKa = 3.91 NEE269 pKa = 4.5 FIEE272 pKa = 4.78 GTQAYY277 pKa = 8.5 EE278 pKa = 3.94 NGNIIYY284 pKa = 10.15 LDD286 pKa = 3.54 PDD288 pKa = 3.14 YY289 pKa = 10.58 WYY291 pKa = 10.92 LSGGGLVSVQEE302 pKa = 4.45 MVNEE306 pKa = 3.95 IQSGIEE312 pKa = 3.66
Molecular weight: 33.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.728
IPC2_protein 3.757
IPC_protein 3.732
Toseland 3.554
ProMoST 3.846
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.567
Rodwell 3.567
Grimsley 3.452
Solomon 3.681
Lehninger 3.63
Nozaki 3.795
DTASelect 3.935
Thurlkill 3.567
EMBOSS 3.579
Sillero 3.846
Patrickios 1.202
IPC_peptide 3.681
IPC2_peptide 3.834
IPC2.peptide.svr19 3.76
Protein with the highest isoelectric point:
>tr|A0A0B5ALD0|A0A0B5ALD0_9BACL tRNA dimethylallyltransferase OS=Jeotgalibacillus malaysiensis OX=1508404 GN=miaA PE=3 SV=1
MM1 pKa = 8.08 AITQEE6 pKa = 4.12 RR7 pKa = 11.84 KK8 pKa = 10.14 NEE10 pKa = 4.02 IIQEE14 pKa = 4.13 FRR16 pKa = 11.84 THH18 pKa = 6.56 EE19 pKa = 4.34 GDD21 pKa = 3.3 TGSVEE26 pKa = 4.13 VQVAILTEE34 pKa = 4.94 DD35 pKa = 3.29 INNLNQHH42 pKa = 5.96 LRR44 pKa = 11.84 THH46 pKa = 6.54 KK47 pKa = 10.02 KK48 pKa = 9.14 DD49 pKa = 3.03 HH50 pKa = 6.33 HH51 pKa = 6.06 SRR53 pKa = 11.84 RR54 pKa = 11.84 GLFKK58 pKa = 10.02 MVGRR62 pKa = 11.84 RR63 pKa = 11.84 RR64 pKa = 11.84 NLLTYY69 pKa = 10.47 LRR71 pKa = 11.84 NNDD74 pKa = 2.95 VARR77 pKa = 11.84 YY78 pKa = 9.44 RR79 pKa = 11.84 EE80 pKa = 4.66 LINKK84 pKa = 9.78 LGLRR88 pKa = 11.84 RR89 pKa = 3.7
Molecular weight: 10.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.324
IPC2_protein 9.604
IPC_protein 10.511
Toseland 10.774
ProMoST 10.906
Dawson 10.847
Bjellqvist 10.57
Wikipedia 11.082
Rodwell 10.979
Grimsley 10.891
Solomon 11.023
Lehninger 10.979
Nozaki 10.745
DTASelect 10.57
Thurlkill 10.76
EMBOSS 11.184
Sillero 10.789
Patrickios 10.76
IPC_peptide 11.023
IPC2_peptide 9.37
IPC2.peptide.svr19 8.888
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4426
0
4426
1165848
37
2235
263.4
29.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.338 ± 0.044
0.63 ± 0.013
5.517 ± 0.033
8.21 ± 0.051
4.432 ± 0.028
6.933 ± 0.04
2.16 ± 0.019
7.162 ± 0.036
6.526 ± 0.039
9.439 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.965 ± 0.019
4.068 ± 0.03
3.522 ± 0.022
3.744 ± 0.027
4.171 ± 0.033
6.173 ± 0.032
5.486 ± 0.035
7.051 ± 0.03
1.027 ± 0.016
3.448 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here