Sweet potato vein clearing virus
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F2XXY9|F2XXY9_9VIRU Uncharacterized protein OS=Sweet potato vein clearing virus OX=995049 PE=4 SV=1
MM1 pKa = 7.56 CLDD4 pKa = 3.87 KK5 pKa = 11.49 NIVCLNNQQKK15 pKa = 10.94 LDD17 pKa = 3.98 LFNLIMNDD25 pKa = 2.72 FDD27 pKa = 4.55 YY28 pKa = 11.65 VVDD31 pKa = 4.09 NLNNLNTCEE40 pKa = 5.8 LEE42 pKa = 4.31 YY43 pKa = 10.43 FWKK46 pKa = 10.16 IVKK49 pKa = 9.89 KK50 pKa = 10.27 FQLEE54 pKa = 4.11 CLFQRR59 pKa = 11.84 RR60 pKa = 11.84 NVLDD64 pKa = 3.93 DD65 pKa = 3.53
Molecular weight: 7.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.482
IPC2_protein 4.635
IPC_protein 4.431
Toseland 4.228
ProMoST 4.418
Dawson 4.418
Bjellqvist 4.647
Wikipedia 4.355
Rodwell 4.266
Grimsley 4.151
Solomon 4.406
Lehninger 4.355
Nozaki 4.533
DTASelect 4.774
Thurlkill 4.291
EMBOSS 4.368
Sillero 4.546
Patrickios 3.541
IPC_peptide 4.406
IPC2_peptide 4.533
IPC2.peptide.svr19 4.531
Protein with the highest isoelectric point:
>tr|F2XXZ5|F2XXZ5_9VIRU Putative inclusion body protein OS=Sweet potato vein clearing virus OX=995049 PE=4 SV=1
MM1 pKa = 7.51 KK2 pKa = 10.36 LISLLQMSNQDD13 pKa = 2.98 KK14 pKa = 10.8 QMEE17 pKa = 4.45 LMRR20 pKa = 11.84 AMMAQIKK27 pKa = 8.16 ITSEE31 pKa = 3.71 QNAEE35 pKa = 3.93 ILIKK39 pKa = 10.51 LSKK42 pKa = 10.75 LEE44 pKa = 3.95 EE45 pKa = 4.08 RR46 pKa = 11.84 VTTLEE51 pKa = 4.06 SNKK54 pKa = 9.98 EE55 pKa = 4.12 SASQGTDD62 pKa = 2.78 NAPVYY67 pKa = 10.74 NKK69 pKa = 10.75 AKK71 pKa = 10.15 INSKK75 pKa = 10.39 SLVVLVLLRR84 pKa = 11.84 FISKK88 pKa = 10.35 GMDD91 pKa = 3.06 NVGTLRR97 pKa = 11.84 LEE99 pKa = 4.35 KK100 pKa = 10.69 EE101 pKa = 4.06 NSRR104 pKa = 11.84 KK105 pKa = 8.54 TNTRR109 pKa = 3.04
Molecular weight: 12.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.904
IPC2_protein 9.048
IPC_protein 8.99
Toseland 10.175
ProMoST 9.736
Dawson 10.248
Bjellqvist 9.794
Wikipedia 10.321
Rodwell 10.965
Grimsley 10.262
Solomon 10.277
Lehninger 10.277
Nozaki 10.131
DTASelect 9.794
Thurlkill 10.145
EMBOSS 10.54
Sillero 10.16
Patrickios 10.76
IPC_peptide 10.292
IPC2_peptide 8.068
IPC2.peptide.svr19 8.084
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9
0
9
2438
65
650
270.9
31.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.994 ± 0.611
1.764 ± 0.31
5.783 ± 0.302
8.368 ± 0.498
3.733 ± 0.528
3.281 ± 0.411
1.272 ± 0.358
9.352 ± 0.767
11.608 ± 0.925
8.942 ± 0.621
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.215 ± 0.49
7.178 ± 0.722
2.994 ± 0.618
3.404 ± 0.287
4.471 ± 0.459
7.26 ± 0.893
4.307 ± 0.408
5.537 ± 0.705
0.943 ± 0.202
4.594 ± 0.411
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here