Flavobacterium psychrotolerans
Average proteome isoelectric point is 6.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2753 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U1JMW5|A0A2U1JMW5_9FLAO Urease accessory protein UreE OS=Flavobacterium psychrotolerans OX=2169410 GN=ureE PE=3 SV=1
MM1 pKa = 7.51 KK2 pKa = 10.42 KK3 pKa = 8.47 ITLLFMLFIGFSAFAQFPQDD23 pKa = 3.54 FEE25 pKa = 4.86 GTFPPTGWAVYY36 pKa = 10.08 RR37 pKa = 11.84 GANDD41 pKa = 4.43 LGIEE45 pKa = 4.22 SDD47 pKa = 3.4 WTATTVGVATGLQAAYY63 pKa = 10.5 VDD65 pKa = 4.39 YY66 pKa = 10.8 EE67 pKa = 4.13 AVTAGEE73 pKa = 4.26 LAEE76 pKa = 4.79 DD77 pKa = 3.9 WLVSPAYY84 pKa = 9.28 TVTAPNTLMAFYY96 pKa = 10.11 QGQDD100 pKa = 3.12 DD101 pKa = 4.03 PTDD104 pKa = 3.52 YY105 pKa = 11.36 GSTYY109 pKa = 9.27 TIRR112 pKa = 11.84 VSTTSQIDD120 pKa = 3.4 INSFTIVDD128 pKa = 4.01 TQTEE132 pKa = 4.34 SDD134 pKa = 3.75 FTIEE138 pKa = 3.9 MTTRR142 pKa = 11.84 TVDD145 pKa = 3.51 LSAYY149 pKa = 9.63 AGQTIYY155 pKa = 10.61 IAFVLEE161 pKa = 4.15 NNEE164 pKa = 3.58 GDD166 pKa = 3.06 AWAIDD171 pKa = 4.43 GIDD174 pKa = 4.05 FISNTVIAPGCVSNPSPADD193 pKa = 3.12 AALEE197 pKa = 4.21 VPIGATTFSWTAPTTGGPVTLYY219 pKa = 10.64 NIYY222 pKa = 10.73 SGATSDD228 pKa = 4.45 AVTDD232 pKa = 5.27 FITSTTTPSVDD243 pKa = 3.54 LSLADD248 pKa = 4.71 FSTTFYY254 pKa = 10.18 WKK256 pKa = 10.05 VVAVNLVGSATDD268 pKa = 3.46 CAVWSFTTQSPPGYY282 pKa = 9.57 CLAASGGQYY291 pKa = 10.05 PADD294 pKa = 3.97 AYY296 pKa = 9.99 TPEE299 pKa = 4.52 ILDD302 pKa = 3.76 GVTEE306 pKa = 4.41 NIIVSDD312 pKa = 4.19 GFAGEE317 pKa = 4.04 YY318 pKa = 10.45 SVVNVLSGQTYY329 pKa = 7.8 TFTSGTTDD337 pKa = 4.9 FITIGNEE344 pKa = 3.76 AGDD347 pKa = 3.67 TSLIAGASPLTWVSDD362 pKa = 3.13 ISGVIRR368 pKa = 11.84 FYY370 pKa = 11.61 SHH372 pKa = 7.32 VDD374 pKa = 3.48 DD375 pKa = 4.26 QCGEE379 pKa = 4.12 EE380 pKa = 4.32 NIEE383 pKa = 4.07 RR384 pKa = 11.84 PRR386 pKa = 11.84 TVISDD391 pKa = 3.86 PLLATDD397 pKa = 4.73 TFNSASFHH405 pKa = 5.7 TFPNPVKK412 pKa = 10.59 DD413 pKa = 3.88 ILNISYY419 pKa = 10.42 DD420 pKa = 3.76 KK421 pKa = 11.01 NISNVAVFNLLGQEE435 pKa = 4.16 VMTKK439 pKa = 8.87 SVKK442 pKa = 9.96 QNQSQVDD449 pKa = 3.91 MSHH452 pKa = 6.92 LSNGTYY458 pKa = 8.32 MVKK461 pKa = 8.8 VTSDD465 pKa = 3.46 NQVKK469 pKa = 8.88 TIKK472 pKa = 10.31 VIKK475 pKa = 10.09 GG476 pKa = 3.21
Molecular weight: 51.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.76
IPC2_protein 3.795
IPC_protein 3.821
Toseland 3.592
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.757
Rodwell 3.643
Grimsley 3.49
Solomon 3.808
Lehninger 3.77
Nozaki 3.923
DTASelect 4.19
Thurlkill 3.643
EMBOSS 3.757
Sillero 3.935
Patrickios 0.54
IPC_peptide 3.808
IPC2_peptide 3.91
IPC2.peptide.svr19 3.834
Protein with the highest isoelectric point:
>tr|A0A2U1JFJ3|A0A2U1JFJ3_9FLAO IS91 family transposase OS=Flavobacterium psychrotolerans OX=2169410 GN=DB895_14025 PE=4 SV=1
RR1 pKa = 7.43 GAPGVDD7 pKa = 2.48 RR8 pKa = 11.84 LTVEE12 pKa = 4.37 EE13 pKa = 4.4 LKK15 pKa = 10.93 DD16 pKa = 3.23 WLLVHH21 pKa = 6.55 WPSVKK26 pKa = 10.32 AALLEE31 pKa = 4.08 DD32 pKa = 4.47 RR33 pKa = 11.84 YY34 pKa = 10.91 LPRR37 pKa = 11.84 AVRR40 pKa = 11.84 RR41 pKa = 11.84 VDD43 pKa = 3.0 IPKK46 pKa = 9.93 PNGGVRR52 pKa = 11.84 TLGVPTVVDD61 pKa = 3.65 RR62 pKa = 11.84 LIQQALHH69 pKa = 5.99 QVLQPIFEE77 pKa = 4.52 PTFSDD82 pKa = 3.39 GSYY85 pKa = 10.61 GFRR88 pKa = 11.84 PQRR91 pKa = 11.84 SALDD95 pKa = 3.4 AVVKK99 pKa = 10.48 AKK101 pKa = 10.59 EE102 pKa = 3.82 YY103 pKa = 9.61 VAEE106 pKa = 4.68 GKK108 pKa = 9.58 HH109 pKa = 4.47 WVVDD113 pKa = 3.15 IDD115 pKa = 3.99 LEE117 pKa = 4.57 KK118 pKa = 11.04 FFDD121 pKa = 3.98 RR122 pKa = 11.84 VNHH125 pKa = 6.24 DD126 pKa = 3.15 VLMARR131 pKa = 11.84 LAYY134 pKa = 9.58 RR135 pKa = 11.84 VRR137 pKa = 11.84 DD138 pKa = 3.62 GRR140 pKa = 11.84 VLKK143 pKa = 10.51 LIRR146 pKa = 11.84 RR147 pKa = 11.84 FLEE150 pKa = 4.05 AGMMANGVIQPRR162 pKa = 11.84 SEE164 pKa = 3.88 GTPQGGPLSPLLSNILLTDD183 pKa = 4.08 LDD185 pKa = 4.16 RR186 pKa = 11.84 EE187 pKa = 4.33 LEE189 pKa = 3.97 RR190 pKa = 11.84 RR191 pKa = 11.84 QLKK194 pKa = 8.83 FCRR197 pKa = 11.84 YY198 pKa = 10.23 ADD200 pKa = 3.8 DD201 pKa = 4.04 CNIYY205 pKa = 10.13 IGSEE209 pKa = 4.15 GAGQRR214 pKa = 11.84 AMANIRR220 pKa = 11.84 AFLEE224 pKa = 4.02 GHH226 pKa = 6.27 LKK228 pKa = 10.61 LRR230 pKa = 11.84 INEE233 pKa = 4.05 QKK235 pKa = 10.77 SAVARR240 pKa = 11.84 PWKK243 pKa = 10.37 RR244 pKa = 11.84 KK245 pKa = 8.5 FLGYY249 pKa = 10.52 AITIYY254 pKa = 10.3 RR255 pKa = 11.84 QEE257 pKa = 4.05 TRR259 pKa = 11.84 VRR261 pKa = 11.84 PAPEE265 pKa = 3.66 SLRR268 pKa = 11.84 RR269 pKa = 11.84 LMDD272 pKa = 3.49 RR273 pKa = 11.84 VRR275 pKa = 11.84 EE276 pKa = 3.97 LLRR279 pKa = 11.84 KK280 pKa = 9.72 GRR282 pKa = 11.84 GRR284 pKa = 11.84 SLSHH288 pKa = 6.64 TIEE291 pKa = 3.9 MLNPVLRR298 pKa = 11.84 GWANYY303 pKa = 8.69 FRR305 pKa = 11.84 LTANMRR311 pKa = 11.84 MLEE314 pKa = 3.99 EE315 pKa = 4.4 LDD317 pKa = 2.56 WWLRR321 pKa = 11.84 RR322 pKa = 11.84 KK323 pKa = 9.23 LRR325 pKa = 11.84 CLLWRR330 pKa = 11.84 QWKK333 pKa = 7.62 TPKK336 pKa = 9.11 TRR338 pKa = 11.84 EE339 pKa = 3.55 RR340 pKa = 11.84 RR341 pKa = 11.84 LILLGLRR348 pKa = 11.84 PEE350 pKa = 4.41 RR351 pKa = 11.84 AWKK354 pKa = 10.52 SSVNGRR360 pKa = 11.84 GPWWNSASKK369 pKa = 10.6 HH370 pKa = 4.88 MNQAVPTLYY379 pKa = 10.88
Molecular weight: 44.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.343
IPC2_protein 9.677
IPC_protein 10.613
Toseland 10.774
ProMoST 10.643
Dawson 10.847
Bjellqvist 10.599
Wikipedia 11.096
Rodwell 10.979
Grimsley 10.906
Solomon 11.008
Lehninger 10.979
Nozaki 10.745
DTASelect 10.599
Thurlkill 10.774
EMBOSS 11.184
Sillero 10.789
Patrickios 10.672
IPC_peptide 11.023
IPC2_peptide 9.589
IPC2.peptide.svr19 8.714
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2753
0
2753
953119
34
3340
346.2
38.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.433 ± 0.061
0.824 ± 0.016
5.124 ± 0.036
6.068 ± 0.071
5.385 ± 0.044
6.521 ± 0.062
1.689 ± 0.025
8.414 ± 0.044
7.886 ± 0.072
9.129 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.223 ± 0.025
6.398 ± 0.049
3.4 ± 0.027
3.346 ± 0.025
3.061 ± 0.03
6.679 ± 0.052
6.251 ± 0.103
6.254 ± 0.037
1.003 ± 0.018
3.911 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here