Geobacter argillaceus
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3944 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A562VL82|A0A562VL82_9DELT Uncharacterized protein OS=Geobacter argillaceus OX=345631 GN=JN12_02466 PE=4 SV=1
MM1 pKa = 6.8 QQWKK5 pKa = 8.45 CTVCGYY11 pKa = 10.92 VYY13 pKa = 10.87 DD14 pKa = 4.3 PVEE17 pKa = 4.77 GDD19 pKa = 3.73 ADD21 pKa = 3.72 NGVPPGTPFEE31 pKa = 4.19 EE32 pKa = 5.66 LPDD35 pKa = 3.4 GWACPICGAGKK46 pKa = 10.24 DD47 pKa = 3.84 LFEE50 pKa = 5.86 KK51 pKa = 10.48 II52 pKa = 4.12
Molecular weight: 5.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.858
IPC2_protein 3.961
IPC_protein 3.808
Toseland 3.617
ProMoST 3.999
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.77
Rodwell 3.656
Grimsley 3.541
Solomon 3.77
Lehninger 3.732
Nozaki 3.948
DTASelect 4.113
Thurlkill 3.706
EMBOSS 3.77
Sillero 3.935
Patrickios 0.299
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.842
Protein with the highest isoelectric point:
>tr|A0A562VNT2|A0A562VNT2_9DELT DNA-3-methyladenine glycosylase I OS=Geobacter argillaceus OX=345631 GN=JN12_01820 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNTSRR12 pKa = 11.84 KK13 pKa = 7.49 RR14 pKa = 11.84 THH16 pKa = 6.14 GFLVRR21 pKa = 11.84 MATKK25 pKa = 10.45 NGRR28 pKa = 11.84 LVIKK32 pKa = 10.39 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.74 GRR39 pKa = 11.84 KK40 pKa = 8.4 RR41 pKa = 11.84 LAVGIATKK49 pKa = 10.64
Molecular weight: 5.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3944
0
3944
1295148
39
5886
328.4
36.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.752 ± 0.044
1.269 ± 0.024
5.12 ± 0.025
6.344 ± 0.053
3.96 ± 0.022
8.199 ± 0.041
2.017 ± 0.02
5.905 ± 0.031
4.669 ± 0.042
10.382 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.438 ± 0.02
3.256 ± 0.033
4.623 ± 0.031
3.208 ± 0.022
6.368 ± 0.045
5.583 ± 0.034
5.634 ± 0.051
7.475 ± 0.036
1.014 ± 0.015
2.786 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here