Amino acid dipepetide frequency for Ralstonia phage RPZH6

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.799AlaAla: 22.799 ± 1.843
0.931AlaCys: 0.931 ± 0.246
6.152AlaAsp: 6.152 ± 0.559
9.357AlaGlu: 9.357 ± 1.074
3.205AlaPhe: 3.205 ± 0.428
11.115AlaGly: 11.115 ± 1.019
2.74AlaHis: 2.74 ± 0.375
4.705AlaIle: 4.705 ± 0.469
5.428AlaLys: 5.428 ± 0.685
9.461AlaLeu: 9.461 ± 0.654
3.257AlaMet: 3.257 ± 0.487
4.188AlaAsn: 4.188 ± 0.649
8.375AlaPro: 8.375 ± 1.517
5.79AlaGln: 5.79 ± 0.687
8.944AlaArg: 8.944 ± 0.67
7.031AlaSer: 7.031 ± 0.773
6.359AlaThr: 6.359 ± 0.514
7.341AlaVal: 7.341 ± 0.574
2.016AlaTrp: 2.016 ± 0.39
3.722AlaTyr: 3.722 ± 0.438
0.0AlaXaa: 0.0 ± 0.0
Cys
0.517CysAla: 0.517 ± 0.17
0.207CysCys: 0.207 ± 0.108
0.517CysAsp: 0.517 ± 0.217
0.414CysGlu: 0.414 ± 0.283
0.31CysPhe: 0.31 ± 0.153
0.724CysGly: 0.724 ± 0.2
0.362CysHis: 0.362 ± 0.178
0.465CysIle: 0.465 ± 0.175
0.362CysLys: 0.362 ± 0.15
0.517CysLeu: 0.517 ± 0.173
0.207CysMet: 0.207 ± 0.117
0.207CysAsn: 0.207 ± 0.101
0.258CysPro: 0.258 ± 0.115
0.103CysGln: 0.103 ± 0.079
0.827CysArg: 0.827 ± 0.249
0.362CysSer: 0.362 ± 0.141
0.414CysThr: 0.414 ± 0.145
0.569CysVal: 0.569 ± 0.208
0.258CysTrp: 0.258 ± 0.118
0.258CysTyr: 0.258 ± 0.133
0.0CysXaa: 0.0 ± 0.0
Asp
8.479AspAla: 8.479 ± 0.7
0.258AspCys: 0.258 ± 0.13
4.084AspAsp: 4.084 ± 0.607
4.549AspGlu: 4.549 ± 0.447
1.861AspPhe: 1.861 ± 0.286
7.755AspGly: 7.755 ± 0.689
1.448AspHis: 1.448 ± 0.328
2.999AspIle: 2.999 ± 0.429
1.809AspLys: 1.809 ± 0.296
4.601AspLeu: 4.601 ± 0.435
1.241AspMet: 1.241 ± 0.282
1.809AspAsn: 1.809 ± 0.262
2.326AspPro: 2.326 ± 0.369
1.706AspGln: 1.706 ± 0.445
4.343AspArg: 4.343 ± 0.504
2.637AspSer: 2.637 ± 0.393
3.257AspThr: 3.257 ± 0.394
3.464AspVal: 3.464 ± 0.459
1.034AspTrp: 1.034 ± 0.206
1.758AspTyr: 1.758 ± 0.315
0.0AspXaa: 0.0 ± 0.0
Glu
7.858GluAla: 7.858 ± 0.864
0.465GluCys: 0.465 ± 0.148
3.722GluAsp: 3.722 ± 0.461
3.774GluGlu: 3.774 ± 0.517
2.482GluPhe: 2.482 ± 0.39
4.032GluGly: 4.032 ± 0.601
1.551GluHis: 1.551 ± 0.293
3.464GluIle: 3.464 ± 0.404
2.895GluLys: 2.895 ± 0.429
5.325GluLeu: 5.325 ± 0.517
1.861GluMet: 1.861 ± 0.365
2.895GluAsn: 2.895 ± 0.354
3.309GluPro: 3.309 ± 0.504
3.877GluGln: 3.877 ± 0.441
6.255GluArg: 6.255 ± 0.696
3.05GluSer: 3.05 ± 0.326
2.326GluThr: 2.326 ± 0.393
2.585GluVal: 2.585 ± 0.329
0.724GluTrp: 0.724 ± 0.198
1.448GluTyr: 1.448 ± 0.267
0.0GluXaa: 0.0 ± 0.0
Phe
2.947PheAla: 2.947 ± 0.352
0.465PheCys: 0.465 ± 0.176
2.533PheAsp: 2.533 ± 0.387
1.965PheGlu: 1.965 ± 0.298
0.775PhePhe: 0.775 ± 0.28
2.378PheGly: 2.378 ± 0.303
0.414PheHis: 0.414 ± 0.122
1.654PheIle: 1.654 ± 0.346
0.879PheLys: 0.879 ± 0.201
1.809PheLeu: 1.809 ± 0.329
0.931PheMet: 0.931 ± 0.241
1.034PheAsn: 1.034 ± 0.217
1.034PhePro: 1.034 ± 0.239
1.034PheGln: 1.034 ± 0.258
1.654PheArg: 1.654 ± 0.422
1.344PheSer: 1.344 ± 0.286
1.086PheThr: 1.086 ± 0.239
2.637PheVal: 2.637 ± 0.362
0.517PheTrp: 0.517 ± 0.149
1.189PheTyr: 1.189 ± 0.271
0.0PheXaa: 0.0 ± 0.0
Gly
11.27GlyAla: 11.27 ± 1.25
0.775GlyCys: 0.775 ± 0.201
5.842GlyAsp: 5.842 ± 0.527
4.963GlyGlu: 4.963 ± 0.664
1.913GlyPhe: 1.913 ± 0.298
9.668GlyGly: 9.668 ± 1.124
2.378GlyHis: 2.378 ± 0.357
3.515GlyIle: 3.515 ± 0.53
4.808GlyLys: 4.808 ± 0.509
4.498GlyLeu: 4.498 ± 0.492
2.12GlyMet: 2.12 ± 0.309
2.895GlyAsn: 2.895 ± 0.469
2.482GlyPro: 2.482 ± 0.27
3.205GlyGln: 3.205 ± 0.481
6.049GlyArg: 6.049 ± 0.707
4.549GlySer: 4.549 ± 0.588
6.1GlyThr: 6.1 ± 0.632
5.428GlyVal: 5.428 ± 0.563
1.241GlyTrp: 1.241 ± 0.21
2.326GlyTyr: 2.326 ± 0.372
0.0GlyXaa: 0.0 ± 0.0
His
2.482HisAla: 2.482 ± 0.38
0.362HisCys: 0.362 ± 0.142
1.603HisAsp: 1.603 ± 0.303
2.016HisGlu: 2.016 ± 0.309
1.034HisPhe: 1.034 ± 0.197
1.499HisGly: 1.499 ± 0.329
0.724HisHis: 0.724 ± 0.185
1.137HisIle: 1.137 ± 0.203
0.517HisLys: 0.517 ± 0.16
1.396HisLeu: 1.396 ± 0.3
0.672HisMet: 0.672 ± 0.178
0.62HisAsn: 0.62 ± 0.207
1.086HisPro: 1.086 ± 0.207
0.62HisGln: 0.62 ± 0.168
1.603HisArg: 1.603 ± 0.304
1.137HisSer: 1.137 ± 0.266
1.137HisThr: 1.137 ± 0.21
1.189HisVal: 1.189 ± 0.271
0.052HisTrp: 0.052 ± 0.057
0.414HisTyr: 0.414 ± 0.162
0.0HisXaa: 0.0 ± 0.0
Ile
5.48IleAla: 5.48 ± 0.589
0.155IleCys: 0.155 ± 0.089
3.205IleAsp: 3.205 ± 0.362
4.239IleGlu: 4.239 ± 0.644
1.034IlePhe: 1.034 ± 0.224
3.929IleGly: 3.929 ± 0.487
0.931IleHis: 0.931 ± 0.242
1.913IleIle: 1.913 ± 0.337
2.12IleLys: 2.12 ± 0.32
2.378IleLeu: 2.378 ± 0.368
1.551IleMet: 1.551 ± 0.292
2.171IleAsn: 2.171 ± 0.38
2.016IlePro: 2.016 ± 0.295
2.171IleGln: 2.171 ± 0.382
2.947IleArg: 2.947 ± 0.522
3.102IleSer: 3.102 ± 0.448
2.275IleThr: 2.275 ± 0.364
3.981IleVal: 3.981 ± 0.5
0.258IleTrp: 0.258 ± 0.116
1.034IleTyr: 1.034 ± 0.232
0.0IleXaa: 0.0 ± 0.0
Lys
5.997LysAla: 5.997 ± 0.662
0.155LysCys: 0.155 ± 0.089
2.275LysAsp: 2.275 ± 0.359
2.637LysGlu: 2.637 ± 0.436
1.034LysPhe: 1.034 ± 0.285
3.154LysGly: 3.154 ± 0.484
0.672LysHis: 0.672 ± 0.159
1.965LysIle: 1.965 ± 0.322
1.654LysLys: 1.654 ± 0.292
3.309LysLeu: 3.309 ± 0.435
1.086LysMet: 1.086 ± 0.185
1.551LysAsn: 1.551 ± 0.328
2.223LysPro: 2.223 ± 0.311
2.223LysGln: 2.223 ± 0.351
4.394LysArg: 4.394 ± 0.495
2.482LysSer: 2.482 ± 0.483
2.585LysThr: 2.585 ± 0.363
2.947LysVal: 2.947 ± 0.504
0.931LysTrp: 0.931 ± 0.231
0.569LysTyr: 0.569 ± 0.14
0.0LysXaa: 0.0 ± 0.0
Leu
7.289LeuAla: 7.289 ± 0.554
0.517LeuCys: 0.517 ± 0.197
5.894LeuAsp: 5.894 ± 0.582
3.722LeuGlu: 3.722 ± 0.49
2.068LeuPhe: 2.068 ± 0.32
6.462LeuGly: 6.462 ± 0.579
1.603LeuHis: 1.603 ± 0.244
3.102LeuIle: 3.102 ± 0.406
3.929LeuLys: 3.929 ± 0.736
4.705LeuLeu: 4.705 ± 0.701
1.396LeuMet: 1.396 ± 0.332
2.12LeuAsn: 2.12 ± 0.323
3.671LeuPro: 3.671 ± 0.703
2.326LeuGln: 2.326 ± 0.333
5.842LeuArg: 5.842 ± 0.582
4.653LeuSer: 4.653 ± 0.57
4.653LeuThr: 4.653 ± 0.419
3.877LeuVal: 3.877 ± 0.514
0.672LeuTrp: 0.672 ± 0.21
1.706LeuTyr: 1.706 ± 0.271
0.0LeuXaa: 0.0 ± 0.0
Met
2.637MetAla: 2.637 ± 0.422
0.052MetCys: 0.052 ± 0.056
1.189MetAsp: 1.189 ± 0.254
0.879MetGlu: 0.879 ± 0.215
0.724MetPhe: 0.724 ± 0.258
2.223MetGly: 2.223 ± 0.369
0.465MetHis: 0.465 ± 0.171
0.931MetIle: 0.931 ± 0.181
1.809MetLys: 1.809 ± 0.303
1.758MetLeu: 1.758 ± 0.318
0.775MetMet: 0.775 ± 0.206
0.827MetAsn: 0.827 ± 0.266
2.016MetPro: 2.016 ± 0.297
1.396MetGln: 1.396 ± 0.281
2.378MetArg: 2.378 ± 0.373
1.809MetSer: 1.809 ± 0.355
1.809MetThr: 1.809 ± 0.291
1.189MetVal: 1.189 ± 0.365
0.258MetTrp: 0.258 ± 0.123
0.569MetTyr: 0.569 ± 0.154
0.0MetXaa: 0.0 ± 0.0
Asn
4.239AsnAla: 4.239 ± 0.544
0.052AsnCys: 0.052 ± 0.05
2.016AsnAsp: 2.016 ± 0.325
1.551AsnGlu: 1.551 ± 0.235
0.879AsnPhe: 0.879 ± 0.245
4.136AsnGly: 4.136 ± 0.675
0.672AsnHis: 0.672 ± 0.215
1.396AsnIle: 1.396 ± 0.326
1.241AsnLys: 1.241 ± 0.293
2.378AsnLeu: 2.378 ± 0.308
0.982AsnMet: 0.982 ± 0.228
1.086AsnAsn: 1.086 ± 0.259
2.326AsnPro: 2.326 ± 0.46
1.086AsnGln: 1.086 ± 0.257
1.965AsnArg: 1.965 ± 0.296
1.861AsnSer: 1.861 ± 0.296
2.585AsnThr: 2.585 ± 0.411
2.275AsnVal: 2.275 ± 0.38
0.672AsnTrp: 0.672 ± 0.171
0.62AsnTyr: 0.62 ± 0.207
0.0AsnXaa: 0.0 ± 0.0
Pro
9.151ProAla: 9.151 ± 1.652
0.414ProCys: 0.414 ± 0.158
2.74ProAsp: 2.74 ± 0.292
3.567ProGlu: 3.567 ± 0.454
1.137ProPhe: 1.137 ± 0.273
4.084ProGly: 4.084 ± 0.439
0.931ProHis: 0.931 ± 0.254
2.688ProIle: 2.688 ± 0.405
1.861ProLys: 1.861 ± 0.294
2.895ProLeu: 2.895 ± 0.412
0.982ProMet: 0.982 ± 0.264
0.982ProAsn: 0.982 ± 0.231
2.12ProPro: 2.12 ± 0.41
1.654ProGln: 1.654 ± 0.351
3.722ProArg: 3.722 ± 0.53
3.36ProSer: 3.36 ± 0.458
3.36ProThr: 3.36 ± 0.387
2.895ProVal: 2.895 ± 0.389
0.362ProTrp: 0.362 ± 0.143
1.189ProTyr: 1.189 ± 0.216
0.0ProXaa: 0.0 ± 0.0
Gln
7.031GlnAla: 7.031 ± 0.887
0.155GlnCys: 0.155 ± 0.111
1.965GlnAsp: 1.965 ± 0.357
2.533GlnGlu: 2.533 ± 0.41
0.879GlnPhe: 0.879 ± 0.168
2.74GlnGly: 2.74 ± 0.379
0.931GlnHis: 0.931 ± 0.245
1.499GlnIle: 1.499 ± 0.389
1.448GlnLys: 1.448 ± 0.289
3.309GlnLeu: 3.309 ± 0.406
1.448GlnMet: 1.448 ± 0.363
1.396GlnAsn: 1.396 ± 0.267
2.068GlnPro: 2.068 ± 0.289
3.826GlnGln: 3.826 ± 0.743
2.585GlnArg: 2.585 ± 0.489
2.171GlnSer: 2.171 ± 0.345
1.758GlnThr: 1.758 ± 0.382
2.482GlnVal: 2.482 ± 0.386
0.775GlnTrp: 0.775 ± 0.162
0.982GlnTyr: 0.982 ± 0.26
0.0GlnXaa: 0.0 ± 0.0
Arg
9.151ArgAla: 9.151 ± 0.83
0.724ArgCys: 0.724 ± 0.208
4.756ArgAsp: 4.756 ± 0.502
4.653ArgGlu: 4.653 ± 0.662
2.843ArgPhe: 2.843 ± 0.456
4.498ArgGly: 4.498 ± 0.443
1.654ArgHis: 1.654 ± 0.392
4.239ArgIle: 4.239 ± 0.536
3.877ArgLys: 3.877 ± 0.499
5.997ArgLeu: 5.997 ± 0.647
2.068ArgMet: 2.068 ± 0.332
2.585ArgAsn: 2.585 ± 0.325
3.102ArgPro: 3.102 ± 0.452
3.05ArgGln: 3.05 ± 0.487
5.17ArgArg: 5.17 ± 0.811
3.722ArgSer: 3.722 ± 0.435
2.999ArgThr: 2.999 ± 0.314
4.446ArgVal: 4.446 ± 0.573
0.982ArgTrp: 0.982 ± 0.202
2.637ArgTyr: 2.637 ± 0.312
0.0ArgXaa: 0.0 ± 0.0
Ser
7.496SerAla: 7.496 ± 0.995
0.517SerCys: 0.517 ± 0.19
3.515SerAsp: 3.515 ± 0.336
3.36SerGlu: 3.36 ± 0.406
1.448SerPhe: 1.448 ± 0.268
5.222SerGly: 5.222 ± 0.703
1.344SerHis: 1.344 ± 0.249
3.102SerIle: 3.102 ± 0.357
2.843SerLys: 2.843 ± 0.347
3.567SerLeu: 3.567 ± 0.401
1.241SerMet: 1.241 ± 0.267
1.965SerAsn: 1.965 ± 0.382
2.895SerPro: 2.895 ± 0.424
2.326SerGln: 2.326 ± 0.387
3.36SerArg: 3.36 ± 0.444
2.895SerSer: 2.895 ± 0.306
3.412SerThr: 3.412 ± 0.423
3.671SerVal: 3.671 ± 0.487
0.982SerTrp: 0.982 ± 0.201
1.241SerTyr: 1.241 ± 0.277
0.0SerXaa: 0.0 ± 0.0
Thr
6.566ThrAla: 6.566 ± 0.518
0.155ThrCys: 0.155 ± 0.102
2.533ThrAsp: 2.533 ± 0.451
3.671ThrGlu: 3.671 ± 0.438
1.913ThrPhe: 1.913 ± 0.285
5.48ThrGly: 5.48 ± 0.673
1.086ThrHis: 1.086 ± 0.221
3.257ThrIle: 3.257 ± 0.487
2.275ThrLys: 2.275 ± 0.329
4.653ThrLeu: 4.653 ± 0.453
1.499ThrMet: 1.499 ± 0.264
1.551ThrAsn: 1.551 ± 0.386
3.36ThrPro: 3.36 ± 0.369
2.12ThrGln: 2.12 ± 0.355
2.792ThrArg: 2.792 ± 0.446
3.257ThrSer: 3.257 ± 0.488
2.843ThrThr: 2.843 ± 0.447
4.394ThrVal: 4.394 ± 0.511
0.362ThrTrp: 0.362 ± 0.134
1.654ThrTyr: 1.654 ± 0.297
0.0ThrXaa: 0.0 ± 0.0
Val
7.445ValAla: 7.445 ± 0.554
0.672ValCys: 0.672 ± 0.227
3.671ValAsp: 3.671 ± 0.447
3.981ValGlu: 3.981 ± 0.564
1.396ValPhe: 1.396 ± 0.266
4.032ValGly: 4.032 ± 0.55
0.775ValHis: 0.775 ± 0.222
2.895ValIle: 2.895 ± 0.478
2.843ValLys: 2.843 ± 0.529
3.722ValLeu: 3.722 ± 0.371
1.654ValMet: 1.654 ± 0.253
2.43ValAsn: 2.43 ± 0.376
4.343ValPro: 4.343 ± 0.407
2.068ValGln: 2.068 ± 0.334
4.653ValArg: 4.653 ± 0.576
4.601ValSer: 4.601 ± 0.418
4.084ValThr: 4.084 ± 0.38
4.084ValVal: 4.084 ± 0.54
0.724ValTrp: 0.724 ± 0.203
1.654ValTyr: 1.654 ± 0.27
0.0ValXaa: 0.0 ± 0.0
Trp
1.086TrpAla: 1.086 ± 0.213
0.31TrpCys: 0.31 ± 0.127
0.827TrpAsp: 0.827 ± 0.239
0.517TrpGlu: 0.517 ± 0.164
0.465TrpPhe: 0.465 ± 0.196
0.775TrpGly: 0.775 ± 0.163
0.258TrpHis: 0.258 ± 0.121
0.724TrpIle: 0.724 ± 0.191
0.465TrpLys: 0.465 ± 0.173
1.551TrpLeu: 1.551 ± 0.295
0.362TrpMet: 0.362 ± 0.127
0.672TrpAsn: 0.672 ± 0.198
0.207TrpPro: 0.207 ± 0.104
0.517TrpGln: 0.517 ± 0.161
1.396TrpArg: 1.396 ± 0.295
0.931TrpSer: 0.931 ± 0.264
0.982TrpThr: 0.982 ± 0.188
0.775TrpVal: 0.775 ± 0.164
0.155TrpTrp: 0.155 ± 0.08
0.258TrpTyr: 0.258 ± 0.112
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.999TyrAla: 2.999 ± 0.394
0.517TyrCys: 0.517 ± 0.161
2.378TyrAsp: 2.378 ± 0.327
1.551TyrGlu: 1.551 ± 0.255
0.931TyrPhe: 0.931 ± 0.257
2.12TyrGly: 2.12 ± 0.418
0.362TyrHis: 0.362 ± 0.137
1.241TyrIle: 1.241 ± 0.257
0.775TyrLys: 0.775 ± 0.167
2.378TyrLeu: 2.378 ± 0.41
0.31TyrMet: 0.31 ± 0.109
1.034TyrAsn: 1.034 ± 0.235
0.879TyrPro: 0.879 ± 0.219
0.879TyrGln: 0.879 ± 0.203
2.275TyrArg: 2.275 ± 0.354
1.448TyrSer: 1.448 ± 0.263
1.396TyrThr: 1.396 ± 0.24
1.499TyrVal: 1.499 ± 0.296
0.258TyrTrp: 0.258 ± 0.183
0.414TyrTyr: 0.414 ± 0.142
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 90 proteins (19344 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski