Flavobacterium phage vB_FspP_elemoA_1-9B

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae; crAss-like viruses

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 89 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D7IYX7|A0A7D7IYX7_9CAUD Uncharacterized protein OS=Flavobacterium phage vB_FspP_elemoA_1-9B OX=2743784 GN=elemo19B_phanotate61 PE=4 SV=1
MM1 pKa = 7.26NVVKK5 pKa = 10.64INTTAYY11 pKa = 9.87EE12 pKa = 4.05EE13 pKa = 4.14EE14 pKa = 4.76DD15 pKa = 4.29FYY17 pKa = 11.96LMTDD21 pKa = 3.73LSEE24 pKa = 5.16DD25 pKa = 3.52DD26 pKa = 4.24LYY28 pKa = 11.43EE29 pKa = 4.44VIMPIVNQEE38 pKa = 3.69RR39 pKa = 11.84YY40 pKa = 10.33GYY42 pKa = 10.29EE43 pKa = 4.3DD44 pKa = 3.96YY45 pKa = 11.48DD46 pKa = 4.21NEE48 pKa = 4.57SLLLALKK55 pKa = 10.23KK56 pKa = 10.26RR57 pKa = 11.84YY58 pKa = 6.91PTNKK62 pKa = 9.04VDD64 pKa = 3.7IIEE67 pKa = 4.27IEE69 pKa = 4.33EE70 pKa = 3.65ISYY73 pKa = 11.16

Molecular weight: 8.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D7IYW8|A0A7D7IYW8_9CAUD Uncharacterized protein OS=Flavobacterium phage vB_FspP_elemoA_1-9B OX=2743784 GN=elemo19B_phanotate41 PE=4 SV=1
MM1 pKa = 6.93QRR3 pKa = 11.84CLSLSVFGSIRR14 pKa = 11.84NKK16 pKa = 10.73LNGSSFSLKK25 pKa = 9.63IVRR28 pKa = 11.84IAQLVRR34 pKa = 11.84AIDD37 pKa = 3.48SS38 pKa = 3.57

Molecular weight: 4.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

89

0

89

19112

30

3667

214.7

24.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.337 ± 0.461

0.717 ± 0.171

6.901 ± 0.214

7.383 ± 0.291

4.552 ± 0.223

5.918 ± 0.245

1.172 ± 0.205

7.718 ± 0.324

9.293 ± 0.619

7.843 ± 0.263

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.281 ± 0.173

7.215 ± 0.19

2.716 ± 0.132

3.05 ± 0.264

3.495 ± 0.157

7.194 ± 0.216

5.667 ± 0.323

6.059 ± 0.232

0.884 ± 0.123

4.604 ± 0.302
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski