Staphylococcus virus IPLA35

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Triavirus

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B7T0K9|B7T0K9_9CAUD Gp58 OS=Staphylococcus virus IPLA35 OX=575610 PE=4 SV=1
MM1 pKa = 7.66LKK3 pKa = 10.44LISPTFEE10 pKa = 6.11DD11 pKa = 2.99IKK13 pKa = 10.15TWYY16 pKa = 8.82QLKK19 pKa = 10.0EE20 pKa = 3.87YY21 pKa = 10.27SKK23 pKa = 11.37EE24 pKa = 4.08DD25 pKa = 3.27IAWYY29 pKa = 10.85VDD31 pKa = 3.16MEE33 pKa = 4.57VIDD36 pKa = 3.95KK37 pKa = 10.8EE38 pKa = 4.28EE39 pKa = 3.79YY40 pKa = 10.74AIITGEE46 pKa = 4.12KK47 pKa = 9.76YY48 pKa = 10.36PEE50 pKa = 4.08NLEE53 pKa = 4.23SS54 pKa = 3.57

Molecular weight: 6.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B7T0H3|B7T0H3_9CAUD Gp22 OS=Staphylococcus virus IPLA35 OX=575610 PE=4 SV=1
MM1 pKa = 7.3ATNTLEE7 pKa = 4.17LSSTINQRR15 pKa = 11.84YY16 pKa = 9.51KK17 pKa = 10.74YY18 pKa = 8.6DD19 pKa = 3.76TAGKK23 pKa = 8.11TPTQIQSEE31 pKa = 4.3LRR33 pKa = 11.84KK34 pKa = 10.05KK35 pKa = 9.95GLQGFVVKK43 pKa = 10.76VSGRR47 pKa = 11.84KK48 pKa = 6.58VTMKK52 pKa = 10.66VMEE55 pKa = 3.89QHH57 pKa = 6.01IKK59 pKa = 10.52SNRR62 pKa = 11.84GCMRR66 pKa = 4.15

Molecular weight: 7.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

13889

50

2066

224.0

25.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.918 ± 0.596

0.475 ± 0.1

6.084 ± 0.26

7.74 ± 0.422

3.946 ± 0.245

5.962 ± 0.559

1.735 ± 0.138

7.042 ± 0.36

9.886 ± 0.473

8.374 ± 0.261

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.556 ± 0.125

6.365 ± 0.273

2.714 ± 0.198

3.722 ± 0.147

4.126 ± 0.208

6.365 ± 0.317

5.753 ± 0.277

5.623 ± 0.224

1.267 ± 0.13

4.349 ± 0.355
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski