Candidatus Hepatoplasma crinochetorum Av
Average proteome isoelectric point is 7.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 582 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W8GMW0|W8GMW0_9MOLU Isoleucine--tRNA ligase OS=Candidatus Hepatoplasma crinochetorum Av OX=1427984 GN=ileS PE=3 SV=1
MM1 pKa = 7.97 IDD3 pKa = 3.27 GCNGGNIDD11 pKa = 4.07 ILSINTINYY20 pKa = 8.81 NSEE23 pKa = 3.91 GEE25 pKa = 4.25 KK26 pKa = 8.61 TQKK29 pKa = 9.98 EE30 pKa = 4.26 VNEE33 pKa = 4.37 TNKK36 pKa = 10.38 EE37 pKa = 4.14 AIDD40 pKa = 4.06 DD41 pKa = 4.85 LINNNMVDD49 pKa = 4.05 TDD51 pKa = 3.8 HH52 pKa = 6.19 VTYY55 pKa = 9.91 TVLGNTSTEE64 pKa = 3.81 AFGDD68 pKa = 4.05 FTQADD73 pKa = 3.74 EE74 pKa = 4.3 KK75 pKa = 11.41 LYY77 pKa = 11.33 GEE79 pKa = 4.48 STNDD83 pKa = 3.09 TVVRR87 pKa = 11.84 DD88 pKa = 3.81 NTLLGLTDD96 pKa = 4.48 GLQDD100 pKa = 4.95 QIDD103 pKa = 4.19 GLNTAVNNLGDD114 pKa = 4.57 IINNAIGDD122 pKa = 4.01 IKK124 pKa = 11.16 ASLDD128 pKa = 3.64 SLSTCKK134 pKa = 10.5 DD135 pKa = 3.35 CSIDD139 pKa = 3.76 PFPSAIIDD147 pKa = 3.97 NIVPTDD153 pKa = 3.59 PTTEE157 pKa = 4.45 DD158 pKa = 3.44 GNGTVSFDD166 pKa = 4.65 VYY168 pKa = 11.26 LSDD171 pKa = 5.45 CNGTSIAVLNPASGTEE187 pKa = 4.32 PITLSEE193 pKa = 4.71 GINTGLTFTDD203 pKa = 5.1 LPPGYY208 pKa = 10.54 YY209 pKa = 10.17 NIEE212 pKa = 4.32 VYY214 pKa = 10.73 CDD216 pKa = 3.26 TEE218 pKa = 5.84 LIDD221 pKa = 3.54 NTSFIINDD229 pKa = 3.52 YY230 pKa = 11.06 DD231 pKa = 3.92 SSEE234 pKa = 4.51 DD235 pKa = 3.44 GGGDD239 pKa = 3.54 EE240 pKa = 5.03 EE241 pKa = 6.1 IIDD244 pKa = 4.46 IVTPDD249 pKa = 4.78 DD250 pKa = 4.27 GSLIPPNSNDD260 pKa = 2.89 IPVNDD265 pKa = 4.51 GEE267 pKa = 4.66 DD268 pKa = 3.64 FNHH271 pKa = 6.84 ILMITKK277 pKa = 10.4 GNLIPEE283 pKa = 4.35 NNIQCYY289 pKa = 9.59 QNLFIVGSDD298 pKa = 3.46 FNLNEE303 pKa = 3.95 WTTGCLNSFNPNDD316 pKa = 3.9 PQGDD320 pKa = 3.59 EE321 pKa = 4.73 CYY323 pKa = 10.68 VAIKK327 pKa = 9.35 RR328 pKa = 11.84 TSDD331 pKa = 3.03 NTFDD335 pKa = 3.54 TTRR338 pKa = 11.84 EE339 pKa = 4.16 SVSNSVKK346 pKa = 10.02 GLNWIKK352 pKa = 10.78 AIDD355 pKa = 4.41 NITITKK361 pKa = 9.68 ILEE364 pKa = 4.24 NGAFGYY370 pKa = 10.7 GDD372 pKa = 3.48 KK373 pKa = 10.83 FVLNEE378 pKa = 4.62 DD379 pKa = 3.56 YY380 pKa = 11.18 TNYY383 pKa = 11.11 DD384 pKa = 3.94 LIISKK389 pKa = 9.54 WYY391 pKa = 9.93 FNTSAGTAVKK401 pKa = 9.12 PQYY404 pKa = 10.29 GYY406 pKa = 9.78 PIRR409 pKa = 11.84 EE410 pKa = 3.91 ISDD413 pKa = 4.77 LIVTNTWLQKK423 pKa = 8.94 QHH425 pKa = 5.66 GSNVSYY431 pKa = 10.7 SGEE434 pKa = 3.97 LTFTDD439 pKa = 4.02 TLNGTVTKK447 pKa = 10.61 ARR449 pKa = 11.84 EE450 pKa = 4.04 SDD452 pKa = 3.99 FLATMWGVNGIPAPIDD468 pKa = 3.42 LNAQVSGTIINMDD481 pKa = 4.28 DD482 pKa = 3.55 YY483 pKa = 12.02 SNADD487 pKa = 3.55 FLLINCTYY495 pKa = 11.37 ADD497 pKa = 3.47 IGNTQLMYY505 pKa = 11.0 AKK507 pKa = 10.3 DD508 pKa = 3.31 IVYY511 pKa = 10.45 DD512 pKa = 3.32 GTTRR516 pKa = 11.84 YY517 pKa = 10.84 DD518 pKa = 3.33 FVGIGACYY526 pKa = 10.12 FSFNVDD532 pKa = 2.88 KK533 pKa = 10.53 TINIIDD539 pKa = 3.93 AALSAEE545 pKa = 4.45 IIITSIKK552 pKa = 10.36 KK553 pKa = 10.22 IILL556 pKa = 3.78
Molecular weight: 60.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.718
IPC2_protein 3.681
IPC_protein 3.732
Toseland 3.503
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.681
Rodwell 3.554
Grimsley 3.401
Solomon 3.732
Lehninger 3.681
Nozaki 3.834
DTASelect 4.113
Thurlkill 3.554
EMBOSS 3.681
Sillero 3.859
Patrickios 0.693
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.757
Protein with the highest isoelectric point:
>tr|W8GEA7|W8GEA7_9MOLU Single-stranded DNA-binding protein OS=Candidatus Hepatoplasma crinochetorum Av OX=1427984 GN=ssb PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.38 QPNKK9 pKa = 8.29 RR10 pKa = 11.84 KK11 pKa = 9.61 KK12 pKa = 10.05 AKK14 pKa = 8.31 THH16 pKa = 5.38 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 KK22 pKa = 8.03 ATKK25 pKa = 9.69 QGQKK29 pKa = 8.5 VVKK32 pKa = 9.76 SRR34 pKa = 11.84 RR35 pKa = 11.84 SKK37 pKa = 10.68 GRR39 pKa = 11.84 KK40 pKa = 9.07 KK41 pKa = 9.85 LTNN44 pKa = 3.53
Molecular weight: 5.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.336
IPC2_protein 10.994
IPC_protein 12.252
Toseland 12.442
ProMoST 12.896
Dawson 12.442
Bjellqvist 12.413
Wikipedia 12.896
Rodwell 12.427
Grimsley 12.486
Solomon 12.91
Lehninger 12.822
Nozaki 12.427
DTASelect 12.413
Thurlkill 12.427
EMBOSS 12.925
Sillero 12.427
Patrickios 12.149
IPC_peptide 12.91
IPC2_peptide 11.886
IPC2.peptide.svr19 8.9
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
582
0
582
201572
30
1935
346.3
40.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.943 ± 0.094
0.374 ± 0.022
5.795 ± 0.102
6.576 ± 0.091
6.074 ± 0.127
4.733 ± 0.114
1.106 ± 0.032
11.724 ± 0.106
11.373 ± 0.214
9.678 ± 0.107
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.419 ± 0.037
9.137 ± 0.14
2.264 ± 0.041
3.029 ± 0.059
2.531 ± 0.075
5.91 ± 0.079
4.496 ± 0.115
4.118 ± 0.078
0.968 ± 0.041
4.752 ± 0.096
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here