Lactobacillus algidus DSM 15638
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1505 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R1HRH6|A0A0R1HRH6_9LACO Arb protein OS=Lactobacillus algidus DSM 15638 OX=1423719 GN=FC66_GL001016 PE=3 SV=1
MM1 pKa = 7.47 YY2 pKa = 9.28 IYY4 pKa = 10.55 SYY6 pKa = 10.57 IIPLLNGLYY15 pKa = 10.18 EE16 pKa = 4.28 SEE18 pKa = 4.53 EE19 pKa = 4.15 PDD21 pKa = 3.17 FTEE24 pKa = 4.79 QLNSYY29 pKa = 10.48 SKK31 pKa = 11.11 DD32 pKa = 3.26 LAKK35 pKa = 10.56 EE36 pKa = 4.03 IILDD40 pKa = 4.46 LIGATGIDD48 pKa = 3.01 IQEE51 pKa = 4.05 FDD53 pKa = 4.05 KK54 pKa = 11.49 LAMKK58 pKa = 10.23 LL59 pKa = 3.55
Molecular weight: 6.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.822
IPC2_protein 4.075
IPC_protein 3.923
Toseland 3.745
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.821
Rodwell 3.757
Grimsley 3.656
Solomon 3.872
Lehninger 3.821
Nozaki 4.024
DTASelect 4.164
Thurlkill 3.795
EMBOSS 3.821
Sillero 4.037
Patrickios 1.914
IPC_peptide 3.872
IPC2_peptide 4.012
IPC2.peptide.svr19 3.939
Protein with the highest isoelectric point:
>tr|A0A0R1HHT3|A0A0R1HHT3_9LACO Uracil-DNA glycosylase OS=Lactobacillus algidus DSM 15638 OX=1423719 GN=ung PE=3 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.69 QPKK8 pKa = 8.34 KK9 pKa = 7.47 RR10 pKa = 11.84 HH11 pKa = 5.71 RR12 pKa = 11.84 EE13 pKa = 3.7 RR14 pKa = 11.84 VHH16 pKa = 6.23 GFRR19 pKa = 11.84 KK20 pKa = 10.04 RR21 pKa = 11.84 MSTKK25 pKa = 9.99 GGRR28 pKa = 11.84 NVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 9.05 GRR39 pKa = 11.84 KK40 pKa = 8.7 VLSAA44 pKa = 4.05
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.356
IPC2_protein 10.906
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.486
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.223
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 11.959
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.069
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1505
0
1505
467948
44
2127
310.9
34.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.153 ± 0.068
0.374 ± 0.014
5.876 ± 0.058
6.209 ± 0.07
4.427 ± 0.052
6.686 ± 0.059
1.736 ± 0.031
8.009 ± 0.066
7.15 ± 0.062
9.555 ± 0.077
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.876 ± 0.029
5.14 ± 0.051
3.281 ± 0.032
3.861 ± 0.044
3.54 ± 0.049
6.471 ± 0.06
6.238 ± 0.06
7.136 ± 0.054
0.876 ± 0.022
3.406 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here