Invertebrate iridescent virus 30 
Average proteome isoelectric point is 7.06 
Get precalculated fractions of proteins 
 
  
    Acidic  
     
   
 
  
    pI < 6.8  
     
   
 
  
    6.8-7.4  
     
   
 
  
    pI > 7.4  
     
   
 
  
    Basic  
     
   
    
 
  
    All  
     
   
 
 
Note: above files contain also dissociation constants (pKa) 
 
Virtual 2D-PAGE plot for 177 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 
 Summary statistics related to proteome-wise predictions 
 
Protein with the lowest isoelectric point: 
>tr|W8W1Q7|W8W1Q7_9VIRU Isoform of W8W1Q3  Uncharacterized protein OS=Invertebrate iridescent virus 30 OX=345585 GN=053R PE=4 SV=1 
MM1 pKa = 7.73  LPTTSINDD9 pKa = 3.67  SGDD12 pKa = 3.4  CTLSLKK18 pKa = 10.01  TNEE21 pKa = 4.24  ISLTYY26 pKa = 10.47  YY27 pKa = 10.78  FEE29 pKa = 4.91  NNNDD33 pKa = 3.75  LFDD36 pKa = 5.54  LGIALLKK43 pKa = 10.74  NSLISKK49 pKa = 10.07  IKK51 pKa = 10.47  VEE53 pKa = 4.21  NNLEE57 pKa = 4.0  IGEE60 pKa = 4.33  TTDD63 pKa = 3.54  VIEE66 pKa = 4.79  KK67 pKa = 9.6  FISSITQNLKK77 pKa = 10.84  DD78 pKa = 5.21  DD79 pKa = 3.95  LTFQNKK85 pKa = 8.92  IEE87 pKa = 3.97  QHH89 pKa = 5.95  EE90 pKa = 4.48  IYY92 pKa = 10.81  YY93 pKa = 10.28  SDD95 pKa = 3.95  SEE97 pKa = 4.59  SDD99 pKa = 3.65  IKK101 pKa = 11.14  DD102 pKa = 3.83  DD103 pKa = 4.93  SPLITDD109 pKa = 4.61  DD110 pKa = 3.95  EE111 pKa = 5.48  SNDD114 pKa = 3.48  EE115 pKa = 4.27  ILNITKK121 pKa = 10.5  SMEE124 pKa = 3.6  KK125 pKa = 10.24  SAIISDD131 pKa = 4.64  DD132 pKa = 3.35  EE133 pKa = 4.35  DD134 pKa = 3.44   
 Molecular weight: 15.15 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  4.218 
IPC2_protein 3.872 
IPC_protein 3.846 
Toseland    3.643 
ProMoST     3.961 
Dawson      3.834 
Bjellqvist  4.024 
Wikipedia   3.757 
Rodwell     3.681 
Grimsley    3.554 
Solomon     3.821 
Lehninger   3.783 
Nozaki      3.948 
DTASelect   4.164 
Thurlkill   3.694 
EMBOSS      3.77 
Sillero     3.973 
Patrickios  3.579 
IPC_peptide 3.821 
IPC2_peptide  3.948 
IPC2.peptide.svr19  3.899 
 Protein with the highest isoelectric point: 
>tr|W8W277|W8W277_9VIRU Thymidine kinase OS=Invertebrate iridescent virus 30 OX=345585 GN=156R PE=4 SV=1 
MM1 pKa = 7.56  TSNCAKK7 pKa = 9.36  WHH9 pKa = 5.27  SQPLVNPVTNRR20 pKa = 11.84  KK21 pKa = 9.09  IKK23 pKa = 10.84  VGGPKK28 pKa = 10.2  YY29 pKa = 10.43  KK30 pKa = 10.34  EE31 pKa = 4.49  LEE33 pKa = 4.46  QKK35 pKa = 10.56  CGPPPSRR42 pKa = 11.84  QSRR45 pKa = 11.84  RR46 pKa = 11.84  SPSPSRR52 pKa = 11.84  QSRR55 pKa = 11.84  RR56 pKa = 11.84  SPSPSRR62 pKa = 11.84  QSRR65 pKa = 11.84  RR66 pKa = 11.84  SPPPSRR72 pKa = 11.84  QSRR75 pKa = 11.84  RR76 pKa = 11.84  SPSPSRR82 pKa = 11.84  RR83 pKa = 11.84  SRR85 pKa = 11.84  RR86 pKa = 11.84  SPSPSRR92 pKa = 11.84  QSRR95 pKa = 11.84  RR96 pKa = 11.84  SPSPSRR102 pKa = 11.84  RR103 pKa = 11.84  PTEE106 pKa = 3.89  IYY108 pKa = 10.27  CGNNARR114 pKa = 11.84  DD115 pKa = 3.45  EE116 pKa = 4.17  GLINGTKK123 pKa = 10.0  ILGTRR128 pKa = 11.84  YY129 pKa = 8.96  QCLKK133 pKa = 10.44  KK134 pKa = 10.8  GIGRR138 pKa = 11.84  GLHH141 pKa = 5.82  EE142 pKa = 5.99  PILSYY147 pKa = 11.18  TNDD150 pKa = 3.29  YY151 pKa = 11.29  SPIEE155 pKa = 3.96  EE156 pKa = 4.02  VKK158 pKa = 10.51  IFCGNGNVLPQNKK171 pKa = 9.39  DD172 pKa = 2.83  RR173 pKa = 11.84  FGTRR177 pKa = 11.84  DD178 pKa = 2.85  EE179 pKa = 4.3  CLRR182 pKa = 11.84  KK183 pKa = 10.23  GFAVGQNQKK192 pKa = 7.56  YY193 pKa = 7.51  TRR195 pKa = 11.84  DD196 pKa = 3.23  GDD198 pKa = 3.73  IQRR201 pKa = 11.84  TPVVSEE207 pKa = 3.75  DD208 pKa = 3.23  KK209 pKa = 10.87  GWYY212 pKa = 9.1  KK213 pKa = 10.95  VYY215 pKa = 10.69  LPSALGAPILRR226 pKa = 11.84  RR227 pKa = 3.57   
 Molecular weight: 25.65 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.286 
IPC2_protein 9.955 
IPC_protein 11.038 
Toseland    11.125 
ProMoST     11.374 
Dawson      11.184 
Bjellqvist  11.023 
Wikipedia   11.506 
Rodwell     11.228 
Grimsley    11.228 
Solomon     11.477 
Lehninger   11.403 
Nozaki      11.125 
DTASelect   11.023 
Thurlkill   11.125 
EMBOSS      11.579 
Sillero     11.14 
Patrickios  10.935 
IPC_peptide 11.477 
IPC2_peptide  10.365 
IPC2.peptide.svr19  8.771 
  
 
Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try ESI  
     
   
 
  
    ChTry ESI  
     
   
 
  
    ArgC ESI  
     
   
 
  
    LysN ESI  
     
   
 
  
    TryLysC ESI  
     
   
 
  
    Try MALDI  
     
   
 
  
    ChTry MALDI  
     
   
 
  
    ArgC MALDI  
     
   
 
  
    LysN MALDI  
     
   
 
  
    TryLysC MALDI  
     
   
 
  
    Try LTQ  
     
   
 
  
    ChTry LTQ  
     
   
 
  
    ArgC LTQ  
     
   
 
  
    LysN LTQ  
     
   
 
  
    TryLysC LTQ  
     
   
 
  
    Try MSlow  
     
   
 
  
    ChTry MSlow  
     
   
 
  
    ArgC MSlow  
     
   
 
  
    LysN MSlow  
     
   
 
  
    TryLysC MSlow  
     
   
 
  
    Try MShigh  
     
   
 
  
    ChTry MShigh  
     
   
 
  
    ArgC MShigh  
     
   
 
  
    LysN MShigh  
     
   
 
  
    TryLysC MShigh  
     
   
   
  
                       
General Statistics 
    
      
        Number of major isoforms
 
        Number of additional isoforms
 
        Number of all proteins
         
        Number of amino acids
 
        Min. Seq. Length
 
        Max. Seq. Length
 
        Avg. Seq. Length
 
        Avg. Mol. Weight
 
       
     
    
      
        176 
 
        
        1 
 
        
        177 
         
        58589
 
        49
 
        1591
 
        331.0
 
        37.74
 
                
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
 
        Cys
 
        Asp
 
        Glu
 
        Phe
 
        Gly
 
        His
 
        Ile
 
        Lys
 
        Leu
 
       
     
    
                 
        3.752 ± 0.267
1.739 ± 0.115
 
        5.382 ± 0.126
6.093 ± 0.257
 
        4.69 ± 0.162
4.842 ± 0.236
 
        1.625 ± 0.09
8.179 ± 0.197
       
        9.34 ± 0.286
9.485 ± 0.185
         
                
     
  
  
  
      
          
        Met
         
        Asn
 
        Gln
 
        Pro
         
        Arg
 
        Ser
 
        Thr
 
        Val
 
        Trp
 
        Tyr
  
       
     
    
                 
        2.009 ± 0.079
7.268 ± 0.148
 
        4.199 ± 0.234
3.874 ± 0.105
 
        3.053 ± 0.156
7.351 ± 0.353
 
        6.542 ± 0.281
5.634 ± 0.165
       
        0.814 ± 0.043
4.129 ± 0.157
         
                
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level 
Most of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here