Invertebrate iridescent virus 30
Average proteome isoelectric point is 7.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 177 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W8W1Q7|W8W1Q7_9VIRU Isoform of W8W1Q3 Uncharacterized protein OS=Invertebrate iridescent virus 30 OX=345585 GN=053R PE=4 SV=1
MM1 pKa = 7.73 LPTTSINDD9 pKa = 3.67 SGDD12 pKa = 3.4 CTLSLKK18 pKa = 10.01 TNEE21 pKa = 4.24 ISLTYY26 pKa = 10.47 YY27 pKa = 10.78 FEE29 pKa = 4.91 NNNDD33 pKa = 3.75 LFDD36 pKa = 5.54 LGIALLKK43 pKa = 10.74 NSLISKK49 pKa = 10.07 IKK51 pKa = 10.47 VEE53 pKa = 4.21 NNLEE57 pKa = 4.0 IGEE60 pKa = 4.33 TTDD63 pKa = 3.54 VIEE66 pKa = 4.79 KK67 pKa = 9.6 FISSITQNLKK77 pKa = 10.84 DD78 pKa = 5.21 DD79 pKa = 3.95 LTFQNKK85 pKa = 8.92 IEE87 pKa = 3.97 QHH89 pKa = 5.95 EE90 pKa = 4.48 IYY92 pKa = 10.81 YY93 pKa = 10.28 SDD95 pKa = 3.95 SEE97 pKa = 4.59 SDD99 pKa = 3.65 IKK101 pKa = 11.14 DD102 pKa = 3.83 DD103 pKa = 4.93 SPLITDD109 pKa = 4.61 DD110 pKa = 3.95 EE111 pKa = 5.48 SNDD114 pKa = 3.48 EE115 pKa = 4.27 ILNITKK121 pKa = 10.5 SMEE124 pKa = 3.6 KK125 pKa = 10.24 SAIISDD131 pKa = 4.64 DD132 pKa = 3.35 EE133 pKa = 4.35 DD134 pKa = 3.44
Molecular weight: 15.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.218
IPC2_protein 3.872
IPC_protein 3.846
Toseland 3.643
ProMoST 3.961
Dawson 3.834
Bjellqvist 4.024
Wikipedia 3.757
Rodwell 3.681
Grimsley 3.554
Solomon 3.821
Lehninger 3.783
Nozaki 3.948
DTASelect 4.164
Thurlkill 3.694
EMBOSS 3.77
Sillero 3.973
Patrickios 3.579
IPC_peptide 3.821
IPC2_peptide 3.948
IPC2.peptide.svr19 3.899
Protein with the highest isoelectric point:
>tr|W8W277|W8W277_9VIRU Thymidine kinase OS=Invertebrate iridescent virus 30 OX=345585 GN=156R PE=4 SV=1
MM1 pKa = 7.56 TSNCAKK7 pKa = 9.36 WHH9 pKa = 5.27 SQPLVNPVTNRR20 pKa = 11.84 KK21 pKa = 9.09 IKK23 pKa = 10.84 VGGPKK28 pKa = 10.2 YY29 pKa = 10.43 KK30 pKa = 10.34 EE31 pKa = 4.49 LEE33 pKa = 4.46 QKK35 pKa = 10.56 CGPPPSRR42 pKa = 11.84 QSRR45 pKa = 11.84 RR46 pKa = 11.84 SPSPSRR52 pKa = 11.84 QSRR55 pKa = 11.84 RR56 pKa = 11.84 SPSPSRR62 pKa = 11.84 QSRR65 pKa = 11.84 RR66 pKa = 11.84 SPPPSRR72 pKa = 11.84 QSRR75 pKa = 11.84 RR76 pKa = 11.84 SPSPSRR82 pKa = 11.84 RR83 pKa = 11.84 SRR85 pKa = 11.84 RR86 pKa = 11.84 SPSPSRR92 pKa = 11.84 QSRR95 pKa = 11.84 RR96 pKa = 11.84 SPSPSRR102 pKa = 11.84 RR103 pKa = 11.84 PTEE106 pKa = 3.89 IYY108 pKa = 10.27 CGNNARR114 pKa = 11.84 DD115 pKa = 3.45 EE116 pKa = 4.17 GLINGTKK123 pKa = 10.0 ILGTRR128 pKa = 11.84 YY129 pKa = 8.96 QCLKK133 pKa = 10.44 KK134 pKa = 10.8 GIGRR138 pKa = 11.84 GLHH141 pKa = 5.82 EE142 pKa = 5.99 PILSYY147 pKa = 11.18 TNDD150 pKa = 3.29 YY151 pKa = 11.29 SPIEE155 pKa = 3.96 EE156 pKa = 4.02 VKK158 pKa = 10.51 IFCGNGNVLPQNKK171 pKa = 9.39 DD172 pKa = 2.83 RR173 pKa = 11.84 FGTRR177 pKa = 11.84 DD178 pKa = 2.85 EE179 pKa = 4.3 CLRR182 pKa = 11.84 KK183 pKa = 10.23 GFAVGQNQKK192 pKa = 7.56 YY193 pKa = 7.51 TRR195 pKa = 11.84 DD196 pKa = 3.23 GDD198 pKa = 3.73 IQRR201 pKa = 11.84 TPVVSEE207 pKa = 3.75 DD208 pKa = 3.23 KK209 pKa = 10.87 GWYY212 pKa = 9.1 KK213 pKa = 10.95 VYY215 pKa = 10.69 LPSALGAPILRR226 pKa = 11.84 RR227 pKa = 3.57
Molecular weight: 25.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.286
IPC2_protein 9.955
IPC_protein 11.038
Toseland 11.125
ProMoST 11.374
Dawson 11.184
Bjellqvist 11.023
Wikipedia 11.506
Rodwell 11.228
Grimsley 11.228
Solomon 11.477
Lehninger 11.403
Nozaki 11.125
DTASelect 11.023
Thurlkill 11.125
EMBOSS 11.579
Sillero 11.14
Patrickios 10.935
IPC_peptide 11.477
IPC2_peptide 10.365
IPC2.peptide.svr19 8.771
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
176
1
177
58589
49
1591
331.0
37.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.752 ± 0.267
1.739 ± 0.115
5.382 ± 0.126
6.093 ± 0.257
4.69 ± 0.162
4.842 ± 0.236
1.625 ± 0.09
8.179 ± 0.197
9.34 ± 0.286
9.485 ± 0.185
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.009 ± 0.079
7.268 ± 0.148
4.199 ± 0.234
3.874 ± 0.105
3.053 ± 0.156
7.351 ± 0.353
6.542 ± 0.281
5.634 ± 0.165
0.814 ± 0.043
4.129 ± 0.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here