Pseudidiomarina planktonica
Average proteome isoelectric point is 5.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2385 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y6E7Z5|A0A1Y6E7Z5_9GAMM Uncharacterized protein OS=Pseudidiomarina planktonica OX=1323738 GN=SAMN06297229_0167 PE=4 SV=1
MM1 pKa = 7.28 YY2 pKa = 10.81 SNLFNLSRR10 pKa = 11.84 IKK12 pKa = 10.71 NLLMLFLVSTVLAACGDD29 pKa = 3.8 RR30 pKa = 11.84 DD31 pKa = 4.65 PILGYY36 pKa = 10.5 DD37 pKa = 3.82 GNVALSPTVIAVTPADD53 pKa = 3.67 NATDD57 pKa = 3.62 VAINGASITARR68 pKa = 11.84 FSVPVEE74 pKa = 3.83 PLSNDD79 pKa = 3.62 DD80 pKa = 3.67 FTLACSDD87 pKa = 3.78 TCTSPSGTVSMNDD100 pKa = 3.08 AGTVATFTPVAPAVLDD116 pKa = 3.88 GSTLYY121 pKa = 10.19 TATVHH126 pKa = 5.96 YY127 pKa = 9.92 AQSKK131 pKa = 10.4 DD132 pKa = 3.31 NGLVLEE138 pKa = 4.9 NPYY141 pKa = 9.72 IWSFTTSVVPEE152 pKa = 4.43 TIRR155 pKa = 11.84 PRR157 pKa = 11.84 VTLTDD162 pKa = 3.7 PVTTSSGTTVDD173 pKa = 3.61 VPINTSVLAAFNEE186 pKa = 3.97 NMAPAMFTEE195 pKa = 4.31 ITFTLTCEE203 pKa = 4.54 APCMAPQGAVSYY215 pKa = 10.59 DD216 pKa = 3.51 VSAKK220 pKa = 10.16 AAQFKK225 pKa = 9.28 PAQDD229 pKa = 4.45 LEE231 pKa = 4.36 WEE233 pKa = 4.38 TTYY236 pKa = 10.6 TATISQAVTDD246 pKa = 4.11 LAGNEE251 pKa = 4.03 LAGNQGPLTEE261 pKa = 4.63 ASDD264 pKa = 4.23 YY265 pKa = 10.79 VWQFTTGSTPDD276 pKa = 3.05 ATRR279 pKa = 11.84 PRR281 pKa = 11.84 VTLTDD286 pKa = 3.84 PASTSPANVSVNTSVLAVFNEE307 pKa = 3.66 NMAPATFTDD316 pKa = 3.33 ATFTLTCDD324 pKa = 3.84 APCTGPQGAVSYY336 pKa = 10.64 DD337 pKa = 3.51 VSAKK341 pKa = 10.16 AAQFKK346 pKa = 9.28 PAQDD350 pKa = 4.45 LEE352 pKa = 4.36 WEE354 pKa = 4.38 TTYY357 pKa = 10.6 TATISQAVTDD367 pKa = 4.11 LAGNEE372 pKa = 3.96 LAGNQGSVTDD382 pKa = 4.1 PSDD385 pKa = 3.72 YY386 pKa = 11.17 AWEE389 pKa = 4.32 FTTGVAPDD397 pKa = 3.56 TTRR400 pKa = 11.84 PRR402 pKa = 11.84 VTLTNPATTSPEE414 pKa = 4.11 LEE416 pKa = 3.83 TDD418 pKa = 3.67 VPVNTSVLAVFNEE431 pKa = 3.61 NMVPSTFTDD440 pKa = 3.34 TTFTLTCDD448 pKa = 3.91 SPCATSLGTVSYY460 pKa = 11.15 NVDD463 pKa = 2.93 AKK465 pKa = 10.3 SARR468 pKa = 11.84 FVPAQDD474 pKa = 4.33 LEE476 pKa = 5.02 AEE478 pKa = 4.38 TTYY481 pKa = 10.44 TATISRR487 pKa = 11.84 SVTDD491 pKa = 3.55 LAGNEE496 pKa = 4.03 LAGNQGPLTDD506 pKa = 4.2 PSDD509 pKa = 4.09 YY510 pKa = 10.88 VWQFTTGLAPDD521 pKa = 3.77 TTRR524 pKa = 11.84 PRR526 pKa = 11.84 VTLTEE531 pKa = 4.33 PEE533 pKa = 4.45 TTSSGPTADD542 pKa = 3.59 VPVNTSVLAVFNEE555 pKa = 3.63 NMAPATLTDD564 pKa = 3.35 TTFTLTCDD572 pKa = 3.82 SPCAVPLGTVSYY584 pKa = 11.09 NVDD587 pKa = 2.93 AKK589 pKa = 10.28 SARR592 pKa = 11.84 FRR594 pKa = 11.84 PAQNLEE600 pKa = 3.79 AEE602 pKa = 4.5 TTYY605 pKa = 10.55 TATISQAVTDD615 pKa = 4.11 LAGNEE620 pKa = 3.96 LAGNQGSVTQPSDD633 pKa = 3.75 YY634 pKa = 10.76 VWQFTTGLTPDD645 pKa = 4.25 TIRR648 pKa = 11.84 PRR650 pKa = 11.84 VTLTEE655 pKa = 4.37 PATSSPGPTANAPVNTSVLAVFNEE679 pKa = 3.63 NMVPSMFTDD688 pKa = 3.39 TTFTLICEE696 pKa = 4.78 APCVAPVGTVSYY708 pKa = 11.22 NVDD711 pKa = 3.09 TKK713 pKa = 10.45 SARR716 pKa = 11.84 FSPAQDD722 pKa = 3.97 LEE724 pKa = 4.35 PEE726 pKa = 4.23 TTYY729 pKa = 10.47 TATISRR735 pKa = 11.84 SVTDD739 pKa = 3.55 LAGNEE744 pKa = 4.03 LAGNQGPVTDD754 pKa = 4.39 PSDD757 pKa = 3.89 YY758 pKa = 10.86 VWQFTTGLAPDD769 pKa = 3.77 TTRR772 pKa = 11.84 PRR774 pKa = 11.84 VTLTEE779 pKa = 4.23 PATTSPGPTADD790 pKa = 3.54 VPVNTQIKK798 pKa = 10.1 AIFSEE803 pKa = 5.15 AISPTTITGTSFTLTCEE820 pKa = 4.56 EE821 pKa = 5.09 PCTAPIGNVSYY832 pKa = 10.83 DD833 pKa = 3.37 VSSRR837 pKa = 11.84 SAVFTPEE844 pKa = 3.5 QNLAEE849 pKa = 4.6 GEE851 pKa = 4.62 TYY853 pKa = 9.63 TATLVSTITDD863 pKa = 3.88 LAGNRR868 pKa = 11.84 LAGNQGPVTSASDD881 pKa = 4.19 YY882 pKa = 9.59 IWSFTTTAPIAPTNISVLSTDD903 pKa = 3.97 PLDD906 pKa = 4.23 AGTMAVCPNASINATFDD923 pKa = 3.19 IPSGSRR929 pKa = 11.84 LDD931 pKa = 3.74 PATVNNMTFLIVEE944 pKa = 4.48 DD945 pKa = 4.56 ANPLISVLAEE955 pKa = 4.21 SIQVDD960 pKa = 3.83 VDD962 pKa = 3.31 TGTVVTFIPQEE973 pKa = 3.87 QLPEE977 pKa = 3.99 NVTYY981 pKa = 10.54 RR982 pKa = 11.84 VTLVGGSDD990 pKa = 3.74 GVKK993 pKa = 10.89 DD994 pKa = 4.15 LMLPGNEE1001 pKa = 4.17 MLDD1004 pKa = 3.67 DD1005 pKa = 4.1 YY1006 pKa = 11.5 VWTFTTVAPVEE1017 pKa = 4.48 SCLVPVNLQSAAPFGSFGGTAGITNQGVLTVINGDD1052 pKa = 2.94 IGTTGTSTIVTGFVSEE1068 pKa = 4.75 PGCEE1072 pKa = 3.97 YY1073 pKa = 10.72 TITTLNEE1080 pKa = 3.68 GQVNGKK1086 pKa = 9.22 IFTAPPPPTVSCPQDD1101 pKa = 3.13 GTAEE1105 pKa = 4.15 TEE1107 pKa = 4.58 AIATQARR1114 pKa = 11.84 LDD1116 pKa = 3.74 AEE1118 pKa = 4.18 SAYY1121 pKa = 11.25 NEE1123 pKa = 3.99 LTPANMPGGQNPGNEE1138 pKa = 4.11 NLGGLTLAPGIYY1150 pKa = 8.85 TAQSGAFRR1158 pKa = 11.84 IQGGDD1163 pKa = 3.37 LTLDD1167 pKa = 3.51 GQGNQNAVWVFQMATTLTVGGPGADD1192 pKa = 3.94 FSQSVTLINGAQAKK1206 pKa = 10.2 NIFWQVGSAATINAGGGGVMKK1227 pKa = 9.46 GTIIAQEE1234 pKa = 4.2 GVTISTAGNVDD1245 pKa = 2.7 IVTIDD1250 pKa = 3.3 GRR1252 pKa = 11.84 ALSLGASVTMVNTVINVPADD1272 pKa = 3.18
Molecular weight: 132.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.693
IPC2_protein 3.732
IPC_protein 3.77
Toseland 3.554
ProMoST 3.91
Dawson 3.757
Bjellqvist 3.923
Wikipedia 3.681
Rodwell 3.592
Grimsley 3.452
Solomon 3.757
Lehninger 3.706
Nozaki 3.859
DTASelect 4.101
Thurlkill 3.592
EMBOSS 3.681
Sillero 3.884
Patrickios 1.036
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.809
Protein with the highest isoelectric point:
>tr|A0A1Y6E641|A0A1Y6E641_9GAMM dGTPase OS=Pseudidiomarina planktonica OX=1323738 GN=SAMN06297229_0057 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.42 RR12 pKa = 11.84 KK13 pKa = 9.04 RR14 pKa = 11.84 AHH16 pKa = 6.12 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.94 GRR39 pKa = 11.84 KK40 pKa = 8.67 VLSAA44 pKa = 4.05
Molecular weight: 5.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2385
0
2385
795332
32
1611
333.5
36.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.892 ± 0.053
0.854 ± 0.015
5.67 ± 0.038
6.402 ± 0.051
3.827 ± 0.03
6.945 ± 0.045
2.217 ± 0.027
5.483 ± 0.039
4.15 ± 0.044
10.632 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.437 ± 0.024
3.766 ± 0.033
4.179 ± 0.031
5.264 ± 0.05
5.491 ± 0.034
6.057 ± 0.042
5.34 ± 0.034
7.209 ± 0.042
1.316 ± 0.023
2.867 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here