Blackberry yellow vein-associated virus
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q5ICV3|Q5ICV3_9CLOS p5 OS=Blackberry yellow vein-associated virus OX=404196 PE=4 SV=1
MM1 pKa = 7.5 LVQFFFTNDD10 pKa = 2.85 IGVTIYY16 pKa = 11.0 YY17 pKa = 10.69 LSDD20 pKa = 3.1 NKK22 pKa = 10.56 NFEE25 pKa = 4.26 GEE27 pKa = 4.16 IFTVTSSDD35 pKa = 3.32 QSLLLDD41 pKa = 4.02 LLVVFPYY48 pKa = 10.55 LRR50 pKa = 11.84 EE51 pKa = 3.71 RR52 pKa = 11.84 WW53 pKa = 3.53
Molecular weight: 6.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.208
IPC2_protein 4.215
IPC_protein 3.986
Toseland 3.808
ProMoST 4.164
Dawson 3.973
Bjellqvist 4.139
Wikipedia 3.935
Rodwell 3.821
Grimsley 3.732
Solomon 3.948
Lehninger 3.897
Nozaki 4.126
DTASelect 4.291
Thurlkill 3.884
EMBOSS 3.935
Sillero 4.113
Patrickios 3.35
IPC_peptide 3.948
IPC2_peptide 4.075
IPC2.peptide.svr19 4.023
Protein with the highest isoelectric point:
>tr|Q5ICV6|Q5ICV6_9CLOS p27 OS=Blackberry yellow vein-associated virus OX=404196 PE=4 SV=1
MM1 pKa = 7.26 FWFFYY6 pKa = 11.06 LLLLLVVFIFFPCQSKK22 pKa = 9.81 SGYY25 pKa = 7.18 VVRR28 pKa = 11.84 SGRR31 pKa = 11.84 FSTDD35 pKa = 2.26
Molecular weight: 4.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.748
IPC2_protein 8.521
IPC_protein 9.063
Toseland 8.931
ProMoST 8.77
Dawson 9.121
Bjellqvist 9.18
Wikipedia 9.238
Rodwell 9.121
Grimsley 8.799
Solomon 9.575
Lehninger 9.56
Nozaki 9.238
DTASelect 9.048
Thurlkill 9.048
EMBOSS 9.297
Sillero 9.297
Patrickios 4.202
IPC_peptide 9.545
IPC2_peptide 8.536
IPC2.peptide.svr19 7.889
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11
0
11
6806
35
2435
618.7
70.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.011 ± 0.253
2.366 ± 0.326
6.156 ± 0.387
5.613 ± 0.339
6.259 ± 0.506
4.584 ± 0.282
1.616 ± 0.225
6.582 ± 0.253
8.022 ± 0.522
9.051 ± 0.54
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.601 ± 0.165
5.951 ± 0.39
2.953 ± 0.128
2.542 ± 0.244
4.775 ± 0.132
9.286 ± 0.516
5.231 ± 0.322
7.611 ± 0.227
0.646 ± 0.052
4.143 ± 0.16
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here