Lactobacillus ghanensis DSM 18630
Average proteome isoelectric point is 7.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2384 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R1VY18|A0A0R1VY18_9LACO Acylphosphatase OS=Lactobacillus ghanensis DSM 18630 OX=1423750 GN=FC89_GL000158 PE=3 SV=1
MM1 pKa = 7.61 TEE3 pKa = 4.42 RR4 pKa = 11.84 NDD6 pKa = 3.64 NSSTALAQFANSAFFDD22 pKa = 3.44 QSFPKK27 pKa = 10.34 QSQDD31 pKa = 2.85 YY32 pKa = 11.11 DD33 pKa = 3.98 EE34 pKa = 4.78 ISNYY38 pKa = 10.59 LEE40 pKa = 4.79 LNAGYY45 pKa = 9.42 LPSMTIFDD53 pKa = 4.24 QAWQQYY59 pKa = 7.48 LEE61 pKa = 4.22 KK62 pKa = 10.33 MEE64 pKa = 4.18
Molecular weight: 7.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.782
IPC2_protein 3.923
IPC_protein 3.77
Toseland 3.592
ProMoST 3.884
Dawson 3.757
Bjellqvist 4.037
Wikipedia 3.706
Rodwell 3.617
Grimsley 3.516
Solomon 3.719
Lehninger 3.681
Nozaki 3.91
DTASelect 4.05
Thurlkill 3.668
EMBOSS 3.719
Sillero 3.884
Patrickios 1.875
IPC_peptide 3.719
IPC2_peptide 3.859
IPC2.peptide.svr19 3.859
Protein with the highest isoelectric point:
>tr|A0A0R1VI38|A0A0R1VI38_9LACO Lipoprotein OS=Lactobacillus ghanensis DSM 18630 OX=1423750 GN=FC89_GL001775 PE=4 SV=1
MM1 pKa = 7.57 KK2 pKa = 9.74 TRR4 pKa = 11.84 QVQVLRR10 pKa = 11.84 IQQVRR15 pKa = 11.84 LLLIKK20 pKa = 9.67 EE21 pKa = 4.44 LKK23 pKa = 10.28 AVQKK27 pKa = 10.06 QAAVRR32 pKa = 11.84 IRR34 pKa = 11.84 QVQVLQIQQVHH45 pKa = 6.45 LLLIKK50 pKa = 9.75 EE51 pKa = 4.52 LKK53 pKa = 9.26 TVQQQATARR62 pKa = 11.84 IRR64 pKa = 11.84 QVQVLRR70 pKa = 11.84 IQQVHH75 pKa = 6.57 LLLIKK80 pKa = 9.78 EE81 pKa = 4.45 LKK83 pKa = 9.19 TVQKK87 pKa = 10.23 QAAARR92 pKa = 11.84 IRR94 pKa = 11.84 QVQVLQIQKK103 pKa = 8.67 TALRR107 pKa = 11.84 LIKK110 pKa = 9.9 KK111 pKa = 9.66 LKK113 pKa = 10.11 AIRR116 pKa = 11.84 RR117 pKa = 11.84 LQSSVQVMTQVLPQLLVRR135 pKa = 11.84 AVQQRR140 pKa = 11.84 RR141 pKa = 11.84 PKK143 pKa = 10.27 RR144 pKa = 11.84 HH145 pKa = 6.52 SLLQNQWQKK154 pKa = 10.8 RR155 pKa = 11.84 PRR157 pKa = 11.84 VLHH160 pKa = 5.88 HH161 pKa = 5.8 QHH163 pKa = 6.2 HH164 pKa = 6.04 QLSKK168 pKa = 9.14 QQLIKK173 pKa = 10.83 LVLLKK178 pKa = 10.41 LVPKK182 pKa = 10.56 LVFLPAQAVRR192 pKa = 11.84 QRR194 pKa = 11.84 LFFIPRR200 pKa = 11.84 KK201 pKa = 9.7 RR202 pKa = 11.84 PLLSWQRR209 pKa = 3.4
Molecular weight: 24.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.446
IPC2_protein 10.979
IPC_protein 12.544
Toseland 12.72
ProMoST 13.203
Dawson 12.72
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.501
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.208
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.06
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2384
0
2384
747846
45
2068
313.7
35.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.265 ± 0.065
0.645 ± 0.016
4.883 ± 0.044
5.105 ± 0.047
4.465 ± 0.04
6.45 ± 0.049
2.001 ± 0.025
7.401 ± 0.053
7.035 ± 0.054
10.617 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.326 ± 0.024
4.879 ± 0.044
3.532 ± 0.026
5.51 ± 0.062
3.824 ± 0.036
5.989 ± 0.05
5.859 ± 0.042
6.619 ± 0.045
1.051 ± 0.019
3.52 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here