Gordonia phage GMA3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Gamtrevirus; Gordonia virus GMA3

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 104 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K0NKN6|A0A0K0NKN6_9CAUD Uncharacterized protein OS=Gordonia phage GMA3 OX=1647284 GN=GMA3_99 PE=4 SV=1
MM1 pKa = 7.25LWIVPIDD8 pKa = 4.1ADD10 pKa = 3.19IDD12 pKa = 3.87DD13 pKa = 4.6YY14 pKa = 11.81ARR16 pKa = 11.84EE17 pKa = 4.08WVDD20 pKa = 3.32YY21 pKa = 10.26FAGDD25 pKa = 3.36VYY27 pKa = 11.26GAIVDD32 pKa = 3.83SDD34 pKa = 4.55DD35 pKa = 3.74YY36 pKa = 11.56AISDD40 pKa = 3.5NSVWGVYY47 pKa = 10.6GDD49 pKa = 4.31DD50 pKa = 3.77YY51 pKa = 12.16AMQTLSEE58 pKa = 4.28YY59 pKa = 10.49VRR61 pKa = 11.84YY62 pKa = 9.49ALEE65 pKa = 5.05EE66 pKa = 3.72ISQTIKK72 pKa = 10.82DD73 pKa = 4.06NKK75 pKa = 8.85RR76 pKa = 11.84KK77 pKa = 10.11AMCAA81 pKa = 3.29

Molecular weight: 9.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K0NKV5|A0A0K0NKV5_9CAUD Putative holin OS=Gordonia phage GMA3 OX=1647284 GN=GMA3_27 PE=4 SV=1
MM1 pKa = 7.21KK2 pKa = 10.29RR3 pKa = 11.84LYY5 pKa = 9.98RR6 pKa = 11.84VKK8 pKa = 10.34IKK10 pKa = 10.59FNNGSTMRR18 pKa = 11.84FSAEE22 pKa = 3.78EE23 pKa = 3.56FTLRR27 pKa = 11.84SNGASATWRR36 pKa = 11.84NTIPNLVDD44 pKa = 4.63LDD46 pKa = 4.53LDD48 pKa = 4.31NITYY52 pKa = 7.55ATSRR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 11.84PIWYY62 pKa = 9.14IMKK65 pKa = 9.45MLGKK69 pKa = 10.13VV70 pKa = 3.07

Molecular weight: 8.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

104

0

104

24212

37

3186

232.8

25.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.169 ± 0.775

0.966 ± 0.175

6.542 ± 0.301

6.137 ± 0.354

3.725 ± 0.148

8.211 ± 0.505

1.569 ± 0.22

6.084 ± 0.179

5.729 ± 0.259

6.972 ± 0.238

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.796 ± 0.132

4.803 ± 0.158

4.659 ± 0.266

3.498 ± 0.167

5.303 ± 0.344

6.522 ± 0.18

6.014 ± 0.249

6.625 ± 0.188

1.582 ± 0.097

3.094 ± 0.21
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski